Effects of temperature and cellular interactions on the mechanics and morphology of human cancer cells investigated by atomic force microscopy
查看参考文献47篇
|
文摘
|
Cell mechanics plays an important role in cellular physiological activities. Recent studies have shown that cellular mechanical properties are novel biomarkers for indicating the cell states. In this article, temperature-controllable atomic force microscopy (AFM) was applied to quantitatively investigate the effects of temperature and cellular interactions on the mechanics and morphology of human cancer cells. First, AFM indenting experiments were performed on six types of human cells to investigate the changes of cellular Young's modulus at different temperatures and the results showed that the mechanical responses to the changes of temperature were variable for different types of cancer cells. Second, AFM imaging experiments were performed to observe the morphological changes in living cells at different temperatures and the results showed the significant changes of cell morphology caused by the alterations of temperature. Finally, by co-culturing human cancer cells with human immune cells, the mechanical and morphological changes in cancer cells were investigated. The results showed that the co-culture of cancer cells and immune cells could cause the distinct mechanical changes in cancer cells, but no significant morphological differences were observed. The experimental results improved our understanding of the effects of temperature and cellular interactions on the mechanics and morphology of cancer cells. |
|
来源
|
Science China. Life Sciences
,2015,58(9):889-901 【核心库】
|
|
DOI
|
10.1007/s11427-015-4914-4
|
|
关键词
|
atomic force microscopy
;
mechanics
;
cancer cell
;
temperature
;
cellular interactions
|
|
地址
|
1.
Shenyang Institute of Automation, Chinese Academy of Sciences, State Key Laboratory of Robotics, Shenyang, 110016
2.
Department of Lymphoma, Affiliated Hospital of Military Medical Academy of Sciences, Beijing, 100071
|
|
语种
|
英文 |
|
文献类型
|
研究性论文 |
|
ISSN
|
1674-7305 |
|
学科
|
肿瘤学 |
|
基金
|
国家自然科学基金
;
the Research Fund of the State Key Laboratory of Robotics
;
CAS FEA International Partnership Program for Creative Research Teams
|
|
文献收藏号
|
CSCD:5530132
|
参考文献 共
47
共3页
|
|
1.
Suresh S. Biomechanics and biophysics of cancer cells.
Acta Biomater,2007,3:413-438
|
被引
30
次
|
|
|
|
|
2.
Koumoutsakos P. The fluid mechanics of cancer and its therapy.
Ann Rev Fluid Mech,2013,45:325-355
|
被引
10
次
|
|
|
|
|
3.
Lee G Y. Biomechanics approaches to studying human diseases.
Trends Biotechnol,2007,25:111-118
|
被引
26
次
|
|
|
|
|
4.
Perrault C M. Altered rheology of lymphocytes in the diabetic mouse.
Diabetologia,2004,47:1722-1726
|
被引
1
次
|
|
|
|
|
5.
Trickey W R. The role of the cytoskeleton in the viscoelastic properties of human articular chondrocytes.
J Orthop Res,2004,22:131-139
|
被引
20
次
|
|
|
|
|
6.
Tomioka S. Airway and tissue mechanics in a murine model of asthma: alveolar capsule vs. forced oscillations.
J Appl Physiol,2002,93:263-270
|
被引
2
次
|
|
|
|
|
7.
Zheng Y. Recent advances in microfluidic techniques for single-cell biophysical characterization.
Lab Chip,2013,13:2464-2483
|
被引
6
次
|
|
|
|
|
8.
Fritsch A. Are biomechanical changes necessary for tumour progression.
Nat Phys,2010,6:730-732
|
被引
5
次
|
|
|
|
|
9.
Efremov Y M. Mechanical properties of firbroblasts depend on level of cancer transformation.
Biochim Biophys Acta,2014,1843:1013-1019
|
被引
5
次
|
|
|
|
|
10.
Plodinec M. The nanomechanical signature of breast cancer.
Nat Nanotechnol,2012,7:757-765
|
被引
38
次
|
|
|
|
|
11.
Wirtz D. The physics of cancer: the role of physical interactions and mechanical forces in metastasis.
Nat Rev Cancer,2011,11:512-522
|
被引
29
次
|
|
|
|
|
12.
Lekka M. Cancer cell recognition-mechanical phenotype.
Micron,2012,43:1259-1266
|
被引
16
次
|
|
|
|
|
13.
Rico F. Temperature modulation of integrin-mediated cell adhesion.
Biophys J,2010,99:1387-1396
|
被引
1
次
|
|
|
|
|
14.
Spedden E. Temperature response of the neuronal cytoskeleton mapped via atomic force and fluorescence microscopy.
Phys Biol,2013,10:056002
|
被引
1
次
|
|
|
|
|
15.
Junttila M R. Influence of tumour micro-environment heterogeneity on the therapeutic response.
Nature,2013,501:346-354
|
被引
94
次
|
|
|
|
|
16.
Li M. Atomic force microscopy imaging and mechanical properties measurement of red blood cells and aggressive cancer cells.
Sci China Life Sci,2012,55:968-973
|
被引
11
次
|
|
|
|
|
17.
Li M. Nanoscale imaging and mechanical analysis of Fc receptor-mediated macrophage phagocytosis against cancer cells.
Langmuir,2014,30:1609-1621
|
被引
4
次
|
|
|
|
|
18.
Li M. Progress in measuring biophysical properties of membrane proteins with AFM single-molecule force spectroscopy.
Chin Sci Bull,2014,59:2717-2725
|
被引
7
次
|
|
|
|
|
19.
Lingwood D. Lipid rafts as a membrane-organizing principle.
Science,2010,327:46-50
|
被引
55
次
|
|
|
|
|
20.
Gavara N. Determination of the elastic moduli of thin samples and adherent cells using conical atomic force microscope tips.
Nat Nanotechnol,2012,7:733-736
|
被引
14
次
|
|
|
|
|
了解气象卫星更多信息，请访问