榅椁总多酚的纯化工艺及PTP1B抑制作用研究
Purification and PTP1B Inhibition Activity of Total Polyphenols from Cydonia oblonga Mill
查看参考文献16篇
|
文摘
|
通过采用11种大孔吸附树脂对榅桲多酚粗提物的静态吸附和解吸试验,筛选出适合分离纯化榅桲总多酚的最优树脂,并对其动态吸附特性和影响因素进行研究。结果表明:最佳纯化工艺条件为:AB-8大孔吸附树脂做吸附填料,上样液质量浓度0.04 g/mL,以2 BV/h的吸附速率进行吸附,上样量为11 BV,用3 BV蒸馏水除杂后,以50%乙醇溶液为洗脱剂,洗脱液用量为4 BV,洗脱流速控制在2 BV/h。经AB-8树脂吸附富集后,样品总多酚质量浓度达到了30.11 mg/g,是粗提物(9.55 mg/g)的3倍。对纯化前后的榅桲总多酚进行PTP1B的抑制作用研究,结果显示,榅桲总多酚对PTP1B有较强的抑制作用,即榅桲总多酚纯化前的IC_950)为78.14 μg/mL,用AB-8树脂纯化后所得总多酚的IC_(50)为16.12 μg/mL。 |
|
其他语种文摘
|
In this study, 11 types of macroporous resins were screened for the purification of Cydonia oblonga Mill. polyphenols. The optimal resin was selected through static absorption and desorption experiments. Furthermore, its dynamic absorption features and influencing factors were analyzed. The results showed that the optimal conditions for purification were as follows : macroporous resin AB-8 had the best separation efficiency when the concentration of sample solution was 0.04 g/mL,the adsorption flow rate was 2 BV/h,the loading weight was 11 BV,removing impurities by 3 BV of distilled water and eluting the total polyphenols with 4 BV of 50% ethanol-water at flow rate of 2 BV/h. The concentration of total polyphenols of the product after the adsorption concentrating on AB-8 resin achieved 30.11 mg/g,which was 3 times of crude extract (9.55 mg/g). The PTP1B inhibition effect of polyphenol extracts before and after the purification process was also discussed. The results indicated that,the total polyphenols of C. oblonga had strong inhibition effect on PIP1B with IC_(50) value of 16.12 μg/mL after purification by AB-8 resin and the IC_(50) value was 78.14 μg/mL before purification. |
|
来源
|
天然产物研究与开发
,2015,27(8):1448-1452,1384 【核心库】
|
|
DOI
|
10.16333/j.1001-6880.2015.08.025
|
|
关键词
|
榅桲
;
AB-8大孔吸附树脂
;
总多酚
;
纯化
;
PTP1B抑制作用
|
|
地址
|
1.
新疆医科大学药学院
2.
中国科学院新疆理化技术研究所, 中国科学院干旱区植物资源化学重点实验室, 乌鲁木齐, 830011
|
|
语种
|
中文 |
|
文献类型
|
研究性论文 |
|
ISSN
|
1001-6880 |
|
学科
|
中国医学 |
|
基金
|
国家自然科学基金
;
新疆“天山英才工程”项目
;
新疆医科大学博士科研启动基金
|
|
文献收藏号
|
CSCD:5507788
|
参考文献 共
16
共1页
|
|
1.
新疆植物志编辑委员会.
新疆植物志. Vol II,1995:287
|
被引
1
次
|
|
|
|
|
2.
国家药典委员会.
中华人民共和国卫生部药品标准,维吾尔药分册,1999:105
|
被引
1
次
|
|
|
|
|
3.
中国医学百科全书编辑委员会.
中国医学百科全书维吾尔医学,2005:177
|
被引
2
次
|
|
|
|
|
4.
刘勇民.
维吾尔药志, Vol I,1999:538
|
被引
1
次
|
|
|
|
|
5.
Branca M S. Influence of jam processing upon the contents of phenolics, organic acids and free amino acids in quince fruit (Cydonia oblonga Miller).
Eur Food Res Technol,2004,218:385-389
|
被引
1
次
|
|
|
|
|
6.
Silva B M. Phenolic profile of quince fruit (Cydonia oblanga Miller) (Pulp and Peel).
J Agric Food Chem,2002,50:4615-4618
|
被引
2
次
|
|
|
|
|
7.
Tuba E. Dimeric procyanidins screening for B1 to B8 and semisynthetic preparation of B3, B4, B6, and B8 from a polymeric procyanidin fraction of white willow bark (Salix alba).
J Agric Food Chem,2010,58:7820-7830
|
被引
1
次
|
|
|
|
|
8.
Silva B M. Quince (Cydonia oblanga Miller) fruit (Pulp,Peel and seed) and jam:antioxidant activity.
J Agric Food Chem,2004,52:4405-4712
|
被引
3
次
|
|
|
|
|
9.
Hamauzu Y. Antioxidant and antiulcerative properties of phenolics from Chinese quince,quince, and apple fruits.
J Agric Food Chem,2006,54:765-772
|
被引
4
次
|
|
|
|
|
10.
Sami F. Antimicrobial activity of tunisian quince (Cydonia oblonga Miller) pulp and peel polyphenolic extracts.
J Agric Food Chem,2007,55:963-969
|
被引
5
次
|
|
|
|
|
11.
Antonio F. Isolation and structure elucidation of antioxidant polyphenols from quince (Cydonia vulgaris) peels.
J Agric Food Chem,2008,56:2660-2667
|
被引
4
次
|
|
|
|
|
12.
古丽斯玛依·艾拜都拉. Antibacterial effect of flavone extract from Xinjiang Cydonia oblonga Mill.
食品科学,2009,3:134-136
|
被引
1
次
|
|
|
|
|
13.
汪茂田.
天然有机化合物提取分离与结构鉴定,2004:100
|
被引
2
次
|
|
|
|
|
14.
热依木古丽·阿布都拉. A study on the preparation techniques of extracting polyphenols from the pericarpium granati (Punica granaum L.).
新疆医科大学学报,2013,36:723-728
|
被引
1
次
|
|
|
|
|
15.
胡辛欣. 鼠尾草属药用植物抑制蛋白酪氨酸磷酸酶1B活性初探.
药学学报,2009,44:440-442
|
被引
5
次
|
|
|
|
|
16.
林燕如. Study on the purification of flavonoid from the leaves of Cibotium baromeu (L.) J. Sm. by macroporous resin.
湖北匕农业科学,2011,50:583-586
|
被引
2
次
|
|
|
|
|
了解气象卫星更多信息，请访问