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Nanoscale monitoring of drug actions on cell membrane using atomic force microscopy

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文摘 Knowledge of the nanoscale changes that take place in individual cells in response to a drug is useful for understanding the drug action. However, due to the lack of adequate techniques, such knowledge was scarce until the advent of atomic force microscopy (AFM), which is a multifunctional tool for investigating cellular behavior with nanometer resolution under near-physiological conditions. In the past decade, researchers have applied AFM to monitor the morphological and mechanical dynamics of individual cells following drug stimulation, yielding considerable novel insight into how the drug molecules affect an individual cell at the nanoscale. In this article we summarize the representative applications of AFM in characterization of drug actions on cell membrane, including topographic imaging, elasticity measurements, molecular interaction quantification, native membrane protein imaging and manipulation, etc. The challenges that are hampering the further development of AFM for studies of cellular activities are aslo discussed.
来源 Acta Pharmacologica Sinica ,2015,36(7):769-782 【核心库】
DOI 10.1038/aps.2015.28
关键词 atomic force microscopy ; single-cell technique ; nanometer scale ; drug-target interaction ; cell mechanics ; cell elasticity
地址

Shenyang Institute of Automation, Chinese Academy of Sciences, State Key Laboratory of Robotics, Shenyang, 110016

语种 英文
文献类型 研究性论文
ISSN 1671-4083
基金 国家自然科学基金 ;  CAS FEA International Partnership Program for Creative Research Teams ;  the Research Fund of the State Key Laboratory of Robotics
文献收藏号 CSCD:5474147

参考文献 共 125 共7页

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