高通量测序研究种植体周围炎龈下微生物多样性
Study on Microbial Diversity of Peri-implantitis Subgingival by High-throughput Sequencing
查看参考文献15篇
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文摘
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目的利用高通量测序,研究口腔种植体周围炎龈下微生物的多样性,为种植体周围炎微生物学病因的研究提供思路。方法刮取种植体周围炎患者(D组)及正常种植修复患者(N组)龈下菌斑,利用Illumina Miseq测序平台,对16 SrRNA V4区进行双端测序;通过Mothur等软件进行群落结构的多样性分析。结果9个样本总测156 507条序列,共4 402个操作分类单位(OTU);在D组中的优势菌属为:新月形单胞菌属(Selenomonas)、假单胞菌属(Pseudomonas)及梭杆菌属(Fusobacterium)等,在N组中的优势菌属为:梭杆菌属、韦荣球菌属(Veillonella)及链球菌属(Streptococcus)等;PcoA检测显示两组微生物群落结构差异明显。LEfSe检测发现,在门、纲、目、科及属的水平上,两组均具有差异。结论种植体周围炎的发生发展不仅是牙周炎相关致病微生物的作用,而且与口腔微生物群落结构中菌属的变化有关;密螺旋体属(Treponema)、草螺菌属(Herbaspirillum)、Butyricimona及Phaeobacter等菌属在种植体周围炎及健康患者中存在差异,提示它们可能与种植体周围炎的发生发展密切相关。 |
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其他语种文摘
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Objective To study microbial diversity of peri-implantitis subgingival with high-throughput sequencing,and investigate microbiological etiology of peri-implantitis.Methods Subgingival plaques were sampled from the patients with peri-implantitis (D group) and non-peri-implantitis subjects (N group).The microbiological diversity of the subgingival plaques was detected by sequencing V4 region of 16S rRNA with Illumina Miseq platform.The diversity of the community structure was analyzed using Mothur software.Results A total of 156 507 gene sequences were detected in nine samples and 4 402 operational taxonomic units (OTUs) were found. Selenomonas,Pseudomonas,and Fusobacterium were dominant bacteria in D group,while Fusobacterium, Veillonella and Streptococcus were dominant bacteria in N group.Differences between peri-implantitis and non-periimplantitis bacterial communities were observed at all phylogenetic levels by LEfSe,which was also found in PcoA test.Conclusion The occurrence of peri-implantitis is not only related to periodontitis pathogenic microbe,but also related with the changes of oral microbial community structure.Treponema,Herbaspirillum,Butyricimonas and Phaeobacte may be closely related to the occurrence and development of peri-implantitis. |
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来源
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四川大学学报. 医学版
,2015,46(4):568-572 【核心库】
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关键词
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微生物多样性
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种植体周围炎
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高通量测序
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地址
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1.
西北民族大学口腔医学院, 口腔医学国家民委重点实验室, 兰州, 730030
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兰州大学口腔医学院, 兰州, 730000
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兰州石化总医院护理部, 兰州, 730060
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语种
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中文 |
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文献类型
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研究性论文 |
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ISSN
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1672-173X |
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学科
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微生物学;医药、卫生 |
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基金
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甘肃省兰州市科技局项目
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西北民族大学科研创新团队项目
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甘肃省自然科学基金
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西北民族大学中央高校基本科研业务费专项资金项目
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文献收藏号
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CSCD:5470732
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