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动物实验方法学和报告质量评估工具的横断面研究
Assessment Tools for Reporting Quality and Methodological Quality of Animal Experiments: A Cross-sectional Study

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牛军强 1   王亚楠 2   朱芊各 2   陈匡阳 2   张刘生 2   王亚平 2   苏旭 2   杨佳卉 2   秦倩 2   赵雅琴 2   郑建勋 2   黄成本 2   魏国强 2   陈朝虎 2   郑德秀 2   马彬 2 *  
文摘 目的全面收集国内外用于评估动物实验方法学和报告质量的工具/指南,比较其研发基础、适用范围和目的,为不同工具/指南的科学选择提供参考依据。方法计算机检索PubMed、EMbase、CNKI、VIP和WanFang Data,查找用于评估原始动物实验研究方法学和报告质量的工具/指南,检索时限截至2014年7月。提取各指南/工具的条目数、研发基础、基于的疾病模型,适用范围及评估重点等信息,对其行描述性分析。结果最终纳入32个研究,其中报告质量评估工具6个,方法学质量评估工具26个。各评估工具/指南的条目数在2~54之间,其中7个采用评分制对其质量进行评估。15个工具仅针对具体的疾病模型。19个适用于临床前药物研究,4个仅适用于环境毒理学方面的研究。结论至今虽已有多个评估工具用于评估动物实验的方法学质量,但SYRCLE动物实验偏倚风险评估工具是目前唯一专门针对动物实验内在真实性评估的工具。此外,对于报告工具,虽然ARRIVE指南和GSPC清单目前均非官方强制性的研究报告标准,但大多数学者公认其为撰写和发表动物实验的有效参考清单和写作指南。因此,推荐使用SYRCLE动物实验风险评估工具、ARRIVE指南和GSPC清单,以有效地提高动物实验设计、实施、报告以及对动物实验的鉴别能力、评估水平,促进动物实验的发展,促进科学成果充分利用和转化。
其他语种文摘 Objective To provide references for scientific selection of different tools/guidelines by comprehensively collecting international and national tools/guidelines for assessing reporting quality and methodological quality of animal experiments, comparing them in development foundation, application scope, and aims. Methods PubMed, EMbase, CNKI, VIP and WanFang Data were searched up to July 2014, to collect tools/guidelines for reporting quality and methodological quality of primary animal experiments. We extracted data from included guidelines/tools, including the number of items, development foundation, disease models, application scope, and assessment focus. Then descriptive analysis was conducted. Results A total of 32 studies were finally included, of which, 6 were for reporting quality and 26 for methodological quality. The item number of the included tools/guidelines ranged from 2 to 54. Seven tools/guidelines applied score system to assess methodological quality. Fifteen tools/guidelines were designed for specific disease models. Nineteen tools/guidelines were suitable for assessing preclinical drug studies, and 4 were designed to assess environmental toxicology research. Conclusion Although many tools for assessing methodological quality of animal experiments have been published so far, SYRCLE’s risk of bias tool is the only one that is used to assess internal validity of animal experiments at present. Besides, although the ARRIVE guidelines and GSPC are not official mandatory reporting criteria at present, they are acknowledged by many researchers as efficient reference checklists and writing guidelines for writing and publishing animal experiments. We recommend the application of SYRCLE’s risk of bias tool, ARRIVE guidelines and GSPC, in order to efficiently improve research design, implementation, reporting, differentiation, and evaluation of animal experiments, promote the development of animal experiments, and to promote full application and translation of scientific achievements.
来源 中国循证医学杂志 ,2015,15(2):223-229 【扩展库】
DOI 10.7507/1672-2531.20150038
关键词 动物实验 ; 方法学质量 ; 报告学质量 ; 指南/工具
地址

1. 兰州大学第一医院中西医结合疼痛科, 兰州, 730000  

2. 兰州大学循证医学中心,兰州大学基础医学院, 甘肃省循证医学与临床转化重点实验室, 兰州, 730000

语种 中文
文献类型 研究性论文
ISSN 1672-2531
学科 临床医学
基金 国家自然科学基金青年项目
文献收藏号 CSCD:5357939

参考文献 共 44 共3页

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引证文献 6

1 卢腾 施万细胞治疗大鼠创伤性脊髓损伤的Meta分析 中国循证医学杂志,2015,15(6):705-712
被引 0 次

2 方泽萍 兰州市动物实验研究人员对ARRIVE指南及GSPC知晓情况调查 中国循证医学杂志,2015,15(7):797-801
被引 2

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