帮助 关于我们

返回检索结果

纯钛表面涂层(Ca+Zn)/P 比值与成骨细胞生物活性的关系
Study of relationship between (Ca+Zn)/P of pure titanium surface coating and biological activity of osteoblasts

查看参考文献16篇

商亚微 1   张伟 2   何晶 1   王丹宁 1   钟鸣 3   赵宝红 1 *  
文摘 目的:研究纯钛表面不同(Ca+Zn)/P 比值对成骨细胞(MC3T3-E1)黏附、增殖和分化的影响,确定纯钛表面生物活性最佳的 Ca、P、Zn 含量。方法:在微弧氧化的电解液中加入一定浓度的 Ca、P 和5种不同浓度的 Zn(0、0.01、0.03、0.04和0.06 mol/L),使 Ca、P 元素的摩尔比值接近羟基磷灰石的比值。在纯钛材料表面制备5种不同(Ca+Zn)/P 比值的生物活性涂层,分别记为 S_0、S_1、S_2、S_3和 S_4组。应用 XPS 和 SEM 分析样品表面的元素组成、存在形式、(Ca+Zn)/P 比值以及表面形貌;应用 SEM、MTT 法和 ALP 活性测定法分析材料表面培养的MC3T3-E1细胞黏附、增殖和分化情况。采用 SPSS13.0软件包对数据进行统计学分析。结果:随着电解液中 Zn 浓度的增加,S_0~S_4组(Ca+Zn)/P 比值分别为2.94、2.64、2.71、2.87和2.72,其中,在 S_3组时达到最大,S_4组时降低。MC3T3-E1细胞在5组材料表面黏附、增殖和分化能力,依次为 S_3>S_4>S_2>S_1>S_0,各组之间差异具有显著性(P<0.05)。结论:应用微弧氧化法在纯钛表面制备的不同含量Ca、P、Zn 涂层,随着(Ca+Zn)/P比值的增大,成骨细胞的黏附、增殖和分化能力随之增强。其中,(Ca+Zn)/P比值为2.87时,细胞的生物活性最佳。
其他语种文摘 PURPOSE: To study osteoblast (MC3T3-E1 cells) adhesion, proliferation and differentiation activity on the surface of pure titanium with different (Ca+Zn)/P ratios,and determine Ca,P,Zn contents offering best biological activity on pure titanium surface. METHODS: In the micro-arc oxidation electrolyte, Ca, P with a certain concentration, and 5 different concentrations of Zn (0,0.01,0.03,0.04, and 0.06 mol/L) were added, which made the mole ratio of Ca and P element close to the ratio of hydroxyapatite. (Ca +Zn)/P ratio of bioactive coating on 5 different pure titanium material surfaces were prepared, which named S_0 and S_1, S_2, S_3 and S_4 group, respectively. The surface elements, existence form, (Ca + Zn)/P ratio and surface morphology were analyzed by XPS and SEM; MC3T3 -E1 cells adhesion, proliferation and differentiation cultured on the material surface were analyzed by SEM, MTT method and ALP activity assay. RESULTS: The data was analyzed with SPSS13.0 software package. With the increase of Zn content in the electrolyte, (Ca+Zn)/P ratios were 2.94, 2.64 2.71,2.87 and 2.72 in S_0 ~ S_4 group, respectively. (Ca+Zn)/P ratio was maximal in S 3 group and decreased in S_4 group. MC3T3-E1 cell adhesion, proliferation and differentiation capacity on material surface of 5 groups changed in the following order: S_3 > S_4 > S_2 > S_1 > S_0, with significant differences among 5 groups (P <0.05). CONCLUSIONS: Coating with different concentrations of Ca, P, Zn on pure titanium surface by micro-arc oxidation technique, the adhesion, proliferation and differentiation ability of osteoblast increase as (Ca+Zn)/P ratio increase, and cell biological activity is the best when the (Ca+Zn)/P ratio reached 2.87. Supported by Natural Science Foundation of Liaoning Province (20092093), Science and Technology Project of Liaoning Province (2013225049), and Science and Technology Project of Shenyang City (F11-264-1-25).
来源 上海口腔医学 ,2015,24(1):46-51 【扩展库】
关键词 微弧氧化 ; ; ; ; ; 成骨细胞
地址

1. 中国医科大学附属口腔医院种植中心,辽宁省口腔医学研究所口腔种植研究室,辽宁省口腔疾病转化医学研究中心, 辽宁, 沈阳, 110002  

2. 中国科学院金属腐蚀与防护研究所, 辽宁, 沈阳, 110016  

3. 中国医科大学口腔医学院中心实验室, 辽宁, 沈阳, 110002

语种 中文
文献类型 研究性论文
ISSN 1006-7248
学科 口腔科学
基金 辽宁省自然科学基金 ;  辽宁省科技计划项目 ;  辽宁省沈阳市科技计划项目
文献收藏号 CSCD:5357635

参考文献 共 16 共1页

1.  曾庆香. 种植体表面处理技术对骨结合影响研究进展. 中国实用口腔科杂志,2013,6(1):52-55 被引 1    
2.  Nagata M. Role of Zn in cellular Zn trafficking and mineralization in a murine osteoblast-like cell line. J Nutr Biochem,2011,22(2):172-178 被引 5    
3.  王丹宁. 纯钛表面应用微弧氧化法制备不同浓度锌涂层对成骨细胞生物学特性影响的研究. 口腔医学,2011,31(10):577-581 被引 4    
4.  Zhao B H. Effect of Zn content on cytoactivity and bacteriostasis of micro-arc oxidation coatings on pure titanium. Surf Coat Technol,2013,228(S1):428-432 被引 2    
5.  Ikeuchi M. Osteogenic differentiation of cultured rat and human bone marrow cells on the surface of Znreleasing calcium phosphate ceramics. J Biomed Mater Res A,2003,67(4):1115-1122 被引 2    
6.  Seo H J. Zn may increase bone formation through stimulating cell proliferation, alkaline phosphatase activity and collagen synthesis in osteoblastic MC3T3-E1 cells. Nutr Res Pract,2010,4(5):356-361 被引 18    
7.  余增丽. 锌对骨代谢影响的研究,2010 被引 1    
8.  Laschke M W. Injectable nanocrystalline hydroxyapatite paste for bone stitution: in vivo analysis of biocompatibility and vascularization. J Biomed Mater Res B Appl Biomater,2007,82(2):494-505 被引 5    
9.  Nishikawa T. Bone repair analysis in a novel biodegradable hydroxyapatite/collagen composite implanted in bone. Implant Dent,2005,14(3):252-260 被引 5    
10.  刘宝林. 口腔种植学,2011:24-25 被引 1    
11.  王磊. 钛表面微弧氧化膜的研究进展. 中国口腔种植学杂志,2006,11(1):48-50 被引 6    
12.  Gibson I R. Novel synthesis and characterization of an AB-type carbonate-substituted hydroxyapatite. J Biomed Mater Res,2002,59(4):697-708 被引 15    
13.  江源胜. 锌离子掺杂及电解液浓度变化对电化学沉积钙磷盐涂层的影响,2011 被引 1    
14.  Zou C. Preparation of nanosized betatricalcium phosphate particles with Zn substitution. J Mater Sci Mater Med,2008,19(3):1133-1136 被引 1    
15.  岑小波. 锌对大鼠成骨细胞内游离钙离子浓度和钙调蛋白活性的影响. 中国药理学与毒理学杂志,2001,15(1):43-46 被引 1    
16.  蒋百灵. 不同电导率溶液中镁合金微弧氧化陶瓷层的生长规律及耐蚀性. 稀有金属材料与工程,2005,34(3):393-396 被引 22    
引证文献 1

1 封伟 含锌钙磷涂层种植体成骨性能的动物实验 中华口腔医学杂志,2019,54(1):46-51
被引 0 次

显示所有1篇文献

论文科学数据集
PlumX Metrics
相关文献

 作者相关
 关键词相关
 参考文献相关

版权所有 ©2008 中国科学院文献情报中心 制作维护:中国科学院文献情报中心
地址:北京中关村北四环西路33号 邮政编码:100190 联系电话:(010)82627496 E-mail:cscd@mail.las.ac.cn 京ICP备05002861号-4 | 京公网安备11010802043238号