黄芪多糖改善老年大鼠的学习记忆水平及其机制研究
Study on effect of Astragali Radix polysaccharides in improving learning and memory functions in aged rats and its mechanism
查看参考文献20篇
文摘
|
该研究通过观察黄芪多糖对老年大鼠学习记忆的影响,试探究其改善学习记忆能力的机制。实验以自然衰老雌性 SD 大鼠为动物模型,随机分为老年对照组、老年黄芪多糖低、高剂量组 (50,150 mg · kg~(-1))和老年脑复康组 (阳性对照,560 mg·kg~(-1)),各组大鼠连续灌胃给药60 d,另设青年对照组。采用旷场和 Morris 水迷宫测试大鼠的学习记忆能力,蛋白免疫印迹法观察海马组织相关神经可塑性蛋白 N-甲基-D-天门冬氨酸受体 (NMDA 受体)、钙 / 钙调蛋白依赖的蛋白激酶 Ⅱ(CaMKⅡ)、蛋白激酶A(PKA)、cAMP 反应元件结合蛋白(CREB) 的磷酸化水平及脑源性神经营养调控因子 (BDNF) 的蛋白表达水平。研究发现与青年对照组比较,老年大鼠学习和记忆能力均显著下降(P < 0. 01),海马神经可塑性蛋白表达明显降低(P < 0. 01);与老年对照组比较,黄芪多糖能明显改善老年大鼠的学习记忆损伤,上调老年大鼠海马区神经可塑性相关蛋白表达水平。结果表明黄芪多糖可能通过改善神经可塑性相关蛋白水平的异常表达,进而改善老年大鼠学习记忆能力。 |
其他语种文摘
|
To observe the effect of Astragali Radix polysaccharides (APS) on the learning and memory functions of aged rats,in order to explore its mechanism for improving the learning and memory functions. Natural aging female SD rats were selected in the animal model and randomly divided into the control group,the APS low-dose group (50 mg · kg~(-1)),the APS high-dose group (150 mg·kg~(-1)) and the piracetam-treated group (560 mg·kg~(-1)). They were orally administered with the corresponding drugs for consecutively 60 days. Besides,a young control group was set. The learning and memory functions of the rats were tested by the open-field test and the Morris water maze task. The Western-blot method was used to observe the levels of relevant neural plasticity protein N-methyl-D-aspartate receptor (NMDA receptor) in hippocampus,calcium / calmodulin dependent protein kinase Ⅱ (CaMK Ⅱ),protein kinase (PKA),the phosphorylation level of CAMP response element binding protein (CREB) and the protein expression of brain derived neurotrophic factor(BDNF). In this study,the authors found that the learning and memory functions and the hippocampus neural plasticity protein expression of the aged rat group were much lower than that of the young control group (P <0. 01). Compared with the aged rat group,the APS group showed the significant improvement in the impaired learning and memory functions of aged rats and the up-regulation in the hippocampus neural plasticity protein expression. The results showed that APS may improve the learning and memory functions of aged rats by increasing the expressions of relevant neural plasticity proteins. |
来源
|
中国中药杂志
,2014,39(11):2071-2075 【核心库】
|
DOI
|
10.4268/cjcmm20141125
|
关键词
|
老年大鼠
;
黄芪多糖
;
神经可塑性
|
地址
|
1.
浙江医院中医内科, 浙江, 杭州, 310013
2.
中国科学院心理研究所, 中国科学院心理健康重点实验室, 北京, 100101
|
语种
|
中文 |
文献类型
|
研究性论文 |
ISSN
|
1001-5302 |
学科
|
中国医学 |
基金
|
中国科学院知识创新工程重要方向项目
;
浙江省中西医结合老年医学重点学科项目
|
文献收藏号
|
CSCD:5352269
|
参考文献 共
20
共1页
|
1.
Tohda C. Aβ (25-35)-induced memory impairment,axonal atrophy,and synaptic loss are ameliorated by MI,a metabolite of protopanaxadiol-type saponins.
Neuropsychopharmacology,2004,29(5):860
|
被引
18
次
|
|
|
|
2.
Shukitt-Hale B. Berry fruit supplementation and the aging brain.
J Agric Food Chem,2008,56(3):636
|
被引
3
次
|
|
|
|
3.
钟灵. 黄芪多糖抗衰老作用的实验研究.
中国应用生理学杂志,2013,29(4):350
|
被引
27
次
|
|
|
|
4.
Langui D. Animal models of neurodegenerative diseases.
Med Sci,2007,23(2):180
|
被引
2
次
|
|
|
|
5.
Pollak D D. Strain-dependent regulation of plasticity-related proteins in the mouse hippocampus.
Behav Brain Res,2005,165(2):240
|
被引
3
次
|
|
|
|
6.
Halbreich U. Possible acceleration of age effects on cognition following menopause.
J Psychiatr Res,1995,29(3):153
|
被引
3
次
|
|
|
|
7.
Hebda-Bauer E K. Female CREB-deficient mice show earlier age-related cognitive deficits than males.
Neuroscience,2007,150(2):260
|
被引
2
次
|
|
|
|
8.
Ahmed T. Plasticity-specific phosphorylation of CaMK Ⅱ,MAP-kinases and CREB during late-LTP in rat hippocampal slices in vitro.
Neuropharmacology,2005,49(4):477
|
被引
8
次
|
|
|
|
9.
Armbrecht H J. Effect of age on calcium-dependent proteins in hippocampus of senescence-accelerated mice.
Brain Res,1999,842(2):287
|
被引
3
次
|
|
|
|
10.
Lamprecht R. CREB:a message to remember.
Cell Mol Life Sci,1999,55(4):554
|
被引
2
次
|
|
|
|
11.
Gomez-Pinilla F. BDNF and learning:evidence that instrumental training promotes learning within the spinal cord by up-regulating BDNF expression.
Neuroscience,2007,148(4):893
|
被引
6
次
|
|
|
|
12.
刘丽琴. 人参皂苷对慢性应激抑郁模型大鼠行为学及HPA轴、BDNF的影响.
中国中药杂志,2011,36(10):1342
|
被引
11
次
|
|
|
|
13.
Donovan M H. Decreased adult hippocampal neurogenesis in the PDAPP mouse model of Alzheimer's disease.
J Comp Neurol,2006,495(1):70
|
被引
16
次
|
|
|
|
14.
周玥. 人参皂苷Rg_1在造血干/祖细胞连续移植中对抗细胞衰老的作用研究.
中国中药杂志,2013,38(17):2848
|
被引
7
次
|
|
|
|
15.
张先平. 当归多糖对小鼠衰老造血干细胞端粒、端粒酶及P53的影响.
中国中药杂志,2013,38(14):2354
|
被引
10
次
|
|
|
|
16.
张先平. 当归多糖抑制氧化损伤延缓造血干细胞衰老.
中国中药杂志,2013,38(3):407
|
被引
18
次
|
|
|
|
17.
陆兔林. 五味子多糖提取分离和药理作用研究进展.
中国中药杂志,2014,39(4):751
|
被引
20
次
|
|
|
|
18.
Cheng Y. Anti-amnestic and anti-aging effects of ginsenoside Rg1 and Rb1 and its mechanism of action.
Acta Pharm Sin,2005,26(2):143
|
被引
64
次
|
|
|
|
19.
赵海花. 人参皂甙对老龄大鼠Meynert核TrkB mRNA表达的影响.
中国组织化学与细胞化学杂志,2005,14(4):430
|
被引
3
次
|
|
|
|
20.
You Y H. Protective effects of Ganoderma lucidum polysaccharides peptide on injury of macrophages induced by reactive oxygen species.
Acta Pharm Sin,2002,23(9):787
|
被引
38
次
|
|
|
|
|