异育银鲫消化道微生物群落对恢复投喂的响应
EFFECTS OF REFEEDING ON THE DIVERSITY OF INTESTINAL MICROFLORA IN ALLOGYNOGENETIC CRUCIAL CARP (CARASSIUS AURATUS GIBELIO)
查看参考文献31篇
文摘
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以实验室养殖的异育银鲫(Carassius auratus gibelio)"中科3号"成鱼为对象,利用PCR-DGGE技术比较研究了其经饥饿与恢复投喂后消化道微生物多样性的变化。实验共检测到23条谱带,其中饥饿组、投喂组谱带数分别为18条和17条(共有谱带12条),投喂组大部分谱带亮度高于饥饿组。基于PCR-DGGE所得到的谱带进行的CA (Correspondence Analysis)分析和聚类都表明饥饿组与投喂组消化道微生物存在明显不同。结果表明,在充分饥饿处理后,恢复饲料投喂可导致消化道微生物种类减少及微生物平均密度的增加,同时对消化道微生态系统内微生物种类组成有显著影响。研究为进一步阐述消化道微生物功能基因奠定了基础,也为水产鱼类肠道菌群研究提供一些基础数据。 |
其他语种文摘
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In this study, we applied PCR-DGGE fingerprinting to investigate the effects of refeeding on the diversity of intestinal microflora in allogynogenetic crucian carp. Fishes were reared in a semi-recirculating system until maturation at Institute of Hydrobiology. After the 30-day starvation, fishes were divided into two groups (the hungry group and the feed group). Three fishes were blindly selected from the hungry group for the extraction of the microbial DNA with the traditional Phenol-chloroform method. In the meanwhile, fishes in the feed group resumed to be fed. Three fishes in the feed group were blindly selected for extraction of the microbial DNA after 25 days. The PCR-generated bacterial DNA products were separated using a polyacrylamide gel as it exhibited increasingly higher concentrations of chemical denaturant. Correspondence Analysis (CA) and clustering based on Jaccard index was used to analyze the similarity of the samples. The results showed that the feed group displayed less bands than the hungry group. There was significant difference in the deleted bands between the feed group (B1, B2, B5, B6, B7 and B23) and the hungry group (B11, B14, B16, B17 and B21), so we reasoned that the similarity of microflora between the two groups was low. The variance analysis showed that the variance within the feed group was larger than that within the hungry group. In conclusion, our results suggested that refeeding had strong effects on the diversity of intestinal microflora in allogynogenetic crucian carp. |
来源
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水生生物学报
,2014,38(5):854-859 【核心库】
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关键词
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异育银鲫
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消化道微生物群落
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PCR-DGGE
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肠道微生态系统
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地址
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中国科学院水生生物研究所, 中国科学院水生生物多样性与保护重点实验室, 武汉, 430072
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语种
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中文 |
文献类型
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研究性论文 |
ISSN
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1000-3207 |
学科
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水产、渔业 |
基金
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国家973计划
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国家自然科学基金
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文献收藏号
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CSCD:5251794
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参考文献 共
31
共2页
|
1.
Mueller K. The gut microbiota.
Science,2012,336:1245
|
被引
5
次
|
|
|
|
2.
Gomez G D. A review on the interactions between gut microbiota and innate immunity of fish.
FEMS Immunology and Medical Microbiology,2008,52:145-154
|
被引
31
次
|
|
|
|
3.
吕欣荣. 鱼类肠道菌群的研究现状.
江西水产科技,2008,2:12-18
|
被引
3
次
|
|
|
|
4.
王红宁. 鲤肠道正常菌群的研究.
水生生物学报,1994,18(4):354-359
|
被引
29
次
|
|
|
|
5.
John F R. Gnotobiotic zebrafish reveal evolutionarily conserved responses to the gut microbiota.
Proceedings of the National Academy of Sciences of the United States of America,2004,101(13):4596-4601
|
被引
47
次
|
|
|
|
6.
Bell T. Larger islands house more bacterial taxa.
Science,2005,308:1884
|
被引
21
次
|
|
|
|
7.
Romero J. 16S rDNA-Based analysis of dominant bacterial populations associated with early life stages of coho salmon (Oncorhynchus kisutch).
Microbial Ecology,2006,51:422-430
|
被引
18
次
|
|
|
|
8.
李学梅. 三种室内饲养鱼类肠道微生物群落PCR-DGGE指纹分析.
水生生物学报,2011,35(3):423-429
|
被引
16
次
|
|
|
|
9.
尹军霞. 鱼食性与肠道菌群关系的初步研究.
水产科学,2004,23(3):4-6
|
被引
15
次
|
|
|
|
10.
Margolis L. The effect of fasting on the bacterical flora of the intestine of fish.
Journal of the Fisheries Research Board of Canada,1953,10(2):95-104
|
被引
10
次
|
|
|
|
11.
Gui J F. Genetic basis and breeding application of clonal diversity and dual reproduction modes in polyploid Carassius auratus gibelio (review).
Science China Life Sciences,2010,53(4):409-415
|
被引
45
次
|
|
|
|
12.
Costello E K. The application of ecological theory toward an understanding of the human microbiome.
Science,2012,336:1255-1262
|
被引
24
次
|
|
|
|
13.
杨德君. 一种快速提取肠道微生物总DNA的方法.
中国微生态学杂志,2006,18(2):91-93
|
被引
9
次
|
|
|
|
14.
颜庆云. 滤食性鲢、鳙肠含物PCR-DGGE指纹分析.
水产学报,2009,33(6):972-979
|
被引
7
次
|
|
|
|
15.
Yan Q Y. Genetic diversity of plankton community as depicted by PCR-DGGE fingerprinting and its relation to morphological composition and environmental factors in Lake Donghu.
Microbial Ecology,2007,54(2):290-297
|
被引
29
次
|
|
|
|
16.
Li X M. Host species as a strong determinant of the intestinal microbiota of fish larvae.
The Journal of Microbiology,2012,50:29-37
|
被引
13
次
|
|
|
|
17.
余育和. DNA指纹分析技术在群落级生命系统应用的可能性.
水生生物学报,2004,28(5):457-463
|
被引
22
次
|
|
|
|
18.
马正学. 甘肃小陇山桃花沟森林公园土壤纤毛虫的群落特征.
生态学杂志,2008,27(2):208-212
|
被引
8
次
|
|
|
|
19.
冯雪. 草鱼和银鲫肠道产消化酶细菌的研究.
淡水渔业,2008,38(3):51-57
|
被引
18
次
|
|
|
|
20.
易中华. 消化道微生物对宿主动物氨基酸的自平衡调节作用.
中国畜牧杂志,2005,41(9):64-66
|
被引
1
次
|
|
|
|
|