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Characterization of human αβTCR repertoire and discovery of D-D fusion in TCRβ chains

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Liu Peipei 1   Di Liu 2   Xi Yang 1   Jing Gao 1   Yan Chen 1   Xue Xiao 1   Fei Liu 1   Jing Zou 1   Jun Wu 1   Ma Juncai 2   Zhao Fangqing 3   Zhou Xuyu 1 *   Gao George F 1 *   Zhu Baoli 1 *  
文摘 The characterization of the human T-cell receptor (TCR) repertoire has made remarkable progress, with most of the work focusing on the TCRβ chains. Here, we analyzed the diversity and complexity of both the TCRα and TCRβ repertoires of three healthy donors. We found that the diversity of the TCRα repertoire is higher than that of the TCRβ repertoire, whereas the usages of the V and J genes tended to be preferential with similar TRAV and TRAJ patterns in all three donors. The V-J pairings, like the V and J gene usages, were slightly preferential. We also found that the TRDV1 gene rearranges with the majority of TRAJ genes, suggesting that TRDV1 is a shared TRAV/DV gene (TRAV42/DV1). Moreover, we uncovered the presence of tandem TRBD (TRB D gene) usage in ～2% of the productive human TCRβ CDR3 sequences.
来源 Protein & Cell ,2014,5(8):603-615 【核心库】
DOI 10.1007/s13238-014-0060-1
关键词 TCR repertoire ; next-generation sequencing ; V/J usage ; V-J pairing ; CDR3 ; D-D fusion
地址

1. Institute of Microbiology, Chinese Academy of Sciences, CAS Key Laboratory of Pathogenic Microbiology and Immunology, Beijing, 100101  

2. Network Information Center, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101  

3. Research Network of Immunity and Health (RNIH), Beijing Institutes of Life Science, Chinese Academy of Sciences, Beijing, 100101

语种 英文
文献类型 研究性论文
ISSN 1674-800X
基金 国家自然科学基金 ;  国家973计划 ;  国家自然科学基金 ;  G.F.G.is a leading principal investigator of the NSFC Innovative Research Group
文献收藏号 CSCD:5242654

参考文献 共 46 共3页

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