鳙Sox基因克隆及序列进化分析
CLONING AND SEQUENCE EVOLUTION ANALYSIS OF SOX GENES IN BIGHEAD CARP (ARISTICHTHYS NOBILIS)
查看参考文献26篇
文摘
|
利用简并引物SoxN和Sox9在鳙基因组DNA中进行PCR扩增和产物克隆测序, 并对序列进行同源性比较和系统进化分析。结果表明本文鉴定出鳙15个Sox基因HMG盒序列, 分别属于SoxB、SoxC和SoxE组, 依据斑马鱼同源基因将其分别命名为Sox1a、Sox1b、Sox2、Sox3、Sox4a、Sox4b、Sox9a、Sox9b、Sox10、Sox11b、Sox12、Sox14a、Sox14b、Sox19和Sox21a。基于鳙和斑马鱼 Sox1、Sox4和Sox9基因核苷酸序列构建的系统进化树显示这3个Sox基因的复制时间发生在鳙和斑马鱼的分化之前, 结果支持了鱼类特异的基因组复制假说。以Sox1a、Sox1b和Sox4基因为分子钟标记构建系统进化树探讨鳙和斑马鱼的分化时间, 结果显示, 同属于鲤科鱼类的鳙(鲤亚科)和斑马鱼(鱼丹亚科)在原始的鱼丹亚科鱼类中存在一个共同祖先, 大约出现在63.7百万年前。研究结果为进一步研究鱼类Sox基因复制和基因组进化等问题提供了重要参考资料。 |
其他语种文摘
|
To amplify Sox genes from bighead carp genome, two pairs of degenerate primers (SoxN and Sox9) were designed for PCR amplification that were utilized for sequencing and analysis of sequence homology and phylogenetic relationships. The results showed that 15 distinct Sox genes encoding the HMG domains were identified in bighead carp, which were assigned to group B, C and E. According to their homology to orthologs of zebrafish, 15 Sox genes were designated as Sox1a, Sox1b, Sox2, Sox3, Sox4a, Sox4b, Sox9a, Sox9b, Sox10, Sox11b, Sox12, Sox14a, Sox14b, Sox19 and Sox21a, respectively. Phylogenetic tree of Sox1, Sox4 and Sox9 nucleotide sequences indicated that the duplication of these three Sox genes occurred before the divergence of bighead carp and zebrafish, and this observation supported the "fish-specific whole-genome duplication" theory. Using Sox1a, Sox1b and Sox4 as molecular clock markers in phylogenetic analysis, the estimation of the divergence time between bighead carp and zebrafish demonstrated that in original Danioninae, a common ancestor appeared approximately 63.7 million years ago for bighead carp and zebrafish, both of which belong to Cyprinidae. This study would provide important information for further studies of Sox gene replication and genome evolution in fish. |
来源
|
水生生物学报
,2014,38(4):664-668 【核心库】
|
关键词
|
鳙
;
Sox基因
;
基因组加倍
;
系统进化树
;
分化时间
|
地址
|
中国科学院水生生物研究所, 武汉, 430072
|
语种
|
中文 |
文献类型
|
研究性论文 |
ISSN
|
1000-3207 |
学科
|
分子生物学 |
基金
|
公益性(农业)行业科研项目
;
国家自然科学基金项目
|
文献收藏号
|
CSCD:5202452
|
参考文献 共
26
共2页
|
1.
Rehberg S. Sox10 is an active nucleo-cytoplasmic shuttle protein and shuttling is crucial for Sox10-mediated transactivation.
Molecular and Cellular Biology,2002,22:5826-5834
|
被引
6
次
|
|
|
|
2.
Zanaria E. An unusual member of the nuclear hormone receptor superfamily responsible for X-linked adrenal hypoplasia congenita.
Nature,1994,372:635-641
|
被引
32
次
|
|
|
|
3.
Uwanogho D. Embryonic expression of the chicken Sox2, Sox3 and Sox11 genes suggests an interactive role in neuronal development.
Mechanisms of Development,1995,49(1):23-36
|
被引
5
次
|
|
|
|
4.
Collignon J. A comparison of the properties of Sox3 with Sry and two related genes Sox1 and Sox2.
Development,1996,122(2):509
|
被引
16
次
|
|
|
|
5.
Cheung M. Roles of Sox4 in central nervous system development.
Molecular Brain Research,2000,79(1):180-191
|
被引
9
次
|
|
|
|
6.
Bowles J. Phylogeny of the Sox family of developmental transcription factors based on sequence and structural indicators.
Development Biology,2000,227(2):239-255
|
被引
67
次
|
|
|
|
7.
Okuda Y. Comparative genomic and expression analysis of group B1 sox genes in zebrafish indicates their diversification during vertebrate evolution.
Developmental Dynamics,2006,235(3):811-825
|
被引
6
次
|
|
|
|
8.
Vandepoele K. Major events in the genome evolution of vertebrates: paranome age and size differ considerably between ray-finned fishes and land vertebrates.
Proceedings of the National Academy of the Sciences ot the United States of America,2004,101(6):1638-1643
|
被引
20
次
|
|
|
|
9.
Zheng J. Isolation and sequence analysis of Sox genes in the red crucian carp (Carassius carassius, red variety).
Russian Journal of Genetics,2008,44(11):1325-1330
|
被引
2
次
|
|
|
|
10.
Zhong L. Sox genes in grass carp (Ctenopharyngodon idella) with their implications for genome duplication and evolution.
Genetics Selection Evolution,2006,38:673-687
|
被引
4
次
|
|
|
|
11.
Nei M. Purifying selection and birth-and-death evolution in the ubiquitin gene family.
Proceeding of the National Academy of the Sciences,2000,97(20):10866-10871
|
被引
3
次
|
|
|
|
12.
Nei M.
Molecular Evolution and Phylogenetics,2000:203-206
|
被引
1
次
|
|
|
|
13.
韩亚伟. 鲤科鱼类系统进化过程中SINEs的插入事件.
自然科学进展,2007,17(10):1345-1351
|
被引
2
次
|
|
|
|
14.
陈宜瑜. 珠江的鱼类区系及其动物地理区划的讨论.
水生生物学报,1986,10(3):228-236
|
被引
41
次
|
|
|
|
15.
Taggart J B. A simplified protocol for routine total DNA isolation from salmonid fishes.
Journal of Fish Biology,1992,40(6):963-965
|
被引
15
次
|
|
|
|
16.
Galay-Burgos M. Analysis of the Sox gene family in the European sea bass (Dicentrarchus labrax).
Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology,2004,137(2):279-284
|
被引
8
次
|
|
|
|
17.
Thompson J D. The Clustal_X Windows interface: Flexible strategies for multiple sequences alignment aided by quality analysis Tools.
Nucleic Acids Research,1997,25(24):4876-4882
|
被引
2384
次
|
|
|
|
18.
Tamura K. Mega 4: Molecular evolutionary genetics analysis (Mega) software version 4.0.
Molecular Biology and Evolution,2007,24(8):1596-1599
|
被引
1999
次
|
|
|
|
19.
Hughes A L.
Adaptive Evolution of Genes and Genomes,1999:15-37
|
被引
1
次
|
|
|
|
20.
Kumazawa Y.
The Biology of Biodiversity,1999:35-52
|
被引
2
次
|
|
|
|
|