温度对铜绿微囊藻细胞浮力的调控机制
Effects of temperature on the buoyancy of Microcystis aeruginosa
查看参考文献35篇
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文摘
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为了探讨温度对铜绿微囊藻细胞浮力的调控机制,将铜绿微囊藻分别置于10、15、20、25、30℃条件下,利用改良的percoll密度梯度离心法测得细胞密度,计算出细胞浮力大小,同时测量细胞内比生长速率、光合活性、NAD(P)H依赖型氧化还原脱氢酶活性、糖含量、蛋白含量和漂浮细胞百分比.结果显示,在实验温度范围内(10~30℃),随着温度升高,铜绿微囊藻的漂浮细胞百分率增加,浮力变大;同时,细胞内糖含量减少,蛋白含量增加,光合作用和NAD(P)H依赖型氧化还原脱氢酶活性增强.10、15℃条件培养7d,细胞的密度分别增加了2.7%和1.3%,糖含量分别增加了95.3%和65.5%,漂浮细胞百分比分别降低了23.8%和18.3,NAD(P)H依赖型氧化还原脱氢酶活性分别降低了23.8%和18.3%;而20、25和30℃条件培养7d,细胞的密度则分别减少了2.8%、3.8%和3.2%,糖含量分别减少了8.5%、2.9%和19.4%,漂浮细胞百分比分别增加了0%、7%和8.5%,NAD(P)H依赖型氧化还原脱氢酶活性分别增加了0%、7%和8.5%.研究结果显示:温度主要通过影响铜绿微囊藻细胞的光合速率、生长代谢速率和伪空胞含量来改变细胞密度实现浮力调节.低温下糖含量增加是浮力消失的主要原因,细胞漂浮与沉降的温度阈值在15~20℃之间. |
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其他语种文摘
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Microcystis aeruginosa were cultured in different temperature: 10℃, 15℃, 20℃, 25℃, 30℃ to screen the effects of temperature on buoyancy regulation. Cell density was measured by percoll density gradient centrifugation and cell buoyancy was calculated by the buoyancy formula. Simultaneously, biomass, specific growth rate, the photosynthetic activity, the activity of NAD (P)H dependent oxidoreductase, intracellular carbohydrate, protein content, the percentage of floating cells were measured. With the increasing of the experimental temperature (10℃-30℃), buoyancy, the percentage of floating cells, intracellular protein content, photosynthesis rate and the activity of NAD (P)H dependent oxidoreductase increased and the intracellular carbohydrate content decreased. After 7days cultured at 10, 15℃, cell density were increased by 2.7% and 1.3%, respectively; carbohydrate content were increased by 95.3% and 65.5%, respectively; the percentage of floating cells were reduced by 23.8% and 18.3, respectively; the activity of NAD (P)H dependent oxidoreductase were reduced by 23.8 % and 18.3% respectively. After 7days cultured at 20, 25, 30℃, cell density were reduced by 2.8%, 3.8% and 3.2%, respectively; carbohydrate content were reduced by 8.5%, 2.9% and 19.4%, respectively; the percentage of floating cells were increased by 0%, 7% and 8.5%, respectively; the activity of NAD (P)H dependent oxidoreductase were increased by 0%, 7% and 8.5%, respectively. It was concluded that cell growth rate, photosynthetic rate, metabolic rate were the function of temperature in the buoyancy regulation of Microcystis aeruginosa. The main reason for the buoyancy loss is the accumulation of carbohydrate, the temperature threshold value for the buoyancy is between 15~20℃. |
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来源
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中国环境科学
,2014,34(7):1847-1854 【核心库】
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关键词
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温度
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细胞浮力
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调控机制
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铜绿微囊藻
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地址
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中国科学院水生生物研究所, 淡水生态与生物技术国家重点实验室, 湖北, 武汉, 430072
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语种
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中文 |
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文献类型
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研究性论文 |
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ISSN
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1000-6923 |
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学科
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环境科学基础理论 |
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基金
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国家自然科学基金项目
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国家973计划
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文献收藏号
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CSCD:5199268
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