马铃薯蛋白的分离及氨基酸组成分析
Isolation and Amino Acid Analysis of Potato Protein
查看参考文献25篇
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文摘
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为了从马铃薯淀粉加工分离汁水中回收具有天然活性的蛋白,以Amberlite XAD7HP树脂作为吸附剂对扩张床吸附进行研究,并分析回收蛋白的氨基酸组成。结果表明:XAD7HP可以实现Patatin蛋白和蛋白酶抑制剂的分离,分离到的马铃薯蛋白酶抑制剂的胰蛋白酶抑制活力为410 mg/g。回收的马铃薯蛋白酶抑制剂Ser、Leu、Glu的含量分别为13.2、9.14、8.14 g/100 g,且必需氨基酸占总氨基酸的40.80%,疏水氨基酸Ile、Leu、Val、Phe和Tyr的含量也比较高。 |
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其他语种文摘
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Bioactive proteins were recovered from a liquid byp roduct containing roughly 1.5% protein, with approximately 50% being protease inhibitors by expanded bed adsorption using macroporous resin Amberlite XAD7HP. The recovered proteins were analyzed for amino acid composition. The results indicated that XAD7HP allowed the separation between protease inhibitors and patatin. The protease inhibitors exhibited an activity of 410 mg/g against trypsin and contained Ser, Leu and Glu at 3.2, 9.14 and 8.14 g/100 g, respectively, with the ratio of essential to total amino acids being 40.80%. Similarly, hydrophobic amino acids, Ile, Leu, Val, Phe and Tyr, were present in relatively higher quantities in these protease inhibitors. |
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来源
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食品科学
,2014,35(9):53-56 【核心库】
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关键词
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马铃薯
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天然蛋白
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蛋白酶抑制剂
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氨基酸组成
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地址
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中国科学院兰州化学物理研究所,环境材料与生态化学研究发展中心, 甘肃, 兰州, 730000
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语种
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中文 |
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文献类型
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研究性论文 |
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ISSN
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1002-6630 |
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学科
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轻工业、手工业、生活服务业 |
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基金
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国家现代农业(马铃薯)产业技术体系建设专项
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国家自然科学基金青年科学基金项目
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文献收藏号
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CSCD:5167878
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参考文献 共
25
共2页
|
|
1.
Pots A M. The effect of storage of whole potatoes of three cultivars on the patatin and protease inhibitor content: a study using capillary electrophoresis and MA LDITOF mass spectrometry.
Journal of the Science of Food and Agriculture,1999,79(12):1557-1564
|
被引
5
次
|
|
|
|
|
2.
Bauw G. Patatins, Kunitz protease inhibitors and other major proteins in tuber of potato cv. Kuras.
FEBS Journal,2006,273(15):3569-3584
|
被引
4
次
|
|
|
|
|
3.
Beekwilder J. Characterization of potato proteinase inhibitor II reactive site mutants.
European Journal of Biochemistry,2000,267:1975-1984
|
被引
4
次
|
|
|
|
|
4.
Knorr D. Potato protein concentrates: the influence of various methods of recovery upon yield, compositional and functional characteristics.
Journal of Food Processing and Preservation,1977,1(3):235-247
|
被引
4
次
|
|
|
|
|
5.
Parfenov I A. Fragment of the gene encoding chymotrypsin and trypsin inhibitor protein of potato tubers.
Applied Biochemistry and Microbiology,2011,47:361-365
|
被引
1
次
|
|
|
|
|
6.
Park Y. Kunitz-type serine protease inhibitor from potato (Solanum tuberosum L. cv. Jopung).
Journal of Agricultural and Food Chemistry,2005,53:6491-6496
|
被引
3
次
|
|
|
|
|
7.
Kim M H. Purification and characterization of a heat-stable serine protease inhibitor from the tubers of new potato variety "Golden Valley".
Biochemical and Biophysical Research Communications,2006,346:681-686
|
被引
6
次
|
|
|
|
|
8.
Singh J.
Advances in potato chemistry and technology,2009
|
被引
2
次
|
|
|
|
|
9.
Hu J.
Potato proteinase inhibitor II exhibits activity in elevating fasting plasma cholecystokinin concentrations: United States, 20060204567,2006
|
被引
1
次
|
|
|
|
|
10.
Wang X. Murine model of ferric chlorideinduced vena cava thrombosis: evidence for effect of p otato carboxypeptidase inhibitor.
Journal of Thrombosis and Haemos tasis,2006,4(2):403-410
|
被引
5
次
|
|
|
|
|
11.
Blanco-Aparicio C. Potato carboxypeptidase inhibitor, a T-knot protein, is an epidermal growth factor antagonist that inhibits tumor cell growth.
Journal of Biological Chemistry,1998,273:12370-12377
|
被引
3
次
|
|
|
|
|
12.
Huang C. Proteinase inhibitors I and II from potatoes specifically block UV-induced activator protein-1 activation through a pathway that is independent of extracellular signal-regulated kinases, c-Jun N-terminal kinases, and P38 kinase.
Proceedings of the National Academy of Sciences of the United States of America,1997,94(22):11957-11962
|
被引
1
次
|
|
|
|
|
13.
Ralla K. Separation of patatins and protease inhibitors from potato fruit juice with clay minerals as cation exchangers.
Journal of Separation Science,2012,35(13):1596-1602
|
被引
3
次
|
|
|
|
|
14.
STRAETKVERN K O. Recovery of native potato protein comparing expanded bed adsorption and ultrafiltration.
Food Bioprocess Technology,2012,5(5):1939-1949
|
被引
5
次
|
|
|
|
|
15.
齐斌. 马铃薯分离蛋白的提取工艺.
食品科学,2010,31(22):297-300
|
被引
5
次
|
|
|
|
|
16.
崔竹梅. 马铃薯蛋白组分的分离提取和功能性质研究.
食品科学,2011,32(3):76-80
|
被引
5
次
|
|
|
|
|
17.
Chase H A. Expanded-bed adsorption of proteins using ion-exchangers.
Separation Science and Technology,1992,27(14):2021-2039
|
被引
6
次
|
|
|
|
|
18.
Mccormick D K. Expanded bed adsorption-the first new unit operation in decades.
Nature Biotechnology,1993,11:1059-1060
|
被引
1
次
|
|
|
|
|
19.
Giuseppin M L F.
Native potato protein isolates:WO Patent, WO 2008/069 650 A1,2008
|
被引
1
次
|
|
|
|
|
20.
STRAETKVERN K O. Expanded bed adsorption for recovery of patatin from crude potato juice.
Bioseparation,1999,7(6):333-345
|
被引
2
次
|
|
|
|
|
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