微囊藻毒素-LR对小鼠肠道消化酶的影响
EFFECTS OF MICROCYSTIN-LR ON THE DIGESTIVE ENZYME ACTIVITY OF INTESTINAL TRACT IN BALC/C MICE
查看参考文献36篇
|
文摘
|
微囊藻毒素-LR(MCLR)是一类对动物和人类健康影响很大的蓝藻毒素。已有调查认为MCLR能导致某些胃肠道疾病, 相关实验室资料也证实MCLR能在肠道积累并引起肠道损伤。研究对小鼠连续腹腔染毒MCLR 28d, 观察肠道病理水平及超微结构的变化, 并测定肠黏膜刷状缘膜酶活性。结果显示, 小肠绒毛受损较严重, 绒毛数量减少、部分脱落至肠腔, 固有层及黏膜下层水肿、充血; 电镜观察发现肠细胞细胞质电子密度降低, 伴有线粒体肿胀、细胞核变形现象; 肠黏膜二糖酶(蔗糖酶、麦芽糖酶、乳糖酶)、碱性磷酸酶及γ-谷氨酰转移酶活性均呈下降趋势。在亚慢性MCLR染毒条件下, 肠道的消化功能可能受到抑制, 进而导致机体对营养物质吸收不良。研究对理解蓝藻毒素引起的胃肠道不适(如恶心、呕吐、腹痛、腹泻)提供了新的证据。 |
|
其他语种文摘
|
Microcystin-LR has been known to harm the health in both humans and animals. Recently there has been an increase in reports about gastrointestinal ailments resulting from the ingestion of MCLR. Some data also indicated that MCLR could accumulate in the intestinal tract and cause injuries. In this study, we intraperitoneally injected mice with MCLR for 28 days, and observed the pathophysiological and ultrastructural changes. We also examined the activities of intestinal mucosal brush border membrane enzymes. Severe injuries were found in villi, such as a decrease in villi number and the exfoliation of villi into gut cavity. The cells of lamina propria and submucosa showed swell and hyperemia. In the ultrathin sections, loss of density in the cytoplasm associated with swollen mitochondria as well as nuclei deformation was observed. The activities of intestinal disaccharidase (sucrase, maltase, lactase), alkaline phosphatase, and γ-glutamyl transferase decreased after the MCLR exposure. Based on these results, we reasoned that the digestion capacity of small intestine might be inhibited after subchronic exposure of MCLR, which led to malabsorption. Our study suggested new cellular mechanisms that explain gastrointestinal sickness, such as nausea, vomiting and diarrhea, induced by cyanobacterial toxins. |
|
来源
|
水生生物学报
,2014,38(3):533-539 【核心库】
|
|
关键词
|
微囊藻毒素
;
肠道
;
酶活
;
超微结构
|
|
地址
|
1.
华中农业大学水产学院, 武汉, 430074
2.
中国科学院水生生物研究所, 武汉, 430072
|
|
语种
|
中文 |
|
文献类型
|
研究性论文 |
|
ISSN
|
1000-3207 |
|
学科
|
环境污染及其防治 |
|
基金
|
国家自然科学基金
|
|
文献收藏号
|
CSCD:5146590
|
参考文献 共
36
共2页
|
|
1.
Carmichael W W. The toxins of cyanobacteria.
Scientific American,1994,270:64-72
|
被引
71
次
|
|
|
|
|
2.
李效宇. 微囊藻细胞抽提液对小鼠血液的亚慢性毒性.
水生生物学报,2008,12(6):811-817
|
被引
2
次
|
|
|
|
|
3.
吴幸强. 微囊藻毒素在滇池鱼体内的积累水平及分布特征.
水生生物学报,2010,34(2):388-393
|
被引
15
次
|
|
|
|
|
4.
Ufelmann H. Human and rat hepatocyte toxicity and protein phosphatase 1 and 2A inhibitory activity of naturally occurring desmethyl-microcystins and nodularins.
Toxicology,2012,293(1/3):59-67
|
被引
6
次
|
|
|
|
|
5.
MacKintosh C. Cyanobacterial microcystin-LR is a potent and specific inhibitor of protein phosphatases 1 and 2A from both mammals and higher plants.
FEBS Letters,1990,264(2):187-192
|
被引
56
次
|
|
|
|
|
6.
Eriksson J E. Hepatocyte deformation induced by cyanobacterial toxins reflects inhibition of protein phosphatases.
Biochemical and Biophysical Research Communications,1990,173(3):1347-1353
|
被引
8
次
|
|
|
|
|
7.
Ohta T. Hyperphosphorylation of cytokeratins 8 and 18 by microcystin-LR, a new liver tumor promoter, in primary cultured rat hepatocytes.
Carcinogenesis,1992,13(12):2443-2447
|
被引
2
次
|
|
|
|
|
8.
Ding W X. Critical role of reactive oxygen species and mitochondrial permeability transition in microcystin-induced rapid apoptosis in rat hepatocytes.
Hepatology,2000,32(3):547-555
|
被引
33
次
|
|
|
|
|
9.
Gehringer M M. An investigation into the detoxification of microcystin-LR by the glutathione pathway in Balb/c mice.
The International Journal of Biochemistry & Cell Biology,2004,36(5):931-941
|
被引
23
次
|
|
|
|
|
10.
el Saadi O E. Murray River water, raised cyanobacterial cell counts, and gastrointestinal and dermatological symptoms.
The Medical Journal of Australia,1995,162(3):122-125
|
被引
3
次
|
|
|
|
|
11.
Ito E. First report on the distribution of orally administered microcystin-LR in mouse tissue using an immunostaining method.
Toxicon,2000,38(1):37-48
|
被引
7
次
|
|
|
|
|
12.
Ernst B. Oral toxicity of the microcystin-containing cyanobacterium Planktothrix rubescens in European whitefish (Coregonus lavaretus).
Aquatic Toxicology,2006,79(1):31-40
|
被引
3
次
|
|
|
|
|
13.
Djediat C. Oral toxicity of extracts of the microcystin-containing cyanobacterium Planktothrix agardhii to the medaka fish (Oryzias latipes).
Toxicon,2011,58(1):112-122
|
被引
2
次
|
|
|
|
|
14.
Ferreira M F N. Histopathological effects of [D-Leu1]Microcystin-LR variants on liver, skeletal muscle and intestinal tract of Hypophthalmichthys molitrix (Valenciennes, 1844).
Toxicon,2010,55(7):1255-1262
|
被引
1
次
|
|
|
|
|
15.
Ito E. Intratracheal administration of microcystin-LR, and its distribution.
Toxicon,2001,39(2/3):265-271
|
被引
2
次
|
|
|
|
|
16.
Botha N. The role of microcystin-LR in the induction of apoptosis and oxidative stress in CaCO2 cells.
Toxicon,2004,43(1):85-92
|
被引
8
次
|
|
|
|
|
17.
Toofanian F. Fetal development and distribution of intestinal alkaline phosphatase in rabbit.
Research in Veterinary Science,1982,32(3):303-305
|
被引
3
次
|
|
|
|
|
18.
Uauy R. Effect of dietary nucleosides on growth and maturation of the developing gut in the rat.
Journal of Pediatric Gastroenterology and Nutrition,1990,10(4):497-503
|
被引
18
次
|
|
|
|
|
19.
McCracken V J. The gastrointestinal ecosystem: a precarious alliance among epithelium, immunity and microbiota.
Cellular Microbiology,2001,3(1):1-11
|
被引
5
次
|
|
|
|
|
20.
Bates J M. Intestinal alkaline phosphatase detoxifies lipopolysaccharide and prevents inflammation in zebrafish in response to the gut microbiota.
Cell Host & Microbe,2007,2(6):371-382
|
被引
26
次
|
|
|
|
|
了解气象卫星更多信息，请访问