静电纺丝素/胶原/聚(左旋乳酸-己内酯)三维纳米纤维支架及细胞相容性研究
Preparation and cytocompatibility of SF/COL/PLCL 3-dimensional nanofibers scaffolds by electrospinning
查看参考文献12篇
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文摘
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采用静电纺丝技术,以丝素(SF)胶原(COL)混合聚(左旋乳酸-己内酯)(PLCL)制备不同配比的三维纳米纤维支架材料,表征其理化性能并检测细胞相容性,旨为牙周组织工程提供一种新型支架材料。采用扫描电镜(SEM),傅里叶红外光谱(FTIR),接触角等方法表征其理化性能;接种牙周膜干细胞(PDLSCs)检测其细胞生物相容性。结果表明:SF/COL/PLCL纤维形貌良好,直径随PLCL含量的增加而变大;SF/COL对PLCL的结构几乎没有影响,但PLCL会破坏丝素胶原之间形成的氢键,使以β折叠结构为主的SF变为以无规卷曲和α螺旋结构为主;当SF/COL与PLCL质量比为50:50时,三维纳米纤维支架材料的亲水性能良好,牙周膜干细胞(PDLSCs)在纤维表面粘附稳定,增殖活性良好,表明该支架材料细胞生物相容性好,在生物医学领域具有很大的应用潜力。 |
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其他语种文摘
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The different weight ratio of silk fibroin/collagen/PLCL 3-dimensional nanofiber scaffold were prepared using electrospinning technique and to fabricate a kind of nanofiber scaffold for periodontal tissue engineering.The fibers were characterized by combined techniques of scanning electron microscopy(SEM),FTIR and water contact angle.The fibers were inoculated with periodontal ligament stem cells(PDLSCs).The cell adhesion and proliferation were evaluated by SEM and MTT.The results indicated that:the form of scaffold materials were smoothed and uniformed.SF/COL almost made no difference to the polymer,but PLCL showed some impact on SF which resulted the conformation changing fromβ- sheet to random coil andα-helix;3-dimensional nanofiber scaffold displaied good hydrophilic property when SF/COL and PLCL weight ratio was 50:50.In addtion,the cells grew well on the surface of this ratio of SF/COL and PLCL,and had the best adhesionand and proliferation.The nanofiber scaffold might be a potential candidate for periodontal tissue engineering. |
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来源
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化工新型材料
,2014,42(4):84-87 【核心库】
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关键词
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静电纺
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丝素
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胶原
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聚(左旋乳酸-己内酯)
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细胞相容性
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地址
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兰州大学口腔医学院, 兰州, 730000
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语种
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中文 |
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文献类型
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研究性论文 |
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ISSN
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1006-3536 |
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学科
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化学工业 |
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基金
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国家自然科学基金青年科学基金项目
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教育部中央高校基本科研业务费项目面上项目
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文献收藏号
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CSCD:5134929
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