蓖麻DNA糖基化酶的序列与基因表达分析
Sequence and expression analysis of DNA glycosylase in castor bean (Ricinus communis L.)
查看参考文献29篇
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文摘
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为揭示DNA糖基化酶在调节蓖麻生长发育和影响基因印记过程中的分子机理,本研究基于蓖麻全基因组、利用生物信息学的方法结合转录组数据和RT-PCR半定量表达分析,鉴别蓖麻DNA糖基化酶氨基酸序列的结构特征、系统发生和表达形式。结果显示:三种DNA糖基化酶(DME、ROS和DML)氨基酸均为不稳定氨基酸,具有典型的与DNA结合和碱基切除修复有关的保守结构域;系统进化分析发现三种DNA糖基化酶在植物中同源性强,而且是独立演化的结果;在不同组织的表达上,蓖麻的DME和ROS基因具有相似的表达形式,即在根、茎和叶中不表达,在胚乳中表达最高,而DML在各个组织中都未检测到基因的表达。 |
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其他语种文摘
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To reveal the potential function of DNA glycosylases mediating DNA demethylation leading to gene imprinting in the growth and development of castor bean (Ricinus communis), the putative genes encoding DNA glycosylases were identified and characterized based on castor bean genome reference data. 3 genes (RcDME, RcROS and RcDML) encoding DNA glycosylases were identified. Their structures, conserved domains, and subcellular localization were characterized. Gene expression was inspected based on the high-throughput transcriptional sequencing data and RT-PCR. Results showed that the amino acids coded by the 3 genes were unstable ones, and the sequences contained typical DNA-binding conserved domains and domains related to base excision repair. Phylogenetically, DME, ROS and DML were homologous and evolved independently in plants. Both RcDME and RcROS exhibited a similar expression pattern among different tissues with the highest expression in endosperm, while the expression level of RcDML was not detected in all tested tissues. |
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来源
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中国油料作物学报
,2014,36(2):181-188 【核心库】
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关键词
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蓖麻
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DNA糖基化酶
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脱甲基化
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表观调控
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地址
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1.
中国科学院大学, 中国科学院西双版纳热带植物园热带植物资源开放实验室, 北京, 100049
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中国科学院昆明植物研究所, 云南, 昆明, 650201
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语种
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中文 |
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文献类型
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研究性论文 |
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ISSN
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1007-9084 |
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学科
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农作物 |
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基金
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中国科学院知识创新工程重要方向项目
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文献收藏号
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CSCD:5131417
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