斑马鱼HO1基因的表达特征及功能研究
STUDY ON EXPRESSION PATTERN AND FUNCTION OF ZEBRAFISH HO1
查看参考文献26篇
文摘
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实验对血红素加氧酶1(HO1)在斑马鱼发育中的功能进行了研究。多重序列比对结果显示,斑马鱼HO1与哺乳类、鸟类及其他鱼类的HO1氨基酸序列的总体相似性为44.1%-86.8%,血红素结合标签相似性为87.5%-95.8%。对斑马鱼早期胚胎和成鱼各组织进行RT-PCR检测,结果显示HO1转录本母源存在, HO1 mRNA的表达水平在尾芽期前较低,到咽囊期迅速上升并稳定在较高水平。HO1基因在斑马鱼成鱼多个组织中均有表达,在肝脏、脾、鳃、肾中的表达量较高。WISH结果显示, HO1基因在斑马鱼胚胎的卵黄合胞层、眼和血液中的表达量较高。利用超表达和基因敲降技术发现,注射HO1 mRNA使HO1基因过表达对斑马鱼早期胚胎发育无明显影响。注射HO1 MO使HO1基因表达抑制可导致斑马鱼胚胎出现发育迟缓、围心腔水肿、尾部消失等不同程度的畸形。HO1 MO导致的斑马鱼胚胎发育异常可被HO1 mRNA回复。利用Real-Time PCR研究发现, HO1基因表达抑制可导致IGF1表达量显著下降, IGFBP1表达量显著升高。这些结果表明斑马鱼HO1基因可通过调节IGF信号途径调控胚胎的正常发育。 |
其他语种文摘
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This paper has studied the function of heme oxygenase 1 (HO1) in the development of zebrafish. Zebrafish HO1 contains heme oxygenase domain, heme binding signature, five histidine residues and hydrophobic segment at carboxyl terminus. Analysis of the deduced amino acid sequences revealed zebrafish HO1 shared 44.1%-86.8% similarity with known sequences from other species. Analyzing embryos of different development stages by RT-PCR showed that zebrafish HO1 existed in unfertilized eggs. Its level increased from 24hpf and remained a high level expression until 72hpf. The tissue expression pattern analysis showed that zabrafish HO1 was expressed ubiquitously and the expression in brain, heart, gill and liver was obviously higher than others. Whole-mount in situ hybridization showed that HO1 transcript exists in yolk syncytial layer, blood and eyes during embyonic development. Using overexpression and knock down technology, we found that over-expression of HO1 had no apparent effect on the early development of zebrafish. Knocked down of HO1 gene by HO1 MO made the embryo development retarded. The abnormality and lethal rates of embryos after MO treatment were significantly increased compared with control. The level of IGF1 and IGFBP1 mRNA changed significantly after HO1 was knocked down. These results showed that HO1 can regulate of zebrafish embryonic development through IGF signaling. |
来源
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水生生物学报
,2014,38(2):209-215 【核心库】
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关键词
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斑马鱼
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血红素加氧酶1
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表达特征
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功能
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地址
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1.
华中师范大学生命科学学院, 湖北省遗传调控与整合生物学重点实验室, 武汉, 430079
2.
中国科学院水生生物研究所, 淡水生态与生物技术国家重点实验室, 武汉, 430072
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语种
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中文 |
文献类型
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研究性论文 |
ISSN
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1000-3207 |
学科
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遗传学 |
基金
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国家自然科学基金项目
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中国科学院水生生物研究所淡水生态与生物技术国家重点实验室基金
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文献收藏号
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CSCD:5103839
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参考文献 共
26
共2页
|
1.
Shibahara S. Functional analysis of cDNAs for two types of human heme oxygenase and evidence for their separate regulation.
The Journal of Biochemistry,1993,113(2):214-218
|
被引
5
次
|
|
|
|
2.
McCoubrey W K Jr. Isolation and characterization of a cDNA from the rat brain that encodes hemoprotein heme oxygenase-3.
European Journal of Biochemistry,1997,247(2):725-732
|
被引
19
次
|
|
|
|
3.
孙健乐. 保护基因HO在组织细胞中的作用及其机制研究进展.
生命科学,2003,15(4):220-223
|
被引
3
次
|
|
|
|
4.
Ryter S W. Heme oxygenase-1/carbon monoxide: from basic science to therapeutic applications.
Physiological Reviews,2006,86:583-650
|
被引
78
次
|
|
|
|
5.
Lin Q. Heme Oxygenase-1 protein localizes to the nucleus and activates transcription factors important in oxidative stress.
The Journal of Biological Chemistry,2007,282(28):20621-20633
|
被引
10
次
|
|
|
|
6.
Pachori A S. Heme-oxygenase- 1-induced protection against hypoxia/reoxygenation is dependent on biliverdin reductase and its interaction with PI3K/Akt pathway.
Journal of Molecular and Cellular Cardiology,2007,43(5):580-592
|
被引
11
次
|
|
|
|
7.
Hachfi L. Effect of water temperature increase on HO-1 expression in European sea bass (Dicentrarchus labrax L.) tissues.
Cellular and Molecular Biology,2012,58(Suppl):1752-1756
|
被引
2
次
|
|
|
|
8.
Bainbridge S A. HO in pregnancy.
Free Radical Biology and Medicine,2005,38(8):979-988
|
被引
4
次
|
|
|
|
9.
Zhao H. Effect of heme oxygenase-1 deficiency on placental development.
Placenta,2009,30(10):861-868
|
被引
4
次
|
|
|
|
10.
Yachie A. Oxidative stress causes enhanced endothelial cell injury in human heme oxygenase-1 deficiency.
The Journal of Clinical Investigation,1999,103(1):129-135
|
被引
33
次
|
|
|
|
11.
Kartikasari A E. Hepcidin suppression and defective iron recycling account for dysregulation of iron homeostasis in heme oxygenase-1 deficiency.
Journal of Cellular and Molecular Medicine,2009,13(9B):3091-3102
|
被引
1
次
|
|
|
|
12.
Kovtunovych G. Dysfunction of the heme recycling system in heme oxygenase 1-deficient mice: effects on macrophage viability and tissue iron distribution.
Blood,2010,116(26):6054-6062
|
被引
4
次
|
|
|
|
13.
Kreiser D. Heme oxygenase-1 modulates fetal growth in the rat.
Laboratory Investigation,2002,82(6):687-692
|
被引
2
次
|
|
|
|
14.
Poss K D. Reduced stress defense in heme oxygenase 1-deficientcells.
Proceedings of the National Academy of Sciences USA,1997,94(20):10925-10930
|
被引
17
次
|
|
|
|
15.
Livak K J. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-??Ct) method.
Methods,2001,25(4):402-408
|
被引
8359
次
|
|
|
|
16.
尹隽. 银鲫pou2基因在胚胎发育过程中的时空表达图式.
水生生物学报,2007,31(5):629-636
|
被引
5
次
|
|
|
|
17.
王锐. 银鲫果糖-1,6-二磷酸酶的分子克隆与表达分析.
水生生物学报,2010,34(6):1130-1135
|
被引
7
次
|
|
|
|
18.
Watanabe T. Expression of heme oxygenase-1 in rat ontogeny.
Archives of Histology and Cytology,2003,66(2):155-162
|
被引
2
次
|
|
|
|
19.
Watanabe S. Marked developmental changes in heme oxygenase-1 (HO-1) expression in the mouse placenta: correlation between HO-1 expression and placental development.
Placenta,2004,25(5):387-395
|
被引
1
次
|
|
|
|
20.
Fraser S T. Heme Oxygenase-1:A critical link between ironmetabolism, erythropoiesis, and development.
Advances in Hematology,2011:Article ID 473709,6 pages
|
被引
1
次
|
|
|
|
|