微波辅助酶促月桂酸淀粉酯的合成
Lipase-Catalyzed Esterification of Starch Using Lauric Acid under Microwave
查看参考文献10篇
|
文摘
|
以天然玉米淀粉和月桂酸为原料, 脂肪酶Novozym 435为催化剂, 研究了微波辐照下月桂酸淀粉酯的酶促合成. 通过对淀粉进行预处理活化来提高淀粉的酯化反应活性, 并主要考察了微波功率、 月桂酸用量、 脂肪酶Novozym 435用量及反应时间等参数对酶促月桂酸淀粉酯合成的影响. 采用气相色谱法进行取代度的测定, 并以取代度为考察指标, 确定了最佳的反应条件. 结果表明, 淀粉的最佳预处理方法为NaOH/尿素混合溶液法, 经该法处理后淀粉的酯化反应活性大大提高; 微波辐照技术的应用大大缩短了反应时间, 并提高了月桂酸淀粉酯的取代度. 适宜的工艺条件为: 微波功率为240 W, 月桂酸用量为30%, 酶加入量为7%, 反应时间26 min. |
|
其他语种文摘
|
Lauric acid starch ester was synthesized by using immobilized lipase Novozym 435 as the biocatalyst under microwave. The esterification activity was been improved by pretreatment. Several factors, such as the microwave power, amount of lipase Novozym 435, amount of lauric acid and esterification time has been investigated. A novel GC-based method was used to measure the degree of substitution (DS) of lauric acid starch ester. DS as the research object was adopt to optimize the reaction conditions. According to the results of the single-factor experiments, the perpendicular experiments were designed to optimize the parameters of the reaction. The results showed that the optimum reaction conditions were as below: microwave power was 240 W, the amount of lauric acid was 30%, the amount of lipase was 7% and the reaction time was 26 min. A degree of substitution of 0.0311 was obtained. The structures of products were characterized by FT-IR spectra. |
|
来源
|
分子催化
,2014,28(1):67-74 【核心库】
|
|
关键词
|
淀粉预处理
;
微波辐照
;
脂肪酶
;
月桂酸淀粉酯
|
|
地址
|
1.
哈尔滨商业大学, 黑龙江省省高校食品科学与工程重点实验室, 黑龙江, 哈尔滨, 150076
2.
哈尔滨商业大学, 黑龙江省省高校食品科学与工程重点实验室;;羰基合成与选择氧化国家重点实验室, 黑龙江, 哈尔滨, 150076
3.
中国科学院兰州化学物理研究所, 羰基合成与选择氧化国家重点实验室, 甘肃, 兰州, 730000
|
|
语种
|
中文 |
|
文献类型
|
研究性论文 |
|
ISSN
|
1001-3555 |
|
学科
|
化学 |
|
基金
|
黑龙江省教育厅科技创新团队计划
;
国家自然科学基金
;
黑龙江省杰出青年科学基金
|
|
文献收藏号
|
CSCD:5078512
|
参考文献 共
10
共1页
|
|
1.
Brain W P. The first hundred years corn reifining in the United States.
Starch,2001,53:257-260
|
被引
1
次
|
|
|
|
|
2.
张燕萍.
变性淀粉制造与应用,2001:2
|
被引
1
次
|
|
|
|
|
3.
Dejan B. The effect of substrate polarity on the lipase-catalyzed synthesis of aroma esters in solvent-free systems.
Journal of Molecular Catalysis B: Enzymatic,2007,45(4):97-101
|
被引
2
次
|
|
|
|
|
4.
Takashi K. Lipase-catalyzed condensation of p-methoxy-phenethyl alcohol and carboxylic acids with different steric and electrical properties in acetonitrile.
Biotechnology Letters,2003,25(1):3-7
|
被引
3
次
|
|
|
|
|
5.
Marcin L. Low power microwave-assisted enzymatic esterification of starch.
Starch/Starke,2012,64:188-197
|
被引
1
次
|
|
|
|
|
6.
Jia Y X. Biosysthesis of corn starch palmitate by lipase Novozym 435.
International Journal of Molecular Sciences,2012,13:7227-7234
|
被引
1
次
|
|
|
|
|
7.
Jia Y X. Influence of pretreatment on cold water solubility and esterification activity of starch.
Advance Journal of Food Science and Technology,2012,4(5):270-276
|
被引
1
次
|
|
|
|
|
8.
冀国强. 超声波技术在淀粉改性中应用.
粮食与油脂,2010,1:1-4
|
被引
1
次
|
|
|
|
|
9.
王艳. 无溶剂体系酶催化油酸淀粉酯的合成.
中国粮油学报,2012,27(11):39-49
|
被引
7
次
|
|
|
|
|
10.
Huang M F. Studies on the properties of montmorillonite-reinforced thermoplastic starch composites.
Polymer,2004,45:7017-7023
|
被引
19
次
|
|
|
|
|
了解气象卫星更多信息，请访问