斜带石斑鱼MyD88基因的原核表达及蛋白纯化
PROKARYOTIC EXPRESSION AND PROTEIN PURIFICATION OF THE MYD88 GENE IN GROUPER EPINEPHELUS COIOIDES
查看参考文献15篇
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文摘
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Toll样受体(Toll-like receptor, TLR)是一类重要的模式识别受体, 其胞外结构域可识别特定的病原相关分子模式(Pathogen-associated molecular patterns, PAMPs), 胞内的TIR (Toll-like/IL-1 receptor)结构域则参与启动胞内信号转导, 诱导细胞产生活性氧中间体(Reactive oxygen intermediate)、活性氮中间体(Reactive nitrogen intermediate)和促炎症因子, 在感染早期发挥重要作用。在哺乳动物中, 髓样分化因子88(Myeloid differentiation factor 88, MyD88)是TLR信号通路的关键接头分子, 其C端TIR域与TLR的TIR相互结合后, 通过募集IL-1受体相关激酶(Interleukin-1 receptor-associated kinase, IRAK)IRAK4、IRAK1和IRAK2, 激活NF-κB和MAPK, 进而诱导IL-1、TNF和IFN等细胞因子表达。 |
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来源
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水生生物学报
,2014,38(1):200-202 【核心库】
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关键词
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斜带石斑鱼
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MyD88
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原核表达
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蛋白纯化
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地址
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1.
广西大学动物科学技术学院, 淡水生态与生物技术国家重点实验室, 南宁, 530004
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广西大学动物科学技术学院, 南宁, 530004
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中国科学院水生生物研究所, 淡水生态与生物技术国家重点实验室, 武汉, 430072
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语种
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中文 |
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文献类型
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研究性论文 |
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ISSN
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1000-3207 |
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学科
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分子生物学 |
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基金
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国家973计划
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国家自然科学基金面上项目
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广西自然科学基金
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文献收藏号
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CSCD:5046718
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参考文献 共
15
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