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斑马鱼ca15b的克隆及在原始生殖细胞中的特异表达
CLONING, IDENTIFICATION AND EXPRESSION ANALYSIS OF CA15B, A NOVEL GENE SPECIFICALLY EXPRESSED IN PRIMORDIAL GERM CELLS OF ZEBRAFISH

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文摘 高保真PCR克隆获得了ca15b基因的全长, 利用胚胎整体原位杂交等技术研究了ca15b基因在斑马鱼早期发育过程中的动态表达。结果发现, ca15b在斑马鱼早期发育过程中存在显著的原始生殖细胞(Primordial germ cell, PGC)特异表达模式。ca15b是一个母源性表达的基因: 在分裂期的胚胎中, 其mRNA集中分布于位于分裂沟的生殖质(Germ plasm); 从囊胚期开始, 可以观察到其在PGC中的特异表达; 在原肠胚中, 其mRNA在体细胞中急剧降解, 仅特异表达于PGC, 这一表达特征一直持续到受精后1d的胚胎。将体外合成的包含5′UTR和3′UTR的ca15b全长mRNA注射到斑马鱼胚胎后, 仅能增强原肠期之前胚胎中ca15b的整体杂交信号; 在原肠胚期之后, 注射的mRNA在体细胞中快速降解。这提示在ca15b mRNA上可能存在某种转录后调控其在早期胚胎体细胞中降解而在PGC中稳定存在的机制。
其他语种文摘 Primordial germ cells (PGCs) are the ancestor cells of adult gametes at embryonic stage. PGCs-specific gene expression plays a fundamental role in germ cell induction, development, migration and differentiation. Understanding the functional role of PGCs-specifically expressed genes could provide us some tools to perform gene and embryo manipulations. Screening a public database of zebrafish (http://www.zfin.org) we found a PGCs-specific candidate gene, carbonic anhydrase 15b (ca15b). To confirm this, whole-mount in situ hybridization and other technologies were utilized to analyze the expression of ca15b during zebrafish early development. We observed that ca15b is a maternally expressed gene and it is highly expressed in germ plasm during cleavage stage. The expression of ca15b at mRNA level was specifically detected in the PGCs at blastula stage. At gastrula stage, ca15b is still strictly expressed in PGCs while its expression is undetectable in somatic cells. This specific expression pattern of ca15b persisted until one day post-fertilization. More importantly, the post-transcriptional regulation elements that mediate PGCs-specific expression of ca15b may locate in its coding sequence.
来源 水生生物学报 ,2014,38(1):142-149 【核心库】
关键词 斑马鱼 ; 原始生殖细胞 ; 表达模式 ; ca15b
地址

中国科学院水生生物研究所, 淡水生态与生物技术国家重点实验室, 武汉, 430072

语种 中文
文献类型 研究性论文
ISSN 1000-3207
学科 遗传学
基金 国家973计划 ;  国家自然科学基金优秀青年科学基金
文献收藏号 CSCD:5046708

参考文献 共 40 共2页

1.  Wylie C. Germ cells. Current Opinion in Genetics & Development,2000,10(4):410-413 被引 7    
2.  Xu H. Fish germ cells. Science China Life Sciences,2010,53(4):435-446 被引 4    
3.  Yoon C. Zebrafish vasa homologue RNA is localized to the cleavage planes of 2-and 4-cell-stage embryos and is expressed in the primordial germ cells. Development,1997,124(16):3157-3165 被引 45    
4.  Knaut H. Zebrafish vasa RNA but not its protein is a component of the germ plasm and segregates asymmetrically before germline specification. Journal of Cell Biology,2000,149(4):875-888 被引 16    
5.  Hashimoto Y. Localized maternal factors are required for zebrafish germ cell formation. Developmental Biology,2004,268(1):152-161 被引 7    
6.  Gavis E R. A conserved 90 nucleotide element mediates translational repression of nanos RNA. Development,1996,122(9):2791-2800 被引 7    
7.  Styhler S. vasa is required for GURKEN accumulation in the oocyte, and is involved in oocyte differentiation and germline cyst development. Development,1998,125(9):1569-1578 被引 12    
8.  Shinomiya A. The vasa-like gene, olvas, identifies the migration path of primordial germ cells during embryonic body formation stage in the medaka, Oryzias latipes. Development, Growth & Differentiation,2000,42(4):317-326 被引 16    
9.  Yoshizaki G. Cloning and characterization of a vasa-like gene in rainbow trout and its expression in the germ cell lineage. Molecular Reproduction and Development,2000,55(4):364-371 被引 10    
10.  Koprunner M. A zebrafish nanos-related gene is essential for the development of primordial germ cells. Genes & Development,2001,15(21):2877-2885 被引 22    
11.  Weidinger G. Dead end, a Novel Vertebrate Germ Plasm Component, Is Required for Zebrafish Primordial Germ Cell Migration and Survival. Current Biology,2003,13(16):1429-1434 被引 22    
12.  Kloc M. Two distinct pathways for the localization of RNAs at the vegetal cortex in Xenopus oocytes. Development,1995,121(2):287-297 被引 2    
13.  King M L. Putting RNAs in the right place at the right time: RNA localization in the frog oocyte. Biology of the Cell,2005,97(1):19-33 被引 3    
14.  Mafra D. Erythrocyte zinc and carbonic anhydrase levels in nondialyzed chronic kidney disease patients. Clinical Biochemistry,2004,37(1):67-71 被引 5    
15.  Kuo W H. Differential expression of carbonic anhydrase isoenzymes in various types of anemia. Clinica Chimica Acta,2005,351(1/2):79-86 被引 8    
16.  Westerfield M. A Guide for the Laboratory Use of Zebrafish (Danio rerio) (4th ed),2000:253-309 被引 1    
17.  Lu G. Vector NTI, a balanced all-in-one sequence analysis suite. Briefings in Bioinformatics,2004,5(4):378-388 被引 22    
18.  Letunic I. SMART 4.0: towards genomic data integration. Nucleic Acids Research,2004,32(Database issue):D142-D144 被引 29    
19.  Thompson J D. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research,1994,22(22):4673-4680 被引 1046    
20.  Tamura K. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular Biology and Evolution,2011,28(10):2731-2739 被引 2112    
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