芽孢杆菌SeRB-2还原亚硒酸盐的动力学研究
Study on Kinetics of Selenite Reduction by Bacillus sp.SeRB-2
查看参考文献25篇
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文摘
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微生物还原亚硒酸盐的动力学研究具有重要的现实意义,可以为Se(IV)污染场地的微生物修复设计提供理论依据。本文研究了兼性厌氧菌Bacillus sp.SeRB-2对亚硒酸钠的还原动力学。通过指数方程模型、对数方程模型和米氏方程模型的分析可知,Se(IV)的细菌还原符合一级反应动力学,还原反应主要集中在对数期和稳定生长前期,米氏方程模型能更好的反映细菌对亚硒酸盐的还原过程。通过对不同Se(IV)浓度下的米氏常数(K_m)和最大反应速率(V_(max))的分析发现,当Se(IV)浓度较低时,K_m值较小,V_(max)值较大,这表明Se(IV)浓度越低,还原亚硒酸盐的酶与Se(IV)的结合能力越强,此时细菌对亚硒酸盐的还原速率越大、还原效率也越高。在本研究中,当Se(IV)浓度为1mmol/L时,其还原效率最高可达90%,能够有效去除或降低Se(IV)污染,说明该菌在Se(IV)污染场地的生物修复上具有应用潜力。 |
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其他语种文摘
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It is significant to study the bioreduction kinetics of selenite,which may provide a theoretical basis for bioremediation design in the contaminated sites.The kinetics of Se(IV)reduction by facultative anaerobic strain Bacillus sp.SeRB-2was studied using the index equation model,logarithm equation model and Michaelis-Menten equation model.It is shown that Se(IV)bioreduction fits a first-order reaction,and reacts mainly in the logarithmic phase and early stationary phase.The Michaelis-Menten equation model better reflects the reduction process compared with both index and logarithm equation models.The kinetic parameters K_m and V_(max) acquired were relevant to Se(IV)concentrations,i.e.,the lower the Se(IV)concentrations, the smaller the K_m value and the hither V_(max) will be.It is indicated that the binding ability between Se-(IV)-reducing enzyme and selenite is stronger,the reducing rate is larger and the reducing efficiency is higher,in a lower Se(IV)medium.In addition, the reduction efficiency is up to 90%,when Se(IV)concentrations are about 1mmol/L,which illustrates that Se(IV) contamination can be effectively reduced or removed by strain SeRB-2.This strain may be suitable for bioremediation in the Se(IV)contaminated sites. |
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来源
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地球与环境
,2014,42(1):47-54 【核心库】
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关键词
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细菌
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还原
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亚硒酸盐
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动力学
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参数
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地址
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中国科学院地球化学研究所, 贵阳, 550002
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语种
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中文 |
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文献类型
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研究性论文 |
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ISSN
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1672-9250 |
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学科
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化学 |
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基金
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国家自然科学基金创新研究群体项目
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中国科学院知识创新工程重要方向项目
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国家自然科学基金项目
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文献收藏号
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CSCD:5040945
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25
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