RNA中6-甲基腺嘌呤的研究进展
Recent progresses in RNA N~6-methyladenosine research
查看参考文献56篇
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文摘
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RNA酶促共价修饰研究, 尤其是m~6A(6-甲基腺嘌呤), 是RNA生物学研究的一个新兴领域。m~6A是真核生物mRNA内部序列中最常见的一种转录后修饰形式, 由包含3个独立组分的复合物mRNA: m~6A甲基转移酶催化生成。最新研究发现肥胖相关蛋白FTO可以脱掉m~6A上的甲基, 表明该甲基化过程是可逆的。抑制或敲除m~6A甲基转移酶会引起重要的表型变化, 但是由于过去的检测方法受限, m~6A确切的作用机制目前为止还不甚清楚。二代测序技术结合免疫沉淀方法为大规模检测m~6A修饰并研究其作用机制提供了可能。文章主要综述了m~6A的发现史、生成机制、组织和基因组分布、检测方法、生物学功能等及其最新研究进展, 并通过比较3种IP-seq技术和数据分析的异同及优缺点, 对m~6A这种RNA表观修饰研究中尚未解决的问题进行了讨论。 |
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其他语种文摘
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RNA modifications, especially methylation of the N~6 position of adenosine (A)––m~6A, represent an emerging research territory in RNA biology. m~6A is a post-transcriptional modification of RNAs, which is catalyzed by the mRNA: m~6A methyltransferase complex containing three individual components and is the most common form found in the internal sequences of mRNAs in eukaryotes. Latest study showed that the fat mass and obesity-associated protein could remove the methyl group, indicating that the modification is reversible. Importantly, inhibiting or silencing the methyltransferase will cause significant changes of phenotypes. However, due to limited detection methods, the mechanism of m~6A has not been figured out yet. Next-generation sequencing combining with IP (immunoprecipitation) technologies makes it possible to detect m~6A modifications in a large scale. Here, we reviewed recent progresses of m~6A studies including the discovery of m~6A, mechanism of biosynthesis, tissue and genome distribution, detection methodology and possible biological functions. We also compared three IP-seq technologies that are currently widely used, and summarized the challenges in m~6A studies. |
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来源
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遗传
,2013,35(12):1340-1351 【核心库】
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关键词
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RNA修饰
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6-甲基腺嘌呤
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IP-seq
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地址
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1.
中国科学院北京基因组研究所, 中国科学院基因组学与信息重点实验室, 北京, 100101
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中国科学院北京基因组研究所所级中心, 北京, 100101
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语种
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中文 |
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文献类型
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综述型 |
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ISSN
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0253-9772 |
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学科
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医药、卫生 |
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基金
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国家自然科学基金项目
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北京市科技新星计划项目
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文献收藏号
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CSCD:5017987
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参考文献 共
56
共3页
|
|
1.
Sharp P A. The Centrality of RNA.
Cell,2009,136(4):577-580
|
被引
15
次
|
|
|
|
|
2.
Cantara W A. The RNA modification database, RNAMDB: 2011 update.
Nucleic Acids Res,2010,39(Database):D195-D201
|
被引
29
次
|
|
|
|
|
3.
Dunn D B. Occurrence of a new base in the deoxyribonucleic acid of a strain of Bacterium Coli.
Nature,1955,175(4451):336-337
|
被引
12
次
|
|
|
|
|
4.
Dunn D B. The occurrence of 6-methylaminopurine in deoxyribonucleic acids.
Biochem J,1958,68(4):627-636
|
被引
3
次
|
|
|
|
|
5.
Littlefield J W. Natural occurrence of thymine and three methylated adenine bases in several ribonucleic acids.
Nature,1958,181(4604):254-255
|
被引
2
次
|
|
|
|
|
6.
Jelinek W. Further evidence on the nuclear origin and transfer to the cytoplasm of polyadenylic acid sequences in mammalian cell RNA.
J Mol Biol,1973,75(3):515-532
|
被引
1
次
|
|
|
|
|
7.
Desrosie R. Identification of methylated nucleosides in messenger RNA from Novikoff hepatoma cells.
Proc Natl Acad Sci USA,1974,71(10):3971-3975
|
被引
117
次
|
|
|
|
|
8.
Adams J M. Modified nucleosides and bizarre 5′-termini in mouse myeloma mRNA.
Nature,1975,255(5503):28-33
|
被引
20
次
|
|
|
|
|
9.
Desrosiers R C. Characterization of Novikoff hepatoma mRNA methylation and heterogeneity in the methylated 5' terminus.
Biochemistry,1975,14(20):4367-4374
|
被引
8
次
|
|
|
|
|
10.
Dubin D T. The methylation state of poly A-containing-messenger RNA from cultured hamster cells.
Nucleic Acids Res,1975,2(10):1653-1668
|
被引
24
次
|
|
|
|
|
11.
Furuichi Y. Reovirus messenger RNA contains a methylated, blocked 5′-terminal structure: m-7G(5′)ppp(5′)G-MpCp-.
Proc Natl Acad Sci USA,1975,72(1):362-366
|
被引
1
次
|
|
|
|
|
12.
Perry R P. The methylated constituents of L cell messenger RNA: evidence for an unusual cluster at the 5′ terminus.
Cell,1975,4(4):387-394
|
被引
11
次
|
|
|
|
|
13.
Haugland R A. Post-transcriptional modifications of oat coleoptile ribonucleic acids. 5′-Terminal capping and methylation of internal nucleosides in poly(A)-rich RNA.
Eur J Biochem,1980,104(1):271-277
|
被引
7
次
|
|
|
|
|
14.
Beemon K. Localization of N6-methyladenosine in the Rous sarcoma virus genome.
J Mol Biol,1977,113(1):165-179
|
被引
12
次
|
|
|
|
|
15.
Moss B. 5′-Terminal and internal methylated nucleosides in herpes simplex virus type 1 mRNA.
J Virol,1977,23(2):234-239
|
被引
4
次
|
|
|
|
|
16.
Chen-Kiang S. N-6-methyl-adenosine in adenovirus type 2 nuclear RNA is conserved in the formation of messenger RNA.
J Mol Biol,1979,135(3):733-752
|
被引
8
次
|
|
|
|
|
17.
Narayan P. An in vitro system for accurate methylation of internal adenosine residues in messenger RNA.
Science,1988,242(4882):1159-1162
|
被引
11
次
|
|
|
|
|
18.
Tuck M T. Partial purification of a 6-methyladenine mRNA methyltransferase which modifies internal adenine residues.
Biochem J,1992,288(Pt 1):233-240
|
被引
11
次
|
|
|
|
|
19.
Bokar J A. Characterization and partial purification of mRNA N6-adenosine methyltransferase from HeLa cell nuclei. Internal mRNA methylation requires a multisubunit complex.
J Biol Chem,1994,269(26):17697-17704
|
被引
43
次
|
|
|
|
|
20.
Bokar J A. Purification and cDNA cloning of the AdoMet-binding subunit of the human mRNA (N6-adenosine)-methyltransferase.
RNA,1997,3(11):1233-1247
|
被引
80
次
|
|
|
|
|
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