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Evaluation of three tracers for labeling distal cerebrospinal fluidcontacting neurons

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文摘 It has been reported that distal cerebrospinal fluid-contacting neurons (dCSF-CNs) can be detected by immunohistochemical assay using cholera toxin subunit B-conjugated horseradish peroxidase (CBHRP). In the present study, another two methods with CB alone or CB-conjugated FITC (CB-FITC) were used, and the results from the three methods were compared. Adult Sprague-Dawley rats were randomly divided into three groups with CB-HRP, CB or CB-FITC. Tracers were diluted to 30% in artificial cerebrospinal fluid and injected separately (in a volume of 3 μL) into the lateral ventricle. Animals from the CB-HRP and CB groups were perfused 48 h after surgery while animals from the CB-FITC group were perfused 1, 3, 6, 12, 24 or 48 h after surgery. The brain was sectioned (40 μm) for immunofluorescence and five sections with positive neurons were selected from each rat for neuron counting. Three clusters of positive neurons in a 'Y-like' distribution were detected ventral to the cerebral aqueduct of rats from the three groups. No significant difference was observed among the quantitative data. In the CB-FITC group, stable staining was detected even at 6 h after injection. Taken together, lateral ventricle injection of CB/CB-FITC is a useful method for labeling dCSFCNs in rats. The CB-FITC method makes dCSF-CNs labeling much simpler and more convenient.
来源 Neuroscience Bulletin ,2013,29(5):576-580 【核心库】
DOI 10.1007/s12264-013-1332-0
关键词 distal cerebrospinal fluid-contacting neurons ; cholera toxin subunit B-conjugated horseradish peroxidase ; cholera toxin subunit B ; cholera toxin subunit B-conjugated FITC ; label
地址

1. Department of Anesthesiology, The First Clinical College, China Medical University, Jiangsu Province Key Laboratory of Anesthesiology;;Jiangsu Key Laboratory of Brain Disease Bioinformation, Shenyang, 110001  

2. Xuzhou Medical College, Jiangsu Province Key Laboratory of Anesthesiology, Xuzhou, 221002  

3. Department of Anesthesiology, The First Clinical College, China Medical University, Jiangsu Province Key Laboratory of Anesthesiology, Shenyang, 110001

语种 英文
文献类型 研究性论文
ISSN 1673-7067
学科 外科学
基金 the Natural Science Foundation of the Jiangsu Higher Education Institutions of China ;  Open Project Funds of the Jiangsu Key Laboratory of Brain Disease Bioinformation ;  the Fund of Science and Technology Innovation Team in Jiangsu Province, China (2009) ;  国家自然科学基金
文献收藏号 CSCD:4975289

参考文献 共 14 共1页

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