The cytoplasmic droplet may be indicative of sperm motility and normal spermiogenesis
查看参考文献33篇
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文摘
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Although the cytoplasm of spermatids is removed at the end of spermiogenesis, a tiny portion is usually retained in the sperm flagellum, which is termed the cytoplasmic droplet (CD) in mammals. CDs are believed to play a role in sperm volume adaptation. However, we have noticed that epididymat spermatozoa that display initial (flagellation in situ) and progressive motility mostly possess CDs, whereas spermatozoa without CDs are rarely motile, suggesting that CDs have a role in motility development during sperm epididymat maturation. In the present study, we analyzed the relationship between the presence or absence of CDs, motility development and positional changes of CDs during sperm epididymal maturation in mice and monkeys. We also examined CDs on spermatozoa of three knockout mouse lines with late spermiogenic defects. Our data suggest that the CD is a normal organelle transiently present exclusively on epididymal spermatozoa, and normal CD morphology and location are associated with normal motility development during epididymal maturation of spermatozoa. Abnormal CD formation, e.g., a complete lack of CDs or ectopic CDs, is indicative of defective spermiogenesis. If CDs are essential for sperm motility development, then CDs may represent an ideal drug target for the development of non-hormonal male contraceptives. |
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来源
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Asian Journal of Andrology
,2013,15(6):799-805 【核心库】
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DOI
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10.1038/aja.2013.69
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关键词
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cytoplasmic droplets
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epididymis
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fertility
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mouse
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semen analysis
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spermiogenesis
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sperm motility
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地址
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Department of Laboratory Animal Medicine, China Medical University, Shenyang, 110001
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语种
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英文 |
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文献类型
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研究性论文 |
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ISSN
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1008-682X |
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学科
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外科学 |
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基金
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supported in part by startup funds from the University of Nevada, Reno (to WY).
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文献收藏号
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CSCD:4960262
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参考文献 共
33
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