海岛棉GbMFT1基因的克隆及表达分析
Cloning and Expression Analysis of GbMFT1 Gene in Gossypium barbadense L
查看参考文献35篇
|
文摘
|
通过RT-PCR和RACE技术,从新疆海岛棉品种新海14中克隆得到了一个MFT(MOTHER OF FT AND TFL1)类似基因,命名为GbMFT1基因(GenBank登录号为KC513744)。GbMFT1基因的开放阅读框(ORF)为528 bp,编码175个氨基酸的蛋白,含一个磷脂酰乙醇胺结合蛋白(PEBP)结构域。GbMFT1蛋白的羧基端含有MFT蛋白都含有的脯氨酸。系统进化树分析表明GbMFT1编码产物与葡萄、番茄亲缘关系较近,属于同一进化分支。实时荧光定量PCR分析表明,GbMFT1基因在棉花的不同组织中均有表达,在花瓣中的表达量较高;在纤维发育的不同时期中均有表达,在开花后2 d的胚珠、9 d的纤维中表达量最高。半定量RT-PCR结果表明,GbMFT1基因在刚萌发的种子中表达量高,用不同浓度的ABA处理种子后其表达变化不明显,表明GbMFT1基因的表达不受ABA的调节。 |
|
其他语种文摘
|
Angiosperm genes sharing a conserved phosphatidylethanolamine-binding (PEBP) domain have been shown to be involved in the control of flowering time.The family is divided into three subfamilies, FT-like, TFL1-like and MFT-like. Evolution analysis showed that MOTHER OF FT AND TFL1 (MFT) is the ancestral form of all PEBP genes in plant; however the exact fuction of MFT is still unknown. One MFT-like homolog, designated GbMFT1, was isolated from Gossypium barbadense L. cv. Xinhai 14 using RT-PCR and RACE methods. The open reading frame of GbMFT1 is 528 bp in length, which encodes a putative protein of 175 amino acids. GbMFT1 gene has a PEBP conserved motif. In consistency with almost all MFT-like proteins, GbMFT1 has a conserved proline residue near the C-terminus. Phylogenetic analysis revealed that GbMFT1 has high sequence similarities with VvMFT and SP2G and they belong to the same clade. The result of qRT-PCR assay indicated that GbMFT1 expressed in different tissues of cotton, with a maximum expression in petal, and in different developmental stages of fibre, especially in two-day ovule and nine-day fibre. Semiquantitative RT-PCR assay indicated that GbMFT1 had a high level of transcription at the beginning of seed germination. Exogenous abscisic acid (ABA) of different concentrations resulted in no significant change in GbMFT1 expression in germinating seeds, indicating that the expression of this gene is not regulated by ABA. |
|
来源
|
作物学报
,2013,39(8):1391-1399 【核心库】
|
|
关键词
|
GbMFT1基因
;
早花
;
表达分析
;
成花素
|
|
地址
|
1.
石河子大学生命科学学院, 农业生物技术国家重点实验室, 新疆, 石河子, 832003
2.
中国科学院新疆理化技术研究所, 中国科学院干旱区植物资源化学重点实验室, 新疆, 乌鲁木齐, 830011
|
|
语种
|
中文 |
|
文献类型
|
研究性论文 |
|
ISSN
|
0496-3490 |
|
学科
|
农作物 |
|
基金
|
国家转基因生物新品种培育重大专项
;
新疆生产建设兵团博士基金
|
|
文献收藏号
|
CSCD:4897542
|
参考文献 共
35
共2页
|
|
1.
Bradley D. Control of inflorescence architecture in Antirrhinum.
Nature,1996,379:791-797
|
被引
35
次
|
|
|
|
|
2.
Bradley D. Inflorescence commitment and architecture in Arabidopsis.
Science,1997,275:80-83
|
被引
69
次
|
|
|
|
|
3.
Kardailsky I. Activation tagging of the floral inducer FT.
Science,1999,286:1962-1965
|
被引
127
次
|
|
|
|
|
4.
Kobayashi Y. A pair of related genes with antagonistic roles in mediating flowering signals.
Science,1999,286:1960-1962
|
被引
111
次
|
|
|
|
|
5.
Hanzawa Y. A single amino acid converts a repressor to an activator of flowering.
Proc Natl Acad Sci USA,2005,102:7748-7753
|
被引
53
次
|
|
|
|
|
6.
Ahn J H. A divergent external loop confers antagonistic activity on floral regulators FT and TFL1.
EMBO J,2006,25:605-614
|
被引
61
次
|
|
|
|
|
7.
Ohshima S. Cloning and molecular analysis of the Arabidopsis gene Terminal Flower 1.
Mol Gen Genet,1997,254:186-194
|
被引
15
次
|
|
|
|
|
8.
Mimida N. Functional divergence of the TFL1-like gene family in Arabidopsis revealed by characterization of a novel homologue.
Genes Cells,2001,6:327-336
|
被引
18
次
|
|
|
|
|
9.
Yoo S Y. Acceleration of flowering by overexpression of MFT (MOTHER OF FT AND TFL1).
Mol Cells,2004,17:95-101
|
被引
18
次
|
|
|
|
|
10.
Yamaguchi A. TWIN SISTER OF FT (TSF) acts as a floral pathway integrator redundantly with FT.
Plant Cell Physiol,2005,46:1175-1189
|
被引
46
次
|
|
|
|
|
11.
Corbesier L. FT protein movement contributes to long-distance signaling in floral induction of Arabidopsis.
Science,2007,316:1030-1033
|
被引
220
次
|
|
|
|
|
12.
Jaeger K E. FT protein acts as a long-range signal in Arabidopsis.
Curr Biol,2007,17:1050-1054
|
被引
53
次
|
|
|
|
|
13.
Zeevaart J A D. Leaf-produced floral signals.
Curr Opin Plant Biol,2008,11:541-547
|
被引
14
次
|
|
|
|
|
14.
Tamaki S. Hd3a protein is a mobile flowering signal in rice.
Science,2007,316:1033-1036
|
被引
137
次
|
|
|
|
|
15.
Taoka K. 14-3-3 proteins act as intracellular receptors for rice Hd3a florigen.
Nature,2011,476:332-335
|
被引
64
次
|
|
|
|
|
16.
Navarro C. Control of flowering and storage organ formation in potato by FLOWERING LOCUS T.
Nature,2011,478:119-121
|
被引
53
次
|
|
|
|
|
17.
Ruonala R. CENL1 expression in the rib meristem affects stem elongation and the transition to dormancy in Populus.
Plant Cell,2008,20:59-74
|
被引
10
次
|
|
|
|
|
18.
Bohlenius H. CO/FT regulatory module controls timing of flowering and seasonal growth cessation in trees.
Science,2006,312:1040-1043
|
被引
89
次
|
|
|
|
|
19.
Lifschitz E. The tomato FT ortholog triggers systemic signals that regulate growth and flowering and substitute for diverse environmental stimuli.
Proc Natl Acad Sci USA,2006,103:6398-6403
|
被引
48
次
|
|
|
|
|
20.
Hedman H. Early evolution of the MFT-like gene family in plants.
Plant Mol Biol,2009,70:359-369
|
被引
13
次
|
|
|
|
|
了解气象卫星更多信息，请访问