斜带石斑鱼(Epinephelus coioides)GnRH Ⅱ基因的克隆及启动子分析
Cloning and promoter analysis of gonadotrophin-releasing hormone Ⅱ in the orange-spotted grouper (Epinephelus coioides)
查看参考文献27篇
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文摘
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促性腺激素释放激素(Gonadotrophin-releasing hormone,GnRH)是调控脊椎动物生殖的关键因子之一。文章克隆了经济鱼类斜带石斑鱼(Epinephelus coioides)GnRHⅡ基因的cDNA、基因组序列及其启动子,发现GnRHⅡ基因启动子中与组织特异性表达相关的区域位于距转录起始点上游2005 bp～956 bp之间。以GnRHⅡ启动子驱动绿色荧光蛋白在斑马鱼胚胎中表达的结果显示,GnRHⅡ神经元主要分布在中脑附近和眼部。文章为深入了解GnRHⅡ基因表达的调控机制及GnRHⅡ基因的功能提供了基础信息。 |
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其他语种文摘
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Gonadotrophin-releasing hormone (GnRH) is a key regulator of reproduction in all vertebrates. We first cloned the cDNA and genomic DNA sequences coding for GnRHⅡ gene in the orange-spotted grouper (Epinephelis coioides), an economically important marine fish, and then cloned its promoter sequence. The region responsible for the cell-specific expression of GnRHⅡ was located between -2005 bp to -956 bp from the translation start site. GnRHⅡ promoter driven EGFP expression in transgenic zebrafish showed that GnRHⅡ-positive neurons were primarily located in the midbrain and in the eyes. Our results provide an improved understanding of the regulatory mechanism and function of GnRHⅡ of E. coioides. |
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来源
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遗传
,2013,35(7):896-902 【核心库】
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关键词
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斜带石斑鱼
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促性腺激素释放激素(GnRH)
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启动子
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地址
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中国科学院水生生物研究所, 淡水生态与生物技术国家重点实验室, 武汉, 430072
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语种
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中文 |
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文献类型
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研究性论文 |
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ISSN
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0253-9772 |
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学科
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分子生物学 |
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基金
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国家自然科学基金项目
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中国科学院知识创新工程重要方向项目
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国家973计划
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文献收藏号
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CSCD:4882673
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