宽口光唇鱼微卫星位点的筛选与特征分析
Isolation and Characterization Analysis of Microsatellite Loci in Acrossocheilus monticola
查看参考文献25篇
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文摘
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通过FIASCO法(Fast Isolation by AFLP Sequences Containing Repeats)筛选宽口光唇鱼Acrossocheilus monticola(Gunter)基因组微卫星位点,利用生物素标记的寡核苷酸探针(AC)_8、(CT)_8、(GGT)_8、(GATA)_8和(GATT)_7首次成功构建宽口光唇鱼基因组微卫星富集文库。从文库中共筛选495个克隆测序,成功设计163对微卫星引物,经PCR扩增检测,获得了18个多态性微卫星标记。利用这18对多态性引物,分析了赤水河赤水市宽口光唇鱼种群的遗传多样性,数据显示:18对多态性微卫星引物的等位基因数为8~31个,平均等位基因数为19.6个,平均期望杂合度为0.8701,平均观测杂合度为0.8312,其中引物Am 07、Am 35、Am 47、Am 69、Am 78和Am 127显著偏离哈迪温伯格平衡(P<0.05),以上数据表明赤水河赤水市宽口光唇鱼种群的遗传多样性水平较高。筛选的微卫星标记对于宽口光唇鱼的遗传背景分析和生物种质资源的保护有重要作用。 |
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其他语种文摘
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Acrossocheilus monticola is the endemic fish in the upper reach of the Yangtze River. For a long time, little attention has been paid to this population because of its small size and low economic value. In recent years, the construction of hydroelectric projects and some anthropic factors changed the physical environment and hydrological conditions in the Yangtze River, which lead to some impacts on the rare and endemic fishes in the Yangtze River including A. monticola. In this study, we isolated microsatellite loci from A. monticola genome by FIASCO (Fast Isolation by AFLP SequencesContaining Repeats) procedure. Taking advantages of biotinylated oligo probes (AC)_8, (CT)_8, (GGT)_8, (GATA)_8 and (GATT)_7, we successfully constructed a microsatellite-enriched genomic library, and 495 clones were sequenced. 163 pairs of SSR-primers were designed afterwards, and from which 18 polymorphic microsatellite markers were screened out by PCR amplification. Using these polymorphic microsatellite markers, we analyzed the genetic diversity of the population of A. monticola collected from the Chishui city. The results showed that the average allele number of microsatellite loci was 19.6, the average observed heterozygosity (Ho) was 0.8312, and the average expected heterzygosity (He) was 0.8701. Six markers, Am07, Am35, Am47, Am69, Am78 and Am127, were deviated from Hardy-Weinberg equilibrium significantly (P<0.05). These results indicated that the genetic diversity of A. monticola was diversiform, and these polymorphic microsatellite primers could be used in A. monticola population genetic analysis as well as biological resource conservation. |
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来源
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四川动物
,2013,32(2):161-166 【核心库】
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关键词
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宽口光唇鱼
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微卫星
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特征分析
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遗传多样性
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地址
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1.
中国科学院水生生物研究所, 中国科学院水生生物多样性与保护重点实验室, 武汉, 430072
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大连海洋大学, 辽宁, 大连, 116023
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语种
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中文 |
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文献类型
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研究性论文 |
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ISSN
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1000-7083 |
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学科
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分子生物学;动物学 |
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基金
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中国长江三峡集团公司"岩原鲤等二十一种长江上游特有鱼类生物学、种群动态及遗传多样性研究"项目
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水利部公益专项"金沙江下游水电开发中特有鱼类保护关键技术"项目
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文献收藏号
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CSCD:4802207
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参考文献 共
25
共2页
|
|
1.
段友健. 中华金沙鳅多态性微卫星位点的筛选与特征分析.
水生生物学报,2012,36(1):148-151
|
被引
5
次
|
|
|
|
|
2.
葛家春. 利用扩增片断长度多态性技术分析长江刀鲚的遗传多样性.
南京大学学报（自然科学版）,2008,44(3):332-338
|
被引
7
次
|
|
|
|
|
3.
匡刚桥. FIASCO法筛选鳜鱼微卫星标记.
中国水产科学,2007,14(4):608-614
|
被引
15
次
|
|
|
|
|
4.
刘金亮. 东北雅罗鱼微卫星分子标记的筛选及特征分析.
华北农学报,2011,26(1):87-93
|
被引
8
次
|
|
|
|
|
5.
罗芬. 氯化钠浓度对宽口光唇鱼精子活力的影响.
四川动物,1999,18(2):70-72
|
被引
7
次
|
|
|
|
|
6.
鲁双庆. 微卫星标记在鱼类遗传育种研究中的应用.
水利渔业,2003,23(6):9-11
|
被引
4
次
|
|
|
|
|
7.
梅志刚. 长江江豚种群衰退机理研究进展.
生命科学,2011,23(5):519-524
|
被引
8
次
|
|
|
|
|
8.
王奕华. 青岛文昌鱼遗传多样性的RAPD分析.
海洋水产研究,2004,25(3):21-27
|
被引
8
次
|
|
|
|
|
9.
严太明. 宽口光唇鱼胚胎发育的研究.
水生生物学报,1999,23(6):636-640
|
被引
11
次
|
|
|
|
|
10.
张四明. 中华鲟随机扩增多态性DNA及遗传多样性研究.
海洋与湖沼,2000,31(1):1-7
|
被引
31
次
|
|
|
|
|
11.
Balloux. The estimation of population differentiation with microsatellite markers.
Mol Ecol,2002,11(2):155-165
|
被引
94
次
|
|
|
|
|
12.
Bostein D. Construction of a genetic linkage map in man using restriction fragment length polymorphisms.
American J Human Genetics,1980,32(3):314-331
|
被引
108
次
|
|
|
|
|
13.
Brenner S. Characterization of the pufferfish (Fugu) genome as a compact model vertebrate genome.
Nature,1993,366:265-268
|
被引
37
次
|
|
|
|
|
14.
Castano-Scnchez C. A set of polymorphic trinucleotide and tetranucleotide microsatellite markers for the Japanese flounder (Paralichthys olivaceus).
Anim Gen,2007,38:75-76
|
被引
5
次
|
|
|
|
|
15.
Crooijmans R P M A. Microsatellite markers in common carp.
Anim Gen,1997,28:129-132
|
被引
76
次
|
|
|
|
|
16.
Dewoody J A. Microsatellite variation in marine, freshwater and anadromous fishes compared with other animals.
J Fish Biol,2000,56:461-473
|
被引
70
次
|
|
|
|
|
17.
Lindqvist A K B. Chromosome-specific panels of tri-and tetranucleotide microsatellite markers for multiplex fluorescent detection and automated genotyping: evaluation of their utility in pathology and forensics.
Genome Res,1996,6:1170-1176
|
被引
8
次
|
|
|
|
|
18.
Maguire T L. Characterization and analysis of microsatellite loci in mangrove species, Avicenia marina (Fork) Vierh. (Avicenniaceae).
Theor Appl Genet,2000,101:279-285
|
被引
11
次
|
|
|
|
|
19.
Reddy O U K. New dinucleotide and trinucleotide microsatellite marker resourses for cotton genome research.
J Cott Sci,2001,5:103-113
|
被引
28
次
|
|
|
|
|
20.
Serapion J. Bioinformatic mining of type I microsatellites from expressed sequence tags of channel catfish (Ictalurus punctatus).
Mar Biotechnol,2004,6:364-377
|
被引
37
次
|
|
|
|
|
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