美洲黑石斑精子超低温保存研究
An efficient methodology of sperm cryopreservation in Centropristis striata
查看参考文献24篇
|
文摘
|
作者采用2mL的冷存管和程序降温仪高效超低温保存美洲黑石斑(Centropristis striata L.)精子。分析比较了4种不同浓度抗冻剂15%DMSO(二甲基亚砜)、15%EG(乙二醇)、15%PG(丙二醇)和10% Meth(甲醇),5种降温速率(10,15,20,25,30℃/min)和5种解冻温度(30,35,40,45,50℃)对美洲黑石斑精子超低温保存效果的影响。实验结果表明,采用Hanks’作为稀释液,15%DMSO、15% EG、15% PG 作为抗冻剂,30℃/min的降温速率对精液进行超低温保存35℃水浴解冻,冻精激活后获得理想的运动率(>60%)。通过对抗冻剂、降温速率、解冻温度的筛选,作者建立了美洲黑石斑精液高效超低温保存的方法,并对冻精进行长期保存。美洲黑石斑精子超低温保存方法的建立对于海水鱼类精子库的建立以及种质资源的保存和生物多样性的保护具有重要意义。 |
|
其他语种文摘
|
In the present study, Centropristis striata sperm was efficiently cryopreserved using a programmable freezer. The motility of both fresh and post-thaw sperm was investigated in order to optimize the sperm cryopreservation protocols for C. striata. Four cryoprotectants (15% dimethyl sulfoxide, 15% ethylene glycol, 15% propylene glycol and 10% methanol), five cooling rates (10, 15, 20, 25 and 30℃/min) as well as five thawing temperatures (30, 35, 40, 45 and 50 ℃) were designed and tested in the sperm cryopreservation, and their effects on post-thaw sperm motility were studied. Optimal post-thaw motility (>60%) were achieved when using Hanks’s supplemented with 15% DMSO, 15% PG and 15% EG with 30℃/min cooling rate, and 35℃ water bath. An efficient cryopreservation method for C. striata sperm was established by analyzing the effect of cryoprotectants, cooling rates and thaw temperatures. This method is advantageous not only to establish laboratory experiment but also to preserve genetic resources for routine aquaculture hatchery operation. |
|
来源
|
海洋科学
,2013,37(1):76-80 【核心库】
|
|
关键词
|
美洲黑石斑(Centropristis striata L.)
;
精子低温保存
;
抗冻剂
;
精子活力
|
|
地址
|
1.
山东省海水养殖研究所, 山东, 青岛, 266002
2.
中国科学院海洋研究所, 山东, 青岛, 266071
|
|
语种
|
中文 |
|
文献类型
|
研究性论文 |
|
ISSN
|
1000-3096 |
|
学科
|
遗传学 |
|
基金
|
国家948计划
;
中国科学院海洋研究所知识创新工程领域前沿项目
;
国家自然科学基金资助项目
|
|
文献收藏号
|
CSCD:4801854
|
参考文献 共
24
共2页
|
|
1.
Gwo J C. Cryopreservation of sperm from the endangered formosan landlocked salmon (Oncorhynchus masou formosanus).
Theriogenology,1999,51(3):569-582
|
被引
13
次
|
|
|
|
|
2.
Van Der Walt L D. The suitability of using cryopreservation of spermatozoa for the conservation of genetic diversity in African catfish (Clarias gariepinus).
Comparative Biochemistry and Physiology(Part A): Physiology,1993,106(2):313-318
|
被引
1
次
|
|
|
|
|
3.
Tiersch T R. Transport and cryopreservation of sperm of the common snook, Centropomus undecimalis (Bloch).
Aquaculture Research,2004,35(3):278-288
|
被引
1
次
|
|
|
|
|
4.
Tiersch T R.
Cryopreservation of sperm of the Endangered Razorback Sucker,2000:95-104
|
被引
1
次
|
|
|
|
|
5.
Ohta H. Cryopreservation of the sperm of the Japanese bitterling.
Journal of Fish Biology,2001,58(3):670-681
|
被引
3
次
|
|
|
|
|
6.
Blaxter J H S. Sperm storage and cross-fertilization of spring and autumn spawning herring.
Nature,1953,172:1189-1190
|
被引
16
次
|
|
|
|
|
7.
Gwo J C.
Cryopreservation of sperm of some marine fishes,2000:138-160
|
被引
1
次
|
|
|
|
|
8.
Liu Q. An efficient methodology for cryopreservation of spermatozoa of red seabream, Pagrus major, with 2-mL cryovials.
Journal of the World Aquaculture Society,2006,37(3):289-297
|
被引
2
次
|
|
|
|
|
9.
Liu Q H. Use of computer-assisted sperm analysis (CASA) to evaluate the quality of cryopreserved sperm in red seabream (Pagrus major).
Aquaculture,2007,263(1/4):20-25
|
被引
9
次
|
|
|
|
|
10.
Liu Q H. Flow cytometry and ultrastructure of cryopreserved red seabream (Pagrus major) sperm.
Theriogenology,2007,67(6):1168-1174
|
被引
7
次
|
|
|
|
|
11.
Liu Q H. Effect of storage time and cryoprotectant concentrations on the fertilization rate and hatching rate of cryopreserved sperm in red seabream (Pagrus major Temminck & Schlegel, 1843).
Aquaculture Research,2010,41(9):89-95
|
被引
1
次
|
|
|
|
|
12.
李纯. 黑鲷精子的超低温保存研究.
海洋科学,2001,25(12):1-4
|
被引
6
次
|
|
|
|
|
13.
叶霆. 黑鲷精子的超低温冻存及DNA损伤的SCGE检测.
动物学研究,2009,30(2):151-157
|
被引
17
次
|
|
|
|
|
14.
肖志忠. 大黄鱼精液的高效超低温保存.
海洋科学,2007,31(4):1-4
|
被引
7
次
|
|
|
|
|
15.
Chen S L. Cryopreservation of sperm from turbot (Scophthalmus maximus) and application to large-scale fertilization.
Aquaculture,2004,236(1/4):547-556
|
被引
26
次
|
|
|
|
|
16.
Chen S L. Cryopreservation of flounder (Paralichthys olivaceus) embryos by vitrification.
Theriogenology,2005,63(4):1207-1219
|
被引
8
次
|
|
|
|
|
17.
洪万树. 花鲈精子生理特性及其精液超低温冷冻保存.
海洋学报,1996,18:97-104
|
被引
23
次
|
|
|
|
|
18.
雷霁霖. 美洲黑石斑鱼的品种优势和养殖前景.
海洋水产研究,2007,28(5):110-115
|
被引
15
次
|
|
|
|
|
19.
DeGraaf J D. Production and storage of sperm from the black sea bass Centropristis striata L.
Aquaculture Research,2004,35(15):1457-1465
|
被引
2
次
|
|
|
|
|
20.
Fabbrocini A. Cryopreservation of seabream (Sparus aurate) spermatozoa.
Cryobiology,2000,40:46-53
|
被引
4
次
|
|
|
|
|
了解气象卫星更多信息，请访问