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Neural cell injury microenvironment induces neural differentiation of human umbilical cord mesenchymal stem cells

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文摘 This study aimed to investigate the neural differentiation of human umbilical cord mesenchymal stem cells (hUCMSCs) under the induction of injured neural cells. After in vitro isolation and culture, passage 5 hUCMSCs were used for experimentation. hUCMSCs were co-cultured with normal or Aβ1-40-injured PC12 cells, PC12 cell supernatant or PC12 cell lysate in a Transwell co-culture system. Western blot analysis and flow cytometry results showed that choline acetyltransferase and microtubule-associated protein 2, a specific marker for neural cells, were expressed in hUCMSCs under various culture conditions, and highest expression was observed in the hUCMSCs co-cultured with injured PC12 cells. Choline acetyltransferase and microtubule-associated protein 2 were not expressed in hUCMSCs cultured alone (no treatment). Cell Counting Kit-8 assay results showed that hUCMSCs under co-culture conditions promoted the proliferation of injured PC12 cells. These findings suggest that the microenvironment during neural tissue injury can effectively induce neural cell differentiation of hUCMSCs. These differentiated hUCMSCs likely accelerate the repair of injured neural cells.
来源 Neural Regeneration Research ,2012,7(34):2689-2697 【核心库】
DOI 10.3969/j.issn.1673-5374.2012.34.006
关键词 stem cell ; umbilical cord mesenchymal stem cell ; co-culture ; induction ; differentiation ; neural cell ; microtubule-associated protein 2 ; injured cell ; Transwell ; neural regeneration ; regeneration
地址

1. Department of Neurology, First People’s Hospital of Shenyang, Liaoning, Shenyang, 110041  

2. First Affiliated Hospital of China Medical University, Liaoning, Shenyang, 110001  

3. College of Basic Medical Sciences, China Medical University, Liaoning, Shenyang, 110001  

4. Liaoning Provincial Blood Center, Liaoning, Shenyang, 110044

语种 英文
ISSN 1673-5374
学科 基础医学
文献收藏号 CSCD:4747648

参考文献 共 63 共4页

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引证文献 2

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