一株抗肿瘤放线菌的鉴定及生物活性
Identification and Bioactivity of an Antitumor Actinomycete
查看参考文献22篇
文摘
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为开发青藏高原沙地生态系统放线菌资源,首次从位于青藏高原东北部的青海湖沙岛土壤样品中选择性分离培养出一株具明显抑菌及抗肿瘤活性放线菌Sd-31.通过生理生化特性、化学分类学以及16S rRNA序列分析鉴定,菌株为砖红链霉菌,命名为Streptomyces lateritius Sd-31;双层琼脂法检测菌株抑菌活性,表明菌株对金黄色葡萄球菌(Staphyloccocus aureus)以及枯草芽孢杆菌(Bacillus subtilis)有显著抑制作用;通过Hoechst染色荧光显微镜观察及MTT法细胞毒性试验,确定菌株发酵液有明显的诱导肿瘤细胞凋亡活性且与处理浓度成正相关;纸片法研究菌株发酵液活性稳定性,结果表明,20-80°C处理后发酵液抑菌活性稳定,100°C条件处理后发酵液抑菌活性降低;在酸性及中性条件下发酵液活性稳定,碱性条件下随着pH值的增加发酵液抑菌活性降低.以上结果为抗肿瘤药物的研究提供了理论数据.图6表4参23 |
其他语种文摘
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To exploit actinomycetes from desert ecosystem of Qinghai-Tibet plateau, an antimicrobial and antitumor actinomycete Sd-31 was selectively isolated for the first time from the soil sample of the desert island in the Qinghai Lake, the northeast of Qinghai-Tibet plateau. According to physio-biochemical, chemotaxonomy and 16S rRNA gene sequence analysis, the strain was identified and named Streptomyces lateritius Sd-31. The antimicrobial bioactivity of the strain was tested by double layer agar method, and the result showed that the strain could inhibit the growth of Staphyloccocus aureus and Bacillus subtilis. According to Hoechst fluorescence microscope observation and cytotoxic test by MTT, the broth of the strain showed obvious bioactivity of leading to the apotosis of tumor cell. The stability of the broth bioactivity was tested by paper disk method, and the result showed that the antimicrobial bioactivity was stable between 20℃ and 80℃ and decreased at 100 ℃. The antimicrobial bioactivity was stable at acid and neutral treatment and decreasing with the increasing of pH at alkaline treatment. These findings provided data for exploring antitumor drugs. Fig 6, Tab 4,Ref 23 |
来源
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应用与环境生物学报
,2012,18(6):983-987 【核心库】
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DOI
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10.3724/sp.j.1145.2012.00983
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关键词
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青藏高原
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青海湖沙岛
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放线菌
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砖红链霉菌
;
抗肿瘤
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菌株鉴定
;
生物活性
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地址
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1.
中国科学院两北高原生物研究所, 西宁, 810001
2.
华东理工大学, 生物反应器工程国家实验室, 上海, 200237
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语种
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中文 |
ISSN
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1006-687X |
学科
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微生物学 |
基金
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国家科技支撑计划项目
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文献收藏号
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CSCD:4744031
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参考文献 共
22
共2页
|
1.
Zhao K. The diversity and anti-microbial activity of endophytic actinomycetes isolated from medicinal plants in Panxi plateau, China.
Curr Microbiol,2011,62:182-190
|
被引
19
次
|
|
|
|
2.
Okoro C K. Diversity of culturable actinomycetes in hyper-arid soils of the Atacama Deserts, Chile.
Antonie Van Leeuwenhoek,2009,95:121-133
|
被引
8
次
|
|
|
|
3.
Jiang S M. Diversity of culturable actinobacteria isolated from maring sponge Haliclona sp.
Antonie Van Leeuwenhoek,2007,92(4):405-416
|
被引
5
次
|
|
|
|
4.
Zenova G M. The structural-functional organization of the thermotolerant complexes of actinomycetes in desert and volcanic soils.
Eurasian Soil Sci,2009,42(5):531-535
|
被引
1
次
|
|
|
|
5.
司美茹. 青海高原土壤拮抗性放线菌的生态分布.
应用与环境生物学报,2005,11(1):104-111
|
被引
15
次
|
|
|
|
6.
迪丽拜尔·托乎提. 新疆罗布泊外围地区极端环境嗜碱放线菌生物学特性及活性物质筛选.
应用与环境生物学报,2009,15(6):835-839
|
被引
3
次
|
|
|
|
7.
范丽霞. The effect of natural air-dry time on actinomycetes isolation from sample soil.
海南医学院学报,2010,16(3):280-284
|
被引
1
次
|
|
|
|
8.
姜怡. 稀有放线菌分离方法.
微生物学通报,2006,33(1):181-183
|
被引
38
次
|
|
|
|
9.
姜怡. 超声波处理土样分离放线菌.
微生物学报,2010,50(8):1094-1097
|
被引
6
次
|
|
|
|
10.
Staneck J L. Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography.
Appl Microbiol,1974,28(2):226-231
|
被引
17
次
|
|
|
|
11.
阮继生.
Rapid Identification and Systematics of Actinobacteria,2011:69-116
|
被引
1
次
|
|
|
|
12.
徐丽华.
Actinomycete Systematic-Principle, Methods and Practice,2007:80-87
|
被引
1
次
|
|
|
|
13.
Heuer H. Analysis of actinomycete communities by specific amplification of genes encoding 16S rRNA and gel-electrophoretic separation in denaturing gradients.
Appl Environ Microbiol,1997,63:3233-3241
|
被引
79
次
|
|
|
|
14.
Tamura K. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) Software Version 4.0.
Mol Biol Evol,2007,24(8):1596-1599
|
被引
2001
次
|
|
|
|
15.
Thompson J D. The clustal_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools.
Nucleic Acids Res,1997,25:4876-4882
|
被引
2393
次
|
|
|
|
16.
Chen J. Agromyces flavus sp. nov., an actinomycete isolated from soil.
Int J Syst Evol Microbiol,2011,61(7):1705-1709
|
被引
1
次
|
|
|
|
17.
Zhang Y Q. Diversity of culturable actinobacteria from Qinghai-Tibet Plateau, China.
Antonie Van Leeuwenhoek,2010,98:213-223
|
被引
7
次
|
|
|
|
18.
Zhang Y Q. Isoptericola halotolerans sp. nov., a novel actinobacterium isolated from saline soil from Qinghai Province, north- west China.
Int J Syst Evol Microbiol,2005,55:1867-1870
|
被引
5
次
|
|
|
|
19.
Zhang Y Q. Zhihengliuella halotolerans gen. nov., sp. nov., a novel member of the family Micrococcaceae.
Int J Syst Evol Microbiol,2007,57:1018-1023
|
被引
5
次
|
|
|
|
20.
Tuntiwachwuttikul P. secondary metabolites from Streptomyces sp. SUCI.
Tetrahedron,2008,54:7583-7586
|
被引
1
次
|
|
|
|
|