石榴花多酚对糖尿病合并脂肪肝大鼠肝脏中PON表达的影响
Effects of pomegranate flowers polyphenols on liver PON expression of diabetes combining non-alcoholic fat liver diseases rats
查看参考文献17篇
|
文摘
|
探讨石榴花多酚对糖尿病合并脂肪肝大鼠肝脏的影响及作用机制。采用高脂喂养联合腹腔注射链脲佐菌素(streptozocin, STZ)的方法建立糖尿病合并脂肪肝大鼠模型,将成模大鼠随机分为:模型组、二甲双胍组、石榴花多酚低、中、高剂量组(75、150和300mg·kg~(?1))。干预4周后处死大鼠,取血和肝脏,测定血清空腹血糖(fastingplasmaglucose, FPG)、血脂、胰岛素水平(fastinginsulin, FINS)和血清丙氨酸氨基转移酶(alanineaminotransferase, ALT)和天门冬氨酸氨基转移酶(aspartateaminotransferase, AST)水平;肝脏及血清超氧化物歧化酶(superoxidedismutase, SOD)和丙二醛(malondialdehyde, MDA)含量;肝脏组织对氧磷酶(paraoxonase, PON1和3)mRNA和蛋白表达,并观察肝脏的病理改变。石榴花多酚各剂量组大鼠血脂、胰岛素抵抗水平和转氨酶显著降低;血清高密度脂蛋白胆固醇含量升高;肝细胞内脂肪滴较模型组明显减少;抗氧化能力增强;肝组织PON1mRNA和蛋白表达水平显著增加。石榴花多酚可能通过增加肝组织中PON1mRNA和蛋白的表达,缓解胰岛素抵抗,增强机体抗氧化能力从而改善大鼠肝脏脂肪变性。 |
|
其他语种文摘
|
To investigate the protective effects and possible mechanism of pomegranate flowers polyphenols (PFP) on liver function of rats with diabetes combining non-alcoholic fat liver diseases, diabetes combining non- alcoholic fat liver disease model rats were established with high calorie feeding and small dose intraperitoneal injection of streptozotocin (STZ). Model rats were randomly divided into: model group, metformin group, pomegranate flowers polyphenols low, medium and high dose group (75, 150 and 300 mg·kg?1). After four weeks treatment, the levels of FPG, blood fat profiles and serum insulin, ALT, AST levels, SOD and MDA in the liver and serum separately were analyzed with biochemical methods. Paraoxonase (PON1 and PON3) mRNA and protein expression in liver were checked by RT-PCR and immunohistochemical method. Pathological changes of the liver were observed. FPG, IRI, non-HDL-C and transaminase significantly reduced and HDL-C raised in the each PFP dose group; Furthermore, compared with model group, fat drops in liver cells significantly reduced, antioxidant ability enhanced, PON1 mRNA and protein expression level in liver increased significantly. The protective effects of PFP against diabetes combining non-alcoholic fat liver diseases rats might through the increase liver PON1 mRNA and protein expression further enhanced the body antioxidant capacity and reduced IRI so as to ameliorate the rat hepatic steatosis. |
|
来源
|
药学学报
,2013,48(1):71-76 【核心库】
|
|
关键词
|
糖尿病合并脂肪肝
;
石榴花多酚
;
胰岛素抵抗
;
对氧磷酶
|
|
地址
|
1.
新疆医科大学生理学教研室, 新疆, 乌鲁木齐, 830011
2.
新疆医科大学药理学教研室, 新疆, 乌鲁木齐, 830011
3.
新疆医科大学中国科学院新疆理化技术研究所, 新疆, 乌鲁木齐, 830011
|
|
语种
|
中文 |
|
文献类型
|
研究性论文 |
|
ISSN
|
0513-4870 |
|
学科
|
药学 |
|
基金
|
新疆自然科学基金
;
国家自然科学基金资助项目
|
|
文献收藏号
|
CSCD:4724091
|
参考文献 共
17
共1页
|
|
1.
Sattar N. Elevated alanine aminotransferase predicts new-onset type 2 diabetes independently of classical risk factors, metabolic syndrome, and C-reactive protein in the west of Scotland coronary prevention study.
Diabetes,2004,53:2855-2860
|
被引
11
次
|
|
|
|
|
2.
De Alwis N M. Non-alcoholic fatty liver disease: the mist gradually clears.
J Hepatol,2008,48:104-112
|
被引
66
次
|
|
|
|
|
3.
Qiang G F. Effect of metformin in liver fibrosis of type 2 diabetes rats.
药学学报,2010,45:801-806
|
被引
1
次
|
|
|
|
|
4.
Li H. Impact of statins on liver function.
Chin J Cardiovasc Rev,2006,4:5523-5524
|
被引
1
次
|
|
|
|
|
5.
Wei Y Y. Effects of pomegranate flower extract (PFE) on glycometabolism of type 2 diabetes rats induced by STZ.
Chin J Hospit Pharm,2011,31:537-540
|
被引
1
次
|
|
|
|
|
6.
Li Y. Effects of pomegranate flower extract (PFE) on FPG lipid profiles and liver morphosis of type 2 diabetes rats.
新疆医科大学学报,2010,33:379-382
|
被引
1
次
|
|
|
|
|
7.
Aisa H A.
Preparation methods and application polyphenol from Punica granatum Linn: CN, ZL 200810072910.5,2008
|
被引
1
次
|
|
|
|
|
8.
Li G W. A new index in detecing of insulin sensitivity.
中华内科杂志,1993,32:652
|
被引
1
次
|
|
|
|
|
9.
. The Chinese medical association hepatology branch fatty liver and alcoholic liver disease committee, Nonalcoholic fatty liver disease diagnosis and treatment guidelines.
中华肝脏病杂志,2010,18:163-166
|
被引
4
次
|
|
|
|
|
10.
Oe K. Nonalcoholic steatohepatitis (NASH) and nonalcoholic fatty liver disease (NAFLD).
Nihon Rinsho,2011,69(Suppl 1):406-409
|
被引
1
次
|
|
|
|
|
11.
Fridlyand L E. Does the glucose-dependent insulin secretion mechanism itself cause oxidative stress in pancreatic beta-cells?.
Diabetes,2004,53:1942-1948
|
被引
6
次
|
|
|
|
|
12.
Durrington P N. Paraoxonase and atherosclerosis.
Arterioscler Thromb Vasc Biol,2001,21:473-480
|
被引
16
次
|
|
|
|
|
13.
Van himbergen T M. The story of PON1: how an organ phosphate hydrolysing enzyme is becoming a player in cardiovascular medicine.
Neth J Med,2006,64:34-38
|
被引
4
次
|
|
|
|
|
14.
Harel M. 3-D structure of serum paraoxonase 1 sheds light on its activity, stability, solubility and crystallizability.
Arh Hig Rada Toksikol,2007,58:347-353
|
被引
1
次
|
|
|
|
|
15.
Ferre N. Hepatic paraoxonase activity alterations and free radical production in rats with experimental cirrhosis.
Metabolism,2001,50:997-1000
|
被引
2
次
|
|
|
|
|
16.
Gaidukov. The 192 R/Q polymorphs of serum paraoxonase PON1 differ in HDL binding, lipolactonase stimulation, and cholesterol efflux.
J Lipid Res,2006,47:2492-2502
|
被引
1
次
|
|
|
|
|
17.
Qu S S. Study on antioxidant activity of Uighur medicine pomegranate flower polyphenols.
中国医药学报,2012:27
|
被引
1
次
|
|
|
|
|
了解气象卫星更多信息，请访问