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温度刺激对斑马鱼仔鱼基因转录表达的影响
MICROARRAY ANALYSIS OF TEMPERATURE STRESS EFFECTS ON TRANSCRIPTIONAL EXPRESSION IN ZEBRAFISH LARVAE

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李林春 1   李青 2   龙勇 2   崔宗斌 2 *  
文摘 许多优良鱼类养殖品种不耐低温或高温的特点给水产养殖业带来诸多限制和困难,这些鱼类在胚胎和仔鱼等早期阶段的抗寒和抗热能力比成体更差,育苗过程中很容易受到温度突然变化的影响。虽然目前利用基因芯片技术已研究了温度刺激对几种鱼类成体组织中基因表达的影响,但温度刺激对仔鱼基因转录表达的影响还未见报道。研究以斑马鱼受精后96h的出膜仔鱼为实验材料,分别在低温(16℃)和高温(34℃)条件下处理12h和24h,用基因芯片技术检测温度刺激对其基因表达的影响。与培养在28℃的对照相比,低温和高温处理后共有3633个基因发生差异表达,其中低温处理后差异表达基因数目多于高温处理,而且低温抑制基因数目多于诱导表达基因的数目。生物信息学分析结果表明,低温诱导基因主要参与RNA加工和核糖体生物发生等生物学过程,高温诱导基因则主要参与应激反应和未折叠蛋白结合。低温抑制基因主要参与蛋白质水解、视觉感知以及铁离子结合等生物学功能,高温抑制基因参与的生物学功能包括DNA复制、神经系统过程和类固醇激素生物合成等。除了已报道的温度刺激响应基因外,研究鉴定出了大量尚未报道与温度刺激相关的基因,如参与RNA加工的rnmtl1a和pus3基因,以及参与转录调控的twistnb和aebp2基因等。研究结果为进一步揭示鱼类冷或热适应的分子机理和培养耐寒或耐热的养殖新品种提供理论基础。
其他语种文摘 The poor resistance of many important cultured fish species to low or high temperature brings many restrictions and difficulties to aquaculture.Since fish is more sensitive to cold or heat stress at embryo and larva stages,fish seedling breeding is often badly affected by abrupt temperature fluctuations.Although gene expression in mature tissues of several fish species exposed to temperature stress has been characterized by microarray analysis,the effects of temperature stress on gene expression in fish larvae has not been reported.In order to investigate the transcriptional response of fish to temperature stress at larval stage,the hatched zebrafish larvae at 96 hours after fertilization(hpf) were exposed to cold(16℃) or heat(34℃) stress for 12 and 24 hours respectively,and the expression of genes was detected by microarray analysis.The design of this study was aimed to identify common stress response genes regulated by both cold and heat stress and cold or heat stress specific genes.When compared to the controls maintained at 28℃,a total of 3633 genes were identified to be differentially expressed under temperature stress exposure.Cold stress regulated more genes than heat stress at both time points and the number of cold-inhibited genes was larger than that of cold-induced genes.Only a small part of these differentially expressed genes were regulated by both cold and heat stress.The results of bioinformatic analyses indicate that cold induced genes are mainly involved in biological processes including RNA processing and ribosome biogenesis;however,genes induced by heat stress are mainly associated with stress response and unfolded protein folding.Genes down-regulated by cold stress mainly participate in biological functions such as proteolysis,visual perception and iron ion binding,while heat-inhibited genes are mainly involved in biological processes including DNA replication,neurological system process and steroid biosynthesis.In addition to genes previously reported to be regulated by temperature stress,this study identified many cold-or heat-stress responsive genes,such as those involved in RNA processing,rnmtl1a(RNA methyltransferase like 1a) and pus3(pseudouridylate synthase 3);and genes associated with transcriptional regulation,twistnb(TWIST neighbor) and aebp2(AE binding protein 2).It could be concluded that a large number of genes and biological processes were regulated to establish the acclimated physiological status upon the exposure of fish to cold or heat stress.In-depth investigations on expression regulation and biological functions of genes specifically induced by cold or heat stress would further reveal molecular mechanisms underlying the cold or heat acclimation of fish and provide theoretical fundamentals for breeding cultured fish strains with cold-or heat-resistant ability.
来源 水生生物学报 ,2012,36(5):882-891 【核心库】
关键词 温度刺激 ; 斑马鱼 ; 基因表达 ; 基因芯片
地址

1. 厦门海洋职业技术学院, 厦门, 361012  

2. 中国科学院水生生物研究所, 武汉, 430072

语种 中文
ISSN 1000-3207
学科 分子生物学
基金 国家863计划
文献收藏号 CSCD:4646021

参考文献 共 39 共2页

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引证文献 6

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2 王倩 斑马鱼GSTM单核苷酸多态性与低温耐受性的相关分析 海洋科学,2015,39(1):1-7
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