饲喂不同浓度黄曲霉毒素B_1饲料对异育银鲫成鱼的生长和毒素积累的影响
GROWTH AND AFLATOXIN B_1ACCUMULATION OF GIBEL CARP ADULT FED WITH DIETS OF DIFFERENT LEVELS OF AFLATOXIN B_1
查看参考文献35篇
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文摘
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以含不同浓度黄曲霉毒素B_1(AFB_1)的配合饲料饲喂异育银鲫(Carassius auratus gibelio)成鱼56d,研究异育银鲫成鱼[(122.3±0.7)g]生长、生理反应、肝脏组织学变化、卵巢发育以及鱼体各组织中的AFB1的毒素积累状况。实验分为5个实验组,不同实验组饲料中AFB_1含量分别为0、5、20、50、500μg/kg饲料(实测值分别为2.59、4.12、12.39、46.23、454.07μg/kg饲料),每个处理3个平行。在整个实验过程中各实验组均未表现出外部形态和行为异常,各组存活率均达到100%。各实验组异育银鲫成鱼终末体重、摄食率(FR)、特定生长率(SGR)和饲料效率(FE)均无显著差异。饲料AFB_1水平对异育银鲫血清总胆固醇(TC)含量、血清谷丙转氨酶(GPT)、谷草转氨酶(GOT)和碱性磷酸酶(AKP)活性均无显著影响。各毒素组血清超氧化物岐化酶(SOD)活性与对照无显著差异。各毒素组肝脏和卵巢均未见明显的组织学病理变化。肌肉和性腺中的AFB_1积累量低于FDA食品安全限定标准(5μg/kg)。肝胰脏中的AFB_1积累和饲料中的AFB_1水平呈对数关系。饲喂AFB_1≥50μg/kg饲料使异育银鲫成鱼肝脏AFB_1积累超过安全限量标准。结果表明,异育银鲫成鱼至少可耐受AFB_1含量达500μg/kg饲料(实测值:454.07μg/kg饲料)56d。 |
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其他语种文摘
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A 56-day feeding trial was conducted to evaluate the effect of dietary aflatoxin B_1(AFB_1) on growth,physiological responses,histological changes,and accumulation in gibel carp(Carassius auratus gibelio) adult.Triplicate groups of gibel carp [(122.3 ± 0.7) g] were fed with five semi-purified diets(Diet 1 to 5) containing0,5,20,50 and 500μg/kg AFB(determined level was 2.59,4.12,12.39,46.23 and 454.07μg AFB_1/kg diet,respectively).During the experiment,photoperiod was 12D∶12L with the light period from 09:00 to 21:00,dissolved oxygen was above 6mg/L,ammonia-N was less than 0.4mg/L and pH was about 6.8.The results showed that during the 56-day of AFB_1 exposure,no external changes and unusual behavior were observed in the fish fed with various levels of AFB_1.The survival rate in all groups attained 100%.There was no significant difference in final body weight,feeding rate(FR),specific growth rate(SGR) and feed efficiency(FE) between the control and the other groups.Fish fed with various levels of AFB1 showed no significant differences in total cholesterol,activities of serum alanine aminotransferase(ALT),asparitic aminotransferase(AST),alkaline phosphatase(ALP) and superoxide dismutase(SOD) compared to the control group.No significant histological lesions in hepatopancreasand ovary were identified between the control and increasing AFB1 treatments.Low AFB_1 residues were found in muscles and ovary,and were below the safety limitation of 5μg/kg.Accumulation of AFB_1 in hepatopancreas was logarithmically correlated to the dietary AFB_1 level.Our results indicated that gibel carp was a less susceptible species to AFB_1 exposure up to approximately 500μg/kg diet(determined level was 454.07μg/kg diet),at least for 56 days. |
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来源
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水生生物学报
,2012,36(5):817-825 【核心库】
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关键词
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黄曲霉毒素B_1
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异育银鲫
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生长
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毒素积累
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地址
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1.
中国科学院水生生物研究所, 武汉, 430072
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中国科学院水生生物研究所, 水生生物多样性与保护国家重点实验室, 武汉, 430072
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语种
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中文 |
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ISSN
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1000-3207 |
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学科
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水产、渔业 |
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基金
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国家公益性行业（农业）科研专项
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国家公益性行业（农业）科研专项
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国家现代农业产业技术体系建设专项资金项目
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中国科学院知识创新工程青年人才领域前沿项目
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文献收藏号
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CSCD:4646013
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