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水华蓝藻噬藻体对不同条件培养的宿主细胞感染性分析
ANALYSIS OF THE CYANOPHAGE (PAV-LD) INFECTION IN HOST CYANOBACTERIA UNDER DIFFERENT CULTURE CONDITIONS

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文摘 在从武汉东湖水样中培养分离水华蓝藻噬藻体(Planktothrix agardhii Virus from Lake Donghu,PaV-LD)的基础上,对在不同条件培养的宿主蓝藻细胞中,PaV-LD增殖效率及裂解作用进行了测定分析。分别将PaV-LD接种到生长期、半连续培养更新率或光照不同的宿主蓝藻液中,并采用稀释培养计数(Mostprobable number,MPN)方法与电镜观察,测定子代PaV-LD释放量及宿主细胞的裂解作用。结果显示:对数生长期宿主蓝藻单个细胞中子代PaV-LD的平均释放量为350感染单位(Infectious Units,IU/cell),显著高于稳定生长期的平均释放量110 IU/cell。在用新鲜培养基更新率为0%、35%、50%和65%的半连续培养宿主蓝藻中,接种PaV-LD 5d之后,噬藻体的释放量分别约为50 IU/cell、70 IU/cell、220 IU/cell或310 IU/cell,表明子代PaV-LD释放率随培养基更新率的增加而显著提高。在光照条件下感染3—4d后,宿主蓝藻细胞充分裂解,并释放大量子代PaV-LD,滴度可由初始7.00×103IU/mL快速增加到8.56×107IU/mL;但在遮光条件下,同样感染的蓝藻细胞未见裂解,也检测不到释放的子代噬藻体。电镜观察显示,在光照条件下感染的蓝藻细胞类囊体膜结构消失,而大量子代PaV-LD颗粒主要分布在原有类囊体的部位。显然,宿主蓝藻细胞的培养条件和状态可能对获得噬藻体纯培养有决定性影响。
其他语种文摘 Based on a cyanophage PaV-LD(Planktohtrix agardhii Virus isolated from Lake Donghu) infecting bloom-forming filamentous cyanobacterium Planktothrix agardhii from a typical shallow freshwater lake—Lake Donghu,the impact of host cyanobacterial culture conditions on the cyanophage efficiency and lytic activity of this cyanophage was analyzed in this study.PaV-LD was inoculated into cyanobacterial cultures of host under different states,such as growth phase,renewal rate of semicontinuous cultures and illumination condition,and the burst size of progeny cyanophage and host cell lysis efficiency of host cyanobacteria was determined by the most-probable number(MPN) method and transmission electron microscopy(TEM) observation,respectively.The results showed that the average burst size of infectious progeny PaV-LD released from each infected host cell at exponential phase was 350 infectious units(IU)/cell,which was significantly higher than that of each infected host cell at stationary phase(110 IU/cell);the initial cell densities of host cultures at exponential phase and stationary phase was 1.38×106 cells/mL and 1.43×107 cells/mL,and respectively decreased to 1.75×103cells/mL and 3.0×103 cells/mL at 5 days after infection,indicating that no significant difference was found in the sensitivity of host cell at different growth stage to PaV-LD infection.Semicontinuous culture experiment was performed,either 0,35%,50% and 65% of the fresh BG11 medium was replaced and 5 days after infection,the burst size of PaV-LD were estimated to be ca.50 IU/cell,70 IU/cell,220 IU/cell and 310 IU/cell.Thus,the burst size of PaV-LD increased with the addition of medium renewal rate in a certain range.Infected host cells were lysed under light 3 or 4 days later.Numerous progeny PaV-LD was observed with TEM.Additionally,the titer aggrandized rapidly from 7.0×103 IU/mL to 8.56×107 IU/mL during this period.On the contrary,the infected host cells were not cracked under darkness.Simultaneously,progeny PaV-LD could not be detected.Further,TEM observation showed that thylakoid membranes of infected host cells under light were disappeared,and progeny PaV-LD were mainly distributed in the thylakoid location of the original.The results showed that cyanobacteria cell culture conditions might play a decisive role in obtaining pure cultures of the cyanophage
来源 水生生物学报 ,2012,36(3):420-425 【核心库】
关键词 丝状蓝藻病毒(PaV-LD) ; 噬藻体感染性 ; 蓝藻细胞裂解 ; 半连续培养 ; 稀释培养计数(MPN)
地址

中国科学院水生生物研究所, 淡水生态与生物技术国家重点实验室, 武汉, 430072

语种 中文
文献类型 研究性论文
ISSN 1000-3207
学科 微生物学
基金 中国科学院先导专项项目 ;  中国科学院水生生物研究所淡水生态与生物技术国家重点实验室基金
文献收藏号 CSCD:4545272

参考文献 共 24 共2页

1.  Sullivan M B. Cyanophages infecting the oceanic cyanobacterium Prochlorococcus. Nature,2003,424:1047-1051 被引 26    
2.  Garza D R. The effect of cyanophages on the mortality of Synechoeoccus spp. and selection for UV resistant viral communities. Microbial Ecology,1998,36:281-292 被引 9    
3.  Hewson I. Virus-like particles associated with Lyngbya majuscula (Cyanophyta; Oscillatoriacea) bloom decline in 5 Moreton Bay, Australia. Aquatic Microbial Ecology,2001,25:207-213 被引 7    
4.  Kao C C. Diel infection of a cyanobacterium by a contractile bacteriophage. Applied Environmental Microbiology,2005,71:4276-4279 被引 3    
5.  张奇亚. 一类不可忽视的战略生物资源-淡水与海水中的病毒及其在生态系统中的作用. 中国科学院院刊,2009,24(4):414-420 被引 14    
6.  Proctor L M. Viral mortality of marine bacteria and cyanobacteria. Nature,1990,343:60-62 被引 49    
7.  Fuhrman J A. Marine viruses and their biogeochemical and ecological effects. Nature,1999,399:541-547 被引 120    
8.  Liu Y M. Seasonal variation of virioplankton in a eutrophic shallow lake. Hydrobiologia,2006,560:323-334 被引 14    
9.  张奇亚. 浮游病毒. 水生生物学报,2002,26(6):691-696 被引 14    
10.  刘艳鸣. 武汉东湖浮游病毒的丰度及多样性. 水生生物学报,2005,29(1):1-6 被引 13    
11.  刘艳鸣. 利用脉冲场凝胶电泳测定东湖浮游病毒基因组的大小. 武汉大学学报(理学版),2005,51(S2):238-240 被引 10    
12.  Corinaldesi C. Large-scale spatial distribution of virioplankton in the Adriatic Sea: testing the trophic state control hypothesis. Applied and Environmental Microbiology,2003,69:2664-2673 被引 9    
13.  Stoddard L I. Selection and characterization of cyanophage resistance in marine Synechococcus strains. Applied and Environmental Microbiology,2007,73:5516-25522 被引 7    
14.  Wommack K E. Virioplankton: viruses in aquatic ecosystems. Microbiology and Molecular Biology Reviews,2000,64:69-114 被引 95    
15.  Bailey S. Cyanophage infection and photoinhibition in marine cyanobacteria. Research in Microbiology,2004,155:720-725 被引 9    
16.  Gao E B. Isolation of a novel cyanophage infectious to filamentous cyanobacterium Planktothrix agardhii (cyanophyceae) from Lake Donghu in China. Aquatic Microbial Ecology,2009,54:163-170 被引 13    
17.  Saitoh S. Development of a mostprobable-number method for enumerating denitrifying bacteria by using 96-well microtiter plates and an anaerobial culture system. Microbes Environments,2003,18:210-215 被引 1    
18.  Oliver J D. The viable but nonculturable state in bacteria. Journal of Microbiology,2005,43(No. S):93-100 被引 53    
19.  Oliver J D. Recent findings on the viable but nonculturable state in pathogenic bacteria. FEMS Microbiology Reviews,2010,34:415-425 被引 46    
20.  Liu Y M. Spatial distribution and morphologic diversity of virioplankton in Lake Donghu, China. Acta Oecologica,2006,29:328-334 被引 18    
引证文献 6

1 李三华 噬藻体PaV-LD主要衣壳蛋白、穿孔素和内肽酶基因的克隆及表达分析 水生生物学报,2013,37(2):252-260
被引 4

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