水生动物病毒的电镜和荧光显微镜观察
Observations of aquatic animal viruses by electron microscopy and fluorescence microscopy
查看参考文献13篇
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文摘
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应用透射电镜和荧光显微镜对三种水生动物病毒粒子形态、病毒感染细胞的超微结构变化及亚细胞定位进行比较和研究。负染电镜观察显示:鳜鱼弹状病毒(SCRV)粒子呈典型的子弹状形态,长约76~118 nm,直径约29~52 nm;鲈鱼呼肠孤病毒(LJRV)粒子呈正二十面体结构,具有双层衣壳,直径约70~80 nm;蛙虹彩病毒(RGV)粒子呈正二十面体结构,具有囊膜,直径大小约150 nm。超薄切片观察表明,宿主细胞的基本结构遭到不同程度的破坏,成熟病毒粒子分布在胞质中或以出芽的方式释放到胞外,且RGV增殖后在胞质中聚集形成晶格状结构。免疫荧光观察进一步显示,RGV感染细胞后能产生多个直径大小不一的包涵体。本研究结果有助于了解和认识水生动物病毒的超微形态特征、复制过程及致病机理。 |
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其他语种文摘
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Electron microscope and fluorescence microscope were used to observe and study three aquatic animal viruses,ultra-structural changes in infected cells and subcellular localization of viral proteins in fish cells.The negatively stained electron micrographs revealed that Siniperca chuatsi rhabdovirus(SCRV) has typical bullet-shape and the size ranges from 76 ~ 118 nm in length and 29 ~ 52 nm in diameter.Lateolabrax japonicus reovirus(LJRV) has icosahedral structure with a double-layered protein capsid,and the size is 70~80 nm in diameter.Rana grylio virus(RGV) is icosahedral in appearance with diameter of about 150 nm and composed of envelope.The electron micrographs of the ultra-thin section of infected cells suggested that the basic structures of infected cells had been damaged to a different extent,and the mature virus particles were distributed in the cytoplasm or released into the extra-cellular by budding.Moreover,paracrystalline arrays of RGV virus particles were observed in the cytoplasm of infected-cells.In addition,viral inclusion bodies were detected in RGV-infected cells by fluorescence assay.These results may help to understand the ultrastructural features,replication,and pathogenesis of different aquatic viruses. |
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来源
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电子显微学报
,2012,31(2):190-193 【核心库】
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关键词
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水生动物病毒
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超微结构变化
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亚细胞定位
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地址
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中国科学院水生生物研究所, 淡水生态与生物技术国家重点实验室, 湖北, 武汉, 430072
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语种
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中文 |
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文献类型
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研究性论文 |
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ISSN
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1000-6281 |
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学科
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遗传学;动物学;畜牧、动物医学、狩猎、蚕、蜂 |
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基金
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国家自然科学基金资助项目
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中国科学院水生生物研究所淡水生态与生物技术国家重点实验室基金
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文献收藏号
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CSCD:4535846
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