维吾尔药毛菊苣提取物降糖活性的研究
Hypoglycemic Effect of Extracts from Cichorium glandulosum
查看参考文献18篇
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文摘
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本文利用糖尿病及其并发症治疗药物筛选中的关键靶点:PTP1B、α-葡萄糖苷酶和蛋白非酶糖化过程,对维吾尔药毛菊苣中提取分离后获得11个标准提取物进行降糖活性成分筛选。结果表明它们都具有PTP1B抑制剂作用,其中活性最好的组分IC_(50)为5.8±0.15μg/mL,五种毛菊苣根提取物和一种毛菊苣籽(CGS-1)提取物具有α-葡萄糖苷酶抑制活力,仅CGS-1具有一定的抑制蛋白非酶糖化过程的能力,其他组分均未见此活力,最后在转染的CHO细胞上对CGS-1作用机理进行了初步探索,观察到CGS-1可使磷酸化AKT蓄积,提示该组分可能通过PI3K/AKT途径刺激GLU4的转运从而达到降糖的目的。 |
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其他语种文摘
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In this paper,eleven components of Cichorium glandulosum Boiss et Hout were prepared,and their hypoglycemic effects were tested by using protein tyrosine phosphatase 1B(PTP1B),α-glucosidase,and AGE-BSA Non-enzyme Glycolysis inhibitor assay methods.The results showed us that all of the extracts are PTP1B inhibitors,and IC_(50) of the most active one is only 5.8±0.15 μg/mL Five of them are α-glucosidase inhibitor,and only CGS-1 is an AGE-BSA Non-enzyme Glycolysis inhibitor.Then,the mechanism of CGS-1 was investigated on CHO cell line which transiently transfected with pJ3H-PTP1B,and accumulated phosphor-AKT were observed in the cell,which may indicate that this is the pathway for anti-diabetes. |
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来源
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天然产物研究与开发
,2012,24(2):234-238 【核心库】
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关键词
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毛菊苣
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降糖
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蛋白酪氨酸酶1B
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α-葡萄糖苷酶
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蛋白非酶糖化
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地址
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中国科学院新疆理化技术研究所, 中国科学院干旱区植物资源化学重点实验室;;新疆特有药用资源利用实验室, 乌鲁木齐, 830011
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语种
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中文 |
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ISSN
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1001-6880 |
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学科
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中国医学 |
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基金
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中国科学院、国家外国专家局创新团队国际合作伙伴计划项目
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新疆科技重大专项课题
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国家自然科学基金国家杰出青年科学基金
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文献收藏号
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CSCD:4467477
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参考文献 共
18
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