气候条件和作物对黑土和潮土固氮微生物群落多样性的影响
IMPACTS OF CLIMATE AND CROPPING ON COMMUNITY DIVERSITY OF DIAZOTROPHS IN PACHIC UDIC ARGIBOROLL AND FLUVENTIC USTOCHREPT
查看参考文献30篇
文摘
|
固氮微生物是土壤氮素的主要贡献者之一。利用在黑龙江海伦(寒温带)、河南封丘(暖温带)和江西鹰潭(中亚热带)设置的2种农田土壤(黑土、潮土)的置换试验,研究了不同气候、土壤和种植条件对固氮微生物多样性的影响。通过直接从土壤中提取DNA,对固氮酶基因nifH PCR扩增并进行DGGE电泳的分析方法研究了2种类型土壤在3种不同水热条件下固氮微生物群落多样性的变化特征。研究结果表明,在置换到不同气候条件下3年后,土壤类型是决定固氮微生物结构及多样性的主要影响因子,其次是短期气候条件变化的影响,最后是种植玉米的影响。土壤固氮微生物多样性、优势度与土壤C/N及碱解氮含量呈显著正相关(p<0.01),与pH呈显著负相关;多元回归分析及典范对应分析均表明土壤碱解氮含量是影响固氮微生物多样性的决定因素。水热条件与土壤固氮微生物多样性没有线性相关关系,暖温带条件下黑土固氮微生物多样性最高,而潮土最低。种植玉米提高了土壤固氮微生物多样性。 |
其他语种文摘
|
In agricultural soils,besides anthropogenic sources,diazotrophs are the main source of nitrogen.Effects of temporary climate disturbance and cropping of maize on composition and diversity of nitrogen fixing bacteria in Pachic Udic Argiboroll and Fluventic Ustochrept were examined with the technique of nifH-gene targeted polymerase chain reaction(PCR)-denaturing gradient gel electrophoresis(DGGE) approach combined with advanced statistical analysis,based on soil reciprocal transplantation experiments which were set up along a latitude gradient with distinctly different temperature and precipitation.Both cluster analysis and canonical correspondence analysis(CCA) show that soil properties could be the dominant factor to stimulate variation of diazotrophic composition,whereas temporary climate disturbances also caused a certain extent of differences.Contents of organic carbon and alkali-hydrolyzable N were found to be in significantly positive correlation with Shannon and Simpson indices while pH was in significantly negative correlation.Statistical analysis show that the content of alkali-hydrolyzable N was the determinant variable which explained the differences in diazotrophic composition among all samples(p=0.002).Hydrothermal conditions didn’t have any linear correlation with diazotrophic biodiversity.However,Pachic Udic Argiboroll showed the highest diazotrophic biodiversity while Fluventic Ustochrept showed the lowest under warm temperate climate.In addition,maize plantation increased slightly the diversity of nitrogen-fixing bacterial in all soils,except for in Fluventic Ustochrept in Hailun. |
来源
|
土壤学报
,2012,49(1):130-138 【核心库】
|
关键词
|
nifH基因
;
微生物多样性
;
DGGE
;
气候
;
土壤类型
;
玉米
|
地址
|
1.
中国科学院南京土壤研究所, 土壤与农业可持续发展国家重点实验室, 南京, 210008
2.
扬州大学资源与环境学院, 江苏, 扬州, 225009
3.
中国科学院东北地理与农业生态研究所, 哈尔滨, 150040
|
语种
|
中文 |
文献类型
|
研究性论文 |
ISSN
|
0564-3929 |
学科
|
农业基础科学 |
基金
|
国家自然科学基金项目
;
国家973计划
;
中国科学院知识创新工程重要方向项目
|
文献收藏号
|
CSCD:4451955
|
参考文献 共
30
共2页
|
1.
王绍武. 近百年全球气候变暖的分析.
大气科学,1995,19(5):545-553
|
被引
137
次
|
|
|
|
2.
孙波. 水热条件与土壤性质对农田土壤硝化作用的影响.
环境科学,2009,30(1):206-213
|
被引
25
次
|
|
|
|
3.
Young J P W. Biological nitrogen fixation, phylogenic classification of nitrogen-fixing organisms.
Chapman and Hall,1992:43-86
|
被引
1
次
|
|
|
|
4.
申卫收. 绰墩山遗址古水稻土的一些微生物学特性研究.
土壤学报,2006,43(5):814-820
|
被引
7
次
|
|
|
|
5.
罗青. PCR—DGGE法研究福建省稻田土壤微生物地区多态性.
中国生态农业学报,2008,16(3):669-674
|
被引
12
次
|
|
|
|
6.
鲁如坤.
土壤农业化学分析方法,1999
|
被引
1973
次
|
|
|
|
7.
Poly F. Improvement in RFLP procedure to study the community of nitrogen fixers in soil through the diversity of nifH gene.
Research in Microbiology,2001,152:95-103
|
被引
52
次
|
|
|
|
8.
Gauch H G.
Multivariate analysis in community ecology,1982
|
被引
35
次
|
|
|
|
9.
Terbraak C J F. Canonical correspondence analysis and related multivariate methods in aquatic ecology.
Aquatic Sciences,1995,57(3):255-289
|
被引
58
次
|
|
|
|
10.
Mergel A. Relative abundance of denitrifying and dinitrogen-fixing bacteria in layers of a forest soil.
FEMS Microbiology Ecology,2001,36(1):33-42
|
被引
20
次
|
|
|
|
11.
Zehr J P. Use of degenerate oligonucleotides for amplification of the nifH gene from the marine cyanobacterium Trichodesmium thiebautii.
Applied and Environmental Microbiology,1989,55(10):2522-2526
|
被引
25
次
|
|
|
|
12.
Atlas R M.
Microbial ecology, fundamentals and applications,1981:125
|
被引
2
次
|
|
|
|
13.
Dommergues Y.
Soil microbial ecology,1970:56-57
|
被引
3
次
|
|
|
|
14.
Staddon W J. Soil microbial diversity and community structure across a climatic gradient in western Canada.
Biodiversity and Conservation,1998,7(8):1081-1092
|
被引
25
次
|
|
|
|
15.
Teng Q H. Analysis of nifH gene diversity in red soil amended with manure in Jiangxi, South China.
The Journal of Microbiology,2009,47(1):1-7
|
被引
2
次
|
|
|
|
16.
Keeling A A. Effects of carbohydrate application on diazotroph populations and nitrogen availability in grass swards established in garden waste compost.
Bioresource Technology,1998,66(2):89-97
|
被引
10
次
|
|
|
|
17.
Limmer C. Non-symbiotic N_2 fixation in acidic and pH-neutral forest soil: Aerobic and anaerobic differentials.
Soil Biology and Biochemistry,1996,28(2):177-183
|
被引
7
次
|
|
|
|
18.
张于光. 三江源高寒草甸土固氮基因(nifH)的多样性和系统发育研究.
微生物学报,2005,45(2):166-171
|
被引
19
次
|
|
|
|
19.
Montanez A. Biological nitrogen fixation in maize (Zea mays L.) by ~(15)N isotope-dilution and identification of associated culturable diazotrophs.
Biology and Fertility of Soils,2009,45(3):253-263
|
被引
2
次
|
|
|
|
20.
Dasilva K R A. Application of a novel Paenibacillus-specific PCR-DGGE method and sequence analysis to assess the diversity of Paenibacillus spp. in the maize rhizosphere.
Journal of Microbiological Methods,2003,54(2):213-231
|
被引
4
次
|
|
|
|
|