若尔盖高原湿地藻类多样性研究
Research on Algal Diversity in Zoige Alpine Wetland
查看参考文献25篇
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文摘
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分别于夏、冬两季采集若尔盖高原湿地水样,利用传统培养及显微镜检的方法和现代分子生物学方法对其藻类多样性进行了研究。结果表明,夏季水样至少培养出4种绿藻,分别为普通小球藻(Chlorella vulgaris Beij.)、浮球藻(Planktosphaeriagelatinosa G.M.Smith)、繁茂栅列藻(Scenedesmus abundans Kirchner)和柱状栅列藻(Scenedesmus bijuga Turp.),均属于绿球藻(Chlorococcales),其中普通小球藻为优势藻种,并被分离纯化.显微镜检结果表明冬季水样中主要的藻类种群为硅藻,此结果符合在低温季节天然水体中常以硅藻为主的常理.蓝藻16S rRNA基因克隆文库中的主要类群为硅藻(Bacillaripiophyta,35.3%),其次为蓝藻,包括色球藻(Chroococcales,5.9%)、念珠藻(Nostocales,11.8%)、颤藻(Oscillatoriales,11.8%)和一些未知种属的蓝藻类群(Unclassified Cyanobacteria,35.3%),还发现一些与低温环境微生物相关的类群,如颤藻目细纤菌属(Leptolyngbya). |
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其他语种文摘
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Water samples were collected in summer and winter in Zoige Alpine Wetland.Both the traditional cultivable and microscopic method and modern molecular approach were used to reveal the algal diversity in the wetland.For the samples collected in summer,single-cell Chlorophyta was cultured,isolated and identified.The results showed that there were at least four kinds of Chlorophyta,which were Chlorella vulgaris Beij.,Planktosphaeria gelatinosa G.M.Smith,Scenedesmus abundans Kirchne and Scenedesmus bijuga Turp.They all belonged to Chlorococcales.The dominant species was Chlorella vulgaris Beij.,which had been purified.For the samples collected in winter,the microscopic results showed that the dominant populations were diatoms,which is in accordance with the principle that the dominant populations of algae are diatoms in low-temperature seasons.Cyanobacterial 16S rRNA gene clone library showed that the dominant group was Bacillaripiophyta(35.3%).And Cyanobacteria contained the orders of Chroococcales(5.9%),Nostocales(11.8%),Osillatoriales(11.8%) and Unclassified Cyanobacteria(35.3%).There were some clones related to those from frigid habitats,such as Leptolyngbya-related clones. |
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来源
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环境科学
,2012,33(3):979-986 【核心库】
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关键词
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若尔盖高原湿地
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藻类多样性
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显微镜检
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克隆文库
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低温
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地址
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中国科学院研究生院, 北京, 100049
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语种
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中文 |
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文献类型
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研究性论文 |
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ISSN
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0250-3301 |
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学科
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环境科学基础理论 |
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基金
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中国科学院知识创新工程重要方向项目
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中国科学院战略性先导科技专项
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中国科学院研究生院院长基金
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文献收藏号
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CSCD:4451172
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参考文献 共
25
共2页
|
|
1.
张晓君.
青藏高原微生物多样性及其与环境的关系,2002
|
被引
1
次
|
|
|
|
|
2.
何剑锋. 北极拉普捷夫海春季冰藻和浮游植物群落结构及生物量分析.
极地研究,2005,17(1):1-10
|
被引
8
次
|
|
|
|
|
3.
孙军. 2001/2002年夏季南极普里兹湾及其邻近海域的浮游植物.
海洋与湖沼,2003,34(5):519-532
|
被引
48
次
|
|
|
|
|
4.
朱根海. 2000年夏季南极普里兹湾及邻近海域浮游植物研究.
海洋学报,2006,28(1):118-126
|
被引
11
次
|
|
|
|
|
5.
Zhu G H. Phytoplankton in Prydz Bay and Its Adjacent Sea Area of Antarctica During the Austral Summer (1998/1999).
Acta Botanica Sinica,2003,45(4):390-398
|
被引
6
次
|
|
|
|
|
6.
朱根海. 全球气候变化对南极淡水藻类的影响.
中国环境科学,2010,30(3):400-404
|
被引
9
次
|
|
|
|
|
7.
华汝成.
单细胞藻类的培养与利用,1980
|
被引
17
次
|
|
|
|
|
8.
胡鸿钧.
中国淡水藻类--系统、分类及生态,2006
|
被引
84
次
|
|
|
|
|
9.
Nubel U. PCR primers to amplify 16S rRNA genes from cyanobacteria.
Applied and Environmental Microbiology,1997,63(8):3327-3332
|
被引
26
次
|
|
|
|
|
10.
Viprey M. Wide genetic diversity of picoplanktonic green algae (Chloroplastida) in the Mediterranean Sea uncovered by a phylum-biased PCR approach.
Environmental Microbiology,2008,10(7):1804-1822
|
被引
3
次
|
|
|
|
|
11.
Altschul S F. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.
Nucleic Acids Research,1997,25(17):3389-3402
|
被引
1186
次
|
|
|
|
|
12.
Cole J R. The ribosomal database project (RDP-II): introducing myRDP space and quality controlled public data.
Nucleic Acids Research,2006,35(Suppl. 1):169-172
|
被引
3
次
|
|
|
|
|
13.
Hall T A. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT.
Nucleic Acids Symposium Series,1999,41(41):95-98
|
被引
776
次
|
|
|
|
|
14.
Thompson J D. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools.
Nucleic Acids Research,1997,25(24):4876-4882
|
被引
2393
次
|
|
|
|
|
15.
Tamura K. MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4. 0.
Molecular Biology and Evolution,2007,24(8):1596-1599
|
被引
2001
次
|
|
|
|
|
16.
Huber T. Bellerophon: a program to detect chimeric sequences in multiple sequence alignments.
Bioinformatics,2004,20(14):2317-2319
|
被引
39
次
|
|
|
|
|
17.
毕列爵.
中国淡水藻志.(第八卷),2004
|
被引
1
次
|
|
|
|
|
18.
福笛B.
藻类学,1980
|
被引
1
次
|
|
|
|
|
19.
Chaudhary A. Bacterial and archaeal phylogenetic diversity of a cold sulfur-rich spring on the Shoreline of Lake Erie, Michigan.
Applied and Environmental Microbiology,2009,75(15):5025-5036
|
被引
5
次
|
|
|
|
|
20.
Novis P M. Two genetic and ecological groups of Nostoc commune in Victoria Land, Antarctica, revealed by AFLP analysis.
Antarctic Science,2006,18(4):573-581
|
被引
1
次
|
|
|
|
|
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