文摘
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目的 探讨天冬提取物作为流感疫苗佐剂的免疫增强作用,并应用二次回归正交旋转组合方法优化天冬佐剂与疫苗剂量。方法 将不同剂量的天冬提取物与H3N2流感灭活疫苗共同肌肉免疫小鼠,以相应剂量灭活疫苗的单独免疫组、灭活疫苗与氢氧化铝共同免疫组、生理盐水免疫组作为对照组,间隔3周,免疫2次,取尾血收集血清,测定血凝抑制(HI)抗体效价。并且应用二次回归正交旋转组合方法,以天冬提取物及流感疫苗血凝素(HA)含量作为2个关键因素设计分组,将HI效价作为响应值,使用SAS9.1软件进行数据分析,对天冬提取物及疫苗剂量组合进行研究,并得出最佳响应值对应的二者剂量。结果 天冬提取物能显著增强血清HI抗体效价,并且高于氢氧化铝佐剂。当流感疫苗HA含量为4.345μg、天冬提取物含量为0.1 mg时能够获得最高响应值(HI效价为5 092)。结论 天冬提取物对流感灭活疫苗具有佐剂效果,并有望成为流感灭活疫苗的新型免疫佐剂。 |
其他语种文摘
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Objective To explore the adjuvant effect of Radix Asparagi extracts on inactivated influenza virus vaccine and to optimize the dose of them by quadratic orthogonal rotation combination design.Methods Different dosages of Radix Asparagi extracts mixed with different dosages of inactivated influenza virus H3N2 vaccine were injected intramuscularly into mice.And mice immunized respectively with inactivated vaccine alone, aluminium-adjuvanted inactivated vaccine and physiological saline which were control groups.Each group of mice was immunized twice at 3 week intervals.Two weeks after the boost, blood samples were collected for measurement of serum hemagglutination inhibition(HI) titers.And further study on the dose of Radix Asparagi extracts and inactivated vaccine HA was evaluated by quadratic orthogonal rotation combination design with HI titers as response value.The data was analyzed by SAS9.1 software.In addition, the dose of Radix Asparagi extracts and inactivated vaccine HA with the optimal response value were defined.Result Radix Asparagi extracts significantly enhanced the HI titers, which was higher than those of aluminium.And when the dosage of Radix Asparagi extracts and inactivated vaccine HA respectively were 0.1mg and 4.345 μg, the optimal response value(HI titer) obtained was 5 092.Conclusion Radix Asparagi extracts can be used as a potential adjuvant of inactivated influenza virus vaccine. |
来源
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中国中医药信息杂志
,2011,18(7):63-65 【扩展库】
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关键词
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天冬提取物
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流感疫苗
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佐剂
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二次回归正交旋转组合设计
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地址
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1.
中国医学科学院北京协和医学院医学生物学研究所, 云南, 昆明, 650118
2.
中国科学院昆明植物研究所, 植物化学与西部植物资源持续利用国家重点实验室, 云南, 昆明, 650204
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语种
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中文 |
文献类型
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研究性论文 |
ISSN
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1005-5304 |
学科
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中国医学 |
基金
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国家高技术研究发展计划
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云南省联合支持国家资助项目
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文献收藏号
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CSCD:4387569
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