银鲫dmrt2b基因的分子特征及功能分析
MOLECULAR CHARACTERIZATION AND FUNCTIONAL ANALYSIS OF GIBEL CARP DMRT2b
查看参考文献19篇
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文摘
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实验克隆了银鲫dmrt2b基因的全长cDNA序列,并对其表达图式和在胚胎发育过程中的功能做了初步研究。银鲫dmrt2b和斑马鱼dmrt2b有相似的基因组结构。在胚胎发育过程中,银鲫dmrt2b主要在体节中表达。在成体中主要分布于肌肉中。注射银鲫的dmrt2b可以挽救斑马鱼dmrt2b敲降胚的表型。上述结果表明银鲫dmrt2b基因同斑马鱼dmrt2b基因有相同的功能。 |
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其他语种文摘
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Gibel carp(Carassius auratus gibelio) is a good model animal for evolutionary developmental biology.Our previous studies have already identified a novel fish-specific duplicated gene dmrt2b from gibel carp.Then we found that, in zebrafish, duplicated genes dmrt2b and dmrt2a played common overlapping functions in the establishment of left-right asymmetry of the body plan, and dmrt2b contributed to a divergent function in somitogenesis through Hedge-hog pathway, but dmrt2a did not contribute to Hedgehog pathway.In this study, we cloned the full-length cDNA of gibel carp dmrt2b.The entire dmrt2b cDNA sequence was 1938 bp long and the open read frame was 1014 long, which en-coded a protein with 337 amino acids.Gibel carp dmrt2b had a genomic organization similar to zebrafish dmrt2b, which had same numbers and positions of exons and introns.Therefore, gibel carp dmrt2b and zebrafish dmrt2b were orthologs.Then we analyzed expression pattern and functions involved of gibel carp dmrt2b during embryogenesis.By whole mount in situ hybridization, we found gibel carp dmrt2b mainly localized in the somites during embryogenesis.Then it mainly expressed in muscle of adult fish.This expression pattern was also similar to that of zebrafish dmrt2b.Microin-jection of gibel carp dmrt2b mRNA into zebrafish dmrt2b morphants could rescue the phenotype.Above results sug-gested that gibel carp dmrt2b might have the same function with zebrafish dmrt2b during the embryogenesis. |
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来源
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水生生物学报
,2011,35(3):379-383 【核心库】
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关键词
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银鲫
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dmrt2b
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表达模式
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基因结构
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胚胎发育
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地址
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中国科学院水生生物研究所, 淡水生态与生物技术国家重点实验室, 武汉, 430072
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语种
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中文 |
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文献类型
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研究性论文 |
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ISSN
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1000-3207 |
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学科
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分子生物学 |
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基金
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国家现代农业产业技术体系建设专项资金项目
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国家自然科学基金
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中国科学院水生生物研究所淡水生态与生物技术国家重点实验室基金
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中国科学院水生生物研究所领域项目资助
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文献收藏号
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CSCD:4384276
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