棕色棉F3'H-羟化酶基因的克隆与生物信息学分析
Cloning and Bioinformatics Analysis of Flavonoid 3'-Hydroxylase Gene GhF3'Hin Brown Cotton (Gossypium hirsutum L.) Fibers
查看参考文献13篇
|
文摘
|
根据葡萄的类黄酮3'-羟化酶（F3'H）基因全长cDNA序列Blast所得棉花的EST序列设计引物，以开花后16 d （DPA16）的新彩棉5号（XC-5）纤维为材料，利用RACE和RT-PCR技术分离得到了2个类黄酮3'-羟化酶基因cDNA序列，此2个序列编码区完全相同，仅在3'UTR区存在片段长短的差异，推测可能是基因转录后加工方式不同所造成。克隆所获得的棉花F3'H基因编码区全长1 533 bp，编码510个氨基酸，氨基酸序列分析预测表明，该基因所编码蛋白含有一个跨膜结构域，是一种分泌蛋白，定位于内质网上，并含有一段与细胞色素P450功能区相匹配的保守功能域；序列比对结果表明，棉花F3'H基因与其他多个物种的F3'H基因在氨基酸序列上有较高的同源性；聚类分析结果表明，棉花F3'H蛋白与双子叶植物大豆的F3'H亲缘关系较为接近，而与单子叶植物高粱等作物则较远。 |
|
其他语种文摘
|
Two full-length cDNA sequences encoding flavonoid 3'-hydroxylase gene (F3'H) were cloned from brown-colored cotton (Gossypium hirsutum L.) cultivar "XC-5" fibers of 16 days post anthesis (DPA) by rapid amplification of cDNA ends (RACE) and RT-PCR technique using specific primers based on the EST sequences from the blast results with the full-length cDNA of F3'H in Vitis vinifera and were named as GhF3'H-1 and GhF3'H-2 (GenBank accession: HM598123, HM598124). The two cDNA sequences contain an identical opening reading frame (ORF) of 1 533 bp in length, encoding a protein of 510 amino acids. There are few differences between the two sequences except for the sequence length of the 3'untranslational region (UTR), which might result from the difference of post-transcriptional processing. The bioinformatics analyses showed that, the GhF3'H protein, holding with a membrane spanning domain, are located on endoplasmic reticulum and belongs to secretory proteins; in addition, it contains a conservative functional domain which matches the cytochrome P450 binding domain; the GhF3'H gene shows the highly homogeneity at amino acid level in comparison to the F3'H genes of several other higher plant species; the phylogenetic analysis of F3'H genes from different species reveals that the GhF3'H has closer relationship with the F3'H from Glycine max than from other plant species. |
|
来源
|
生物技术通报
,2011(11):199-206 【扩展库】
|
|
关键词
|
类黄酮3'-羟化酶
;
棕色棉
;
RACE
;
序列分析
|
|
地址
|
1.
中国科学院新疆理化技术研究所, 中国科学院新疆植物资源化学重点实验室, 乌鲁木齐, 830011
2.
中国彩棉（集团）股份有限公司, 乌鲁木齐, 830016
|
|
语种
|
中文 |
|
文献类型
|
研究性论文 |
|
ISSN
|
1002-5464 |
|
学科
|
农作物 |
|
基金
|
中国科学院新疆理化技术研究所博士启动资金项目
;
中国科学院西部之光人才培养计划
;
国家转基因生物新品种培育重大专项
|
|
文献收藏号
|
CSCD:4369830
|
参考文献 共
13
共1页
|
|
1.
杜雄明. 有色棉的研究利用现状及展望.
中国农学通报,1997,13(3):30-32
|
被引
22
次
|
|
|
|
|
2.
华水金. 棕色棉纤维发育过程中碳水化合物和色素的变化特征.
棉化学报,2008,20(3):239-241
|
被引
7
次
|
|
|
|
|
3.
于伯龄. 绿色天然彩色棉金属盐固色试验.
针织工业,2002(1):35-37
|
被引
5
次
|
|
|
|
|
4.
Schuler M A. Functional genomics of P450s.
Annu Rev Plant Biol,2003,54:629-667
|
被引
52
次
|
|
|
|
|
5.
Werck-Reichhart D. Cytochromes P450: a success story.
Genome Biol,2000,1(6):30031-30039
|
被引
60
次
|
|
|
|
|
6.
Koes R E. The flavonoid biosynthetic pathway in plants: function and evolution.
BioEssays,1994,16(2):123-132
|
被引
75
次
|
|
|
|
|
7.
Wan C Y. A modified hot borate method significantly enhances the yield of high-quality RNA from cotton (Gossypium hirsutum L.).
Anal Biochem,1994,223(1):7-12
|
被引
137
次
|
|
|
|
|
8.
宋洋. 制备棉花幼蕾高质量总RNA的方法比较.
棉花学报,2008,20(3):231-223
|
被引
8
次
|
|
|
|
|
9.
Toguri T. Cloning of eggplant hypocotyl cDNAs encoding cytochromes P450 belonging to a novel family (CYP77).
Febs Lett,1994,338(3):290-294
|
被引
6
次
|
|
|
|
|
10.
Holton T A. Genetics and biochemistry of anthocyanin biosynthesis.
Plant Cell,1995,7(7):1071-1083
|
被引
271
次
|
|
|
|
|
11.
Schuler M A. Plant cytochrome P450 monooxygenases.
Crit Rev Plant Sci,1996,15(3):235-284
|
被引
30
次
|
|
|
|
|
12.
Chapple C. Molecular-genetic analysis of plant cytochrome P450-dependent monooxygenases.
Annu Rev Plant Biol,1998,49:311-343
|
被引
64
次
|
|
|
|
|
13.
Bruglier A F. Isolation and characterization of a favonoid 3'-hydroxylase cDNA clone corresponding to the Ht1 locus of Petunia hybrida.
Plant J,1999,19(4):441-451
|
被引
2
次
|
|
|
|
|
了解气象卫星更多信息，请访问