嗜热四膜虫金属硫蛋白基因MMT2和MTT4的表达规律比较与进化模式分析
Function comparison and evolution analysis of metallothionein gene MTT2 and MTT4 in Tetrahymena thermophila
查看参考文献41篇
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文摘
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多个金属硫蛋白基因异构型已在四膜虫中被鉴定，这些异构型可分为7a和7b两个亚家族。该文利用实时荧光定量PCR技术检测了嗜热四膜虫金属硫蛋白基因MTT2和MTT4在Hg、Cu、Cd、Zn、H_2O_2暴露下的表达水平，结果显示两者表达规律相似，均为：Cu暴露下上调最高(>200倍)，Hg次之，Cd、Zn上调倍数不大，H_2O_2有下调趋势。此表达规律明显有别于7a亚家族，具有7b亚家族的表达特征。同种诱导物暴露下MTT4的上调表达幅度比MTT2高出数倍，结合生物信息学分析结果，推测可能与MTT2和MTT4上游调控元件(如AP-1、MRE等)的数量差异有关。基于MTT2和MTT4在结构和功能上的高度相似性，推测两者可能是经近期基因复制事件产生，并遵循基因剂量模型进化而来。 |
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其他语种文摘
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Tetrahymena has a high genetic polymorphism of metallothionein proteins.These protein isoforms can be divided into subfamilies 7a and 7b.We used real-time quantitative PCR to test the expression levels of Tetrahymena thermophila metallothionein genes MTT2 and MTT4 after exposure to different inducers, including Hg, Cu, Cd, Zn and H_2O_2.Both genes were most efficiently induced by Cu and secondly by Hg.Their expression was slightly up-regulated after exposure to Cd and Zn, but down-regulated by H_2O_2.The expression pattern differed from those reported in the 7a subfamily, but was consistent with 7b subfamily members.However, the induced expression level of MTT4 was much higher than that of MTT2, which might be due to differences in their upstream regulatory elements(AP-1, MRE).The high similarities in gene structures and functions indicate that MTT2 and MTT4 were generated by recent gene duplication following the dosage balance model. |
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来源
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动物学研究
,2011,32(5):476-484 【核心库】
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关键词
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嗜热四膜虫
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金属硫蛋白基因
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实时荧光定量PCR
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表达分析
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基因复制
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地址
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中国科学院水生生物研究所, 中国科学院水生生物多样性与保护重点实验室, 湖北, 武汉, 430072
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语种
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中文 |
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文献类型
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研究性论文 |
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ISSN
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0254-5853 |
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学科
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生物化学;动物学 |
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基金
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武汉市学科带头人项目
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文献收藏号
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CSCD:4347382
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参考文献 共
41
共3页
|
|
1.
Amaro F. Two new members of the Tetrahymena multi-stress-inducible metallothionein family: Characterization and expression analysis of T. rostrata Cd/Cu metallothionein genes.
Gene,2008,423(1):85-91
|
被引
3
次
|
|
|
|
|
2.
Aury J M. Global trends of whole-genome duplications revealed by the ciliate Paramecium tetraurelia.
Nature,2006,444(7116):171-178
|
被引
15
次
|
|
|
|
|
3.
Binz P A. Metallothionein: Molecular Evolution and Classification.
Metallothionein IV,1999:7-13
|
被引
4
次
|
|
|
|
|
4.
Boldrin F. MTT2, a copper-inducible metallothionein gene from Tetrahymena thermophila.
Comp Biochem Phys C,2008,147(2):232-240
|
被引
1
次
|
|
|
|
|
5.
Boldrin F. Cloning and sequencing of four new metallothionein genes from Tetrahymena thermophila and T. pigmentosa: Evolutionary relationships in Tetrahymena MT family.
Protist,2003,154(3/4):431-442
|
被引
6
次
|
|
|
|
|
6.
Colangelo D. Protective effect of metallothioneins against oxidative stress evaluated on wildtype and MT-null cell lines by means of flow cytometry.
Biometals,2004,17(4):365-370
|
被引
3
次
|
|
|
|
|
7.
Coyle P. Ethanol-mediated fetal dysmorphology and its relationship to the ontogeny of maternal liver metallothionein.
Alcohol Clin Exp Res,2009,33(6):1051-1058
|
被引
1
次
|
|
|
|
|
8.
Coyle P. Metallothionein: The multipurpose protein.
Cell Mol Life Sci,2002,59(4):627-647
|
被引
70
次
|
|
|
|
|
9.
Cupples C G. Isolation and characterization of the actin gene from Tetrahymena thermophila.
Proc Natl Acad Sci USA,1986,83(14):5160-5164
|
被引
1
次
|
|
|
|
|
10.
Dabrio M. Recent developments in quantification methods for metallothionein.
J Inorg Biochem,2002,88(2):123-134
|
被引
19
次
|
|
|
|
|
11.
Diaz S. Tetrahymena metallothioneins fall into two discrete subfamilies.
PLoS One,2007,2(3):e291
|
被引
6
次
|
|
|
|
|
12.
Dondero F. Biochemical characterization and quantitative gene expression analysis of the multi-stress inducible metallothionein from Tetrahymena thermophila.
Protist,2004,155(2):157-168
|
被引
3
次
|
|
|
|
|
13.
Feng L. Differentially expressedgenes of Tetrahymena thermophila in response to Tributyltin(TBT)identified by suppression subtractive hybridization and real time quantitative PCR.
Aquat Toxic,2007,81(1):99-105
|
被引
2
次
|
|
|
|
|
14.
Fu C J. Cloning and characterization of a new multi-stress inducible metallothionein gene in Tetrahymena pyriformis.
Protist,2006,157(2):193-203
|
被引
1
次
|
|
|
|
|
15.
Guo L N. Cloning, characterization, and gene expression analysis of a novel cadmium metallothionein gene in Tetrahymena pigmentosa.
Gene,2008,423(1):29-35
|
被引
1
次
|
|
|
|
|
16.
Haq F. Signaling events for metallothionein induction.
Mutat Res,2003,533(1/2):211-226
|
被引
14
次
|
|
|
|
|
17.
Imbert J. Regulation of metallothionein gene transcription by metals.
Adv Inorg Biochem,1990,8:139-164
|
被引
1
次
|
|
|
|
|
18.
Innan H. The Evolution of gene duplications: Classifying and distinguishing between models.
Nat Rev Genet,2010,11(2):97-108
|
被引
23
次
|
|
|
|
|
19.
Kagi J H R. Evolution, Structure and Chemical Activity of Class-I Metallothioneins-an Overview.
Metallothionein III-Biological Roles and Medical Implications,1993:29-55
|
被引
1
次
|
|
|
|
|
20.
Kramer K K. Induction of metallothionein mrna and protein in murine astrocyte cultures.
Toxicol Appl Pharm,1996,136(1):94-100
|
被引
2
次
|
|
|
|
|
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