茶叶中有效成分提取工艺研究
Study on Extraction Process of Available Components of Tea
查看参考文献11篇
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文摘
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目的：优选茶叶中主要有效成分的最佳提取工艺。方法：分别以儿茶素(CT)包括其单体表没食子儿茶素没食子酸酯(EGCG)和咖啡因(CF)的含量和浸膏得率、清除DPPH·的活性为评价指标，选择乙醇浓度(A)、料液比(B)和提取时间(C)为考察因素，采用L_9(34)正交试验优选茶叶有效成分的最佳提取工艺。结果：根据不同的提取目的选择不同的评价指标加权分析会得到不同的提取工艺条件。若以提取CT为目的，则选浸膏得率、 EGCG和CT的含量、清除DPPH·的活性为评价指标得最佳提取工艺为：12倍量75%乙醇，提取3次，每次30 min；若以提取CF为目的，则选浸膏得率和CF的含量为评价指标得最佳提取工艺为：12倍量60%乙醇，提取3次，每次 30 min；若以综合提取为目的，则选浸膏得率、CT和CF的含量、清除DPPH·的活性为评价指标得最佳提取工艺为：8倍量60%乙醇，提取3次，每次30 min。结论：根据不同的提取目的优选工艺条件可为工业化生产提供参考。 |
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其他语种文摘
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Objective: To investigate the optimum ethanol extraction process conditions for the available components in the tea-Catechines(CT) including Epigallo catechin gallate(EGCG) and Caffeine(CF). Methods: The content of EGCG, CT and CF, extraction rate, DPPH·Free radical scavenging capacity were chosen as the assessment indexes. With the alcohol ratio(A), solid-liquid ratio(B) and reflux time(C) as investigation factors, the optimum ethanol extraction process of the available components from tea was determined by L_9 (3~4) orthogonal experimental design. Results: It would obtain different extraction conditions to analyze the assessment indexes depending on the different extraction purposes. For the purpose of CT, the contents of EGCG and CT, extraction rate and DPPH·Free radical scavenging capacity were chosen as the assessment indexes, the optimum extraction conditions were selected as follows: the ratio of raw material to 75% alcohol was 1:12, the reflux time was 30 minutes and extraction times were three; For the purpose of CF, the content of CF and extraction rate were chosen as the assessment indexes, the optimum extraction conditions were selected as follows: the ratio of raw material to 60% alcohol was 1:12, the reflux time was 30 minutes and extraction times were three; For the purpose of integrated extraction, the contents of CT and CF, extraction rate and DPPH·Free radical scavenging capacity were chosen as the assessment indexes, the optimum extraction conditions were selected as follows: the ratio of raw material to 60% alcohol was 1:8, the reflux time was 30 minutes and extraction times were three. Conclusion: The optimum extraction process in order to attain different purposes can give a reference to the research of a new medicine and industry production. |
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来源
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中药材
,2011,34(9):1438-1441 【核心库】
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关键词
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茶叶
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提取工艺
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正交试验
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地址
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1.
甘肃中医学院药学系, 甘肃省高校中(藏)药化学与质量研究省级重点实验室;;西北特色植物资源化学重点实验室;;甘肃省天然药物重点实验室, 甘肃, 兰州, 730000
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中国科学院兰州化学物理研究所, 中国科学院西北特色植物资源化学重点实验室;;甘肃省天然药物重点实验室, 甘肃, 兰州, 730000
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甘肃中医学院药学系, 甘肃省高校中(藏)药化学与质量研究省级重点实验室, 甘肃, 兰州, 730000
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语种
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中文 |
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ISSN
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1001-4454 |
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学科
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中国医学 |
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基金
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国家自然科学基金面上项目
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文献收藏号
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CSCD:4338663
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