濒危植物华木莲核基因组微卫星引物开发研究
The Development of Nuclear Microsatellite Markers for an Endangered Plant, Sinomanglietia glauca (Magnoliaceae)
查看参考文献30篇
文摘
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华木莲(Sinomanglietia glauca)是在江西宜春和湖南永顺发现的一种珍稀植物。为进一步探讨华木莲群体遗传学特征,本研究采用FIASCO法(fast isolation by AFLP sequences containing repeats)开发了七对华木莲微卫星引物,选取华木莲4个野生居群16个样品对每个位点进行多态性初步检测。结果发现,七个微卫星位点的等位基因数目(N_A)为2~7个,观测杂合度(H_O)0.079 ~0.989,期望杂合度(H_E)0.187~0.600。另外,使用九个华木莲近缘种对七对引物进行通用性检测,发现这些引物在醉香含笑中通用性最低(28.5%),在观光木中通用性最高(85.7%)。本文开发的多态性微卫星标记将用于华木莲的繁育系统、群体遗传学等研究,同时也将用于木兰科其它近缘种的群体遗传学研究。 |
其他语种文摘
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Sinomanglietia glauca is an endangered species found in Yichun of Jiangxi Province and Yongshun of Hunan Province. To further explore its population genetic characteristics, we developed seven microsatellite markers from S.glauca using a FIASCO (fast isolation by AFLP sequences containing repeats) method. The number of alleles (N_A) ranged from two to seven in 16 samples of four wild populations. The ranges of observed (H_O) and expected (H_E) heterozygosities were 0.079-0.989 and 0.187-0.600, respectively. The transferability of the seven microsatellite loci was tested upon nine related species of S.glauca within Magnoliaceae. Among which, two (28.5%, Michelia macclurei) to six (85.7%, Tsoongiodendron odorum) loci were successfully amplified. These polymorphic microsatellite markers could be used to decipher the genetic structure, breeding system and population history of S.glauca as well as other relatives within Magnoliaceae. |
来源
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植物分类与资源学报
,2011,33(5):535-539 【核心库】
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关键词
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华木莲
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微卫星
;
分子标记
;
通用性
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地址
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1.
江西农业大学亚热带生物多样性实验室, 江西, 南昌, 330045
2.
中国科学院昆明植物研究所, 云南, 昆明, 650201
3.
江西农业大学亚热带生物多样性实验室, 系统与进化植物学国家重点实验室, 江西, 南昌, 330045
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语种
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中文 |
文献类型
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研究性论文 |
ISSN
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2095-0845 |
学科
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分子生物学 |
基金
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江西省自然科学基金
;
系统与进化植物学国家重点实验室开放课题
;
国家自然科学基金
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文献收藏号
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CSCD:4319842
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