应用BIGIS-4测序系统完成Glaciecola mesophila sp.nov.的全基因组测序和组装
查看参考文献25篇
文摘
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BIGIS-4是中国新一代基于焦磷酸测序技术的测序仪.本实验利用BIGIS-4完成了对Glacielola mesophila sp.nov.(Gmn)的全基因组测序.Gmn是一株从海洋无脊椎动物体内分离到的革兰氏阴性菌.BIGIS-4测序得到152043个高质量的测序片段,平均读长406bp.测序片段由BIGIS-4系统后处理模块组装.除单核苷酸同聚体引起的测序错误外,测序结果中没有检测到其他低质量错误.Gmn基因组全长5144318bp,共注释得到4303个基因,其中有大量的代谢基因,与菌种在海洋表面非脊椎动物的生长环境相关.Gmn的冷适应和信号转导相关基因为其对海洋低温环境的适应提供了依据. |
来源
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中国科学. 生命科学
,2011,41(7):544-549 【核心库】
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关键词
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Glacielola mesophila sp.nov.
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BIGIS-4测序系统
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海洋环境的适应
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地址
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1.
中国科学院北京基因组研究所, 中国科学院基因组科学及信息重点实验室, 北京, 100029
2.
中国科学院半导体研究所, 集成光电子学国家重点实验室, 北京, 100083
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中国极地研究中心, 国家海洋局极地科学重点实验室, 上海, 200136
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语种
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中文 |
文献类型
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研究性论文 |
ISSN
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1674-7232 |
学科
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分子生物学 |
基金
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中国科学院科研装备研制项目
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国家自然科学基金
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中国科学院北京基因组研究所自主立项经费资助
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文献收藏号
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CSCD:4268495
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