小叶龙竹的快速繁殖与离体保存
Rapid Propagation and Reservation in vitro of Dendrocalamus barbatus Hsueh et D.Z.Li
查看参考文献13篇
文摘
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利用小叶龙竹种子培养的试管苗对其快速繁殖体系的建立与离体保存进行了研究,结果表明:根、茎、叶和小芽各外植体中,小芽切段可以较好的诱导芽的增殖且基本培养基MS附加3mg/LBA和0.1mg/LNAA为芽增殖最适培养基,增殖率可达1∶4.4。培养基1/2MS附加1mg/LNAA和1mg/LIBA为最佳生根培养基,15d丛芽生根率为93%,27d丛芽生根率则达到了100%。MS培养基附加10mg/LCCC培养基为最佳离体保存培养基,保存时间可达120d以上,转接到MS外加3mg/LBA和0.1mg/LNAA的恢复培养基上,培养50 d,可以100%恢复生长。据调查:小叶龙竹的快速繁殖与离体保存研究国内外尚属首次报道。 |
其他语种文摘
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The test-tube plantlets cultured from the seeds of Dendrocalamus barbatus Hsueh et D.Z.Li were utilized to investigate the system of rapid propagation and reservation in vitro,the results revealed that the shoot of seedlings was a better one of the explants that could induce shoots propagation,for which the MS medium supplemented with 3 mg/L BA and 0.1 mg/L NAA was the best one and the ratio of propagation can be 1 to 4.4.While the 1/2 MS medium supplemented with 1 mg/L NAA and 1 mg/L IBA was the best root medium in which the rooting ratio can be 93% in 15 days and 100% in 27days.The plantlets could be sustained more than 120 days in the best reservation MS medium in vitro supplemented with 10 mg/L CCC,which also could survive and grow in 50 days in the MS medium supplemented with 3 mg/L BA and 0.1 mg/L NAA.As reported,this research is the latest fruits in the world. |
来源
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云南农业大学学报
,2011,26(3):359-363 【扩展库】
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关键词
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小叶龙竹
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快速繁殖
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离体保存
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地址
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1.
云南农业大学农学与生物技术学院, 云南, 昆明, 650201
2.
中国科学院昆明植物研究所, 云南, 昆明, 650204
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语种
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中文 |
文献类型
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研究性论文 |
ISSN
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1004-390X |
学科
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林业 |
基金
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中国西南野生生物种质资源库
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文献收藏号
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CSCD:4203386
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