UV-Vis光谱差减法测定油橄榄叶中橄榄苦苷含量初探
Study on the Content Determination of Oleuropein in Olea europaea L. Leaves by Substraction of UV - Visible Spectroscopy
查看参考文献19篇
文摘
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建立了紫外-可见(UV-Vis)分光光谱测定油橄榄叶中橄榄苦苷含量的方法. 以芦丁和橄榄苦苷为对照品,分别采用NaNO2-Al(NO3)3-NaOH络合体系和AlCl3络合体系进行显色. 以NaNO2-Al(NO3)3-NaOH体系显色后于510 nm处可测得油橄榄叶中总黄酮和橄榄苦苷的吸光度之和;而以AlCl3体系显色后于 415 nm处仅测得总黄酮的吸光度,橄榄苦苷无吸收. 利用橄榄苦苷在两种显色体系中的吸收特性差别,通过差减法换算后可间接测定样品中橄榄苦苷的含量. 以UV-Vis差减法测得油橄榄叶及其纯化浸膏中橄榄苦苷的平均含量分别为11.6%和63.4%, 3次测定的相对标准偏差(RSD)分别为1.5%和1.2%,其结果与高效液相色谱法(HPLC)测定结果的相对误差小于4.0%. 该方法可替代HPLC用于油橄榄叶中橄榄苦苷的含量测定,为油橄榄叶及其制品的质量控制提供了一种快速、简便、成本低廉的分析方法 |
其他语种文摘
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The content of oleuropein was usually determined by high performance liquid chromatography(HPLC) or gas chromatography(GC) method in previous studies. In this paper, a method for the determination of oleuropein in Olea europaea L. leaves was developed using a substraction of UV - Visible spectroscopy. With the standard rutin and oleuropein, two coloration systems including NaNO_2- Al(NO_3)_3 - NaOH and A1Cl_3 were both used. The total absorbance of total flavonoids and oleuropein was obtained at a wavelength of 510 nm in the former coloration system. In the latter coloration system, the absorbance of total flavonoid was obtained at a wavelength of 415 nm, but oleuropein was not detected at 415 nm in this system. According to the above absorbance properties, the content of oleuropein could be indirectly learned by substraction of the absorbances obtained in the two coloration systems. By using the developed UV - Vis method, the average contents of oleuropein in the olive leaves and purified extractums were 11. 6% and 63. 4% with RSDs (n = 3) of 1. 5% and 1. 2% , respectively. Compared with the content determined by HPLC method, the relative error of this method was less than 4% . The result showed that this method could be used to replace the HPLC method for the determination of the content of oleuropein in the olive leaves or its products as the former was more simple, faster and lower cost than the latter. It can be looked as a reference method for the indirect determination of the active components in herbal materials or natural products |
来源
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分析测试学报
,2011,30(3):242-247 【核心库】
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关键词
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油橄榄叶
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橄榄苦苷
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紫外-可见吸收光谱
;
差减法
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地址
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中国科学院兰州化学物理研究所, 中科院西北特色植物资源化学重点实验室;;甘肃省天然药物重点实验室, 甘肃, 兰州, 730000
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语种
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中文 |
ISSN
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1004-4957 |
学科
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化学;农作物 |
基金
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中国科学院科技支甘项目
;
中国科学院“百人计划”项目
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文献收藏号
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CSCD:4176299
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参考文献 共
19
共1页
|
1.
MANUEL B. Phenolic compounds in spanish olive oils.
J Agric Food Chem,1999,47(9):3535-3540
|
被引
2
次
|
|
|
|
2.
BENAVENTE G O. Antioxidant activity of phenolics extracted from Olea europaea L. leaves.
Food Chem,2000,68(4):457-462
|
被引
2
次
|
|
|
|
3.
PEREZ B M. Isolation and identification of radical scavengers in olive tree(Olea europaea) wood.
J Chromatogr: A,2006,1112(1/2):311-318
|
被引
4
次
|
|
|
|
4.
SUDJANA A N. Antimicrobial activity of commercial Olea europaea(olive) leaf extract.
Int J Antimicrob Agents,2009,33(5):461-463
|
被引
5
次
|
|
|
|
5.
SCHEFFLER A. Olea europaea leaf extract exerts L-type Ca2+ channel antagonistic effects.
J Ethnopharmacol,2008,120(2):233-240
|
被引
1
次
|
|
|
|
6.
RAFFAELLA B. Olea europaea L. leaf extract and derivatives: antioxidant properties.
J Agric Food Chem,2002,50(17):4934-4940
|
被引
2
次
|
|
|
|
7.
JAPON L R. Multivariate optimisation of the microwave-assisted extraction of oleuropein and related biophenols from olive leaves.
Anal Bioanal Chem,2006,385(4):753-759
|
被引
1
次
|
|
|
|
8.
ALTOK E. Isolation of polyphenols from the extracts of olive leaves (Olea europaea L.) by adsorption on silk fibroin.
Sep Purif Technol,2008,62(2):342-348
|
被引
1
次
|
|
|
|
9.
BRIANTE R. Bioactive derivatives from oleuropein by a biotransformation on Olea europaea leaf extracts.
J Biotechnol,2002,93(2):109-119
|
被引
5
次
|
|
|
|
10.
彭焕超. RP-HPLC测定不同来源油橄榄叶中的橄榄苦苷.
华西药学杂志,2008,23(2):199-200
|
被引
1
次
|
|
|
|
11.
SAVOURNIN C. Rapid high-performance liquid chromatography analysis for the quantitative determination of oleuropein in Olea europaea leaves.
J Agric Food Chem,2001,49(2):618-621
|
被引
8
次
|
|
|
|
12.
ORTEGA G F. Phenylalanine ammonialyase and phenolic compounds in leaves and fruits of Olea europaea L. cv. Picual during ripening.
J Sci Food Agric,2009,89(3):398-406
|
被引
1
次
|
|
|
|
13.
王成章. HPLC研究油橄榄叶中橄榄苦苷的含量变化规律.
林产化学与工业,2008,28(6):39-43
|
被引
11
次
|
|
|
|
14.
RANALLI A. Factors affecting the contents of iridoid oleuropein in olive leaves(Olea europaea L.).
J Agric Food Chem,2006,54(2):434-440
|
被引
4
次
|
|
|
|
15.
郑媛媛. 油橄榄叶中总黄酮含量测定方法探讨.
光谱学与光谱分析,2011,31(2):547-550
|
被引
34
次
|
|
|
|
16.
钱浩泉. 中药液相色谱指纹图谱的方法学研究--银杏叶总黄酮指纹图谱个例剖析.
分析测试学报,2004,23(5):7-11
|
被引
12
次
|
|
|
|
17.
侯冬岩. 东北贯众中总黄酮的光谱分析.
分析测试学报,2004,23(3):110-112
|
被引
2
次
|
|
|
|
18.
高彩霞. 油橄榄叶中多酚和黄酮的含量分析.
生物质化学工程,2006,40(4):4-6
|
被引
9
次
|
|
|
|
19.
回瑞华. 三波长-光谱法测定沙棘果汁中黄酮的含量.
光谱学与光谱分析,2005,25(2):266-269
|
被引
11
次
|
|
|
|
|