高效液相色谱-质谱法分析啶菌唑在番茄灰霉病菌中的代谢行为
Metabolism Dynamics and Metabolite of SYP-Z048 in Botrytis Cinerea by High Performance Liquid Chromatography-Mass Spectrometry
查看参考文献15篇
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文摘
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采用高效液相色谱-质谱法(HPLC-MS)分析了杀菌剂啶菌唑在番茄灰霉病菌液体摇培体系中的代谢动态. 啶菌唑液相色谱定量检测法的线性回归方程为y=54.5x+11.2(r=0.9997),在2.07~72.34 mg/L范围内,方法回收率和精密度符合农药痕量分析要求. 代谢影响因子研究结果显示,培养24 h孢子初萌发阶段的灰霉病菌对药剂代谢能力显著优于后期菌丝生长阶段. 啶菌唑代谢量与接菌量呈正相关. 代谢动态研究表明,在0.05, 0.10和0.21 mg/L添加浓度下,啶菌唑与灰霉病菌经过44 h的共培养,分别降解为初始浓度的53.8%, 58·2%和 68.6%,代谢随药剂浓度降低而增快,而在不含菌的培养基中降解率低于0.6%(0.21 mg/L). 药剂处理灰霉病菌培养液经HPLC分离获得4个疑似代谢产物峰,其中化合物M2为分子量257的含氯代谢产物. 啶菌唑在灰霉病菌菌体中发生了代谢作用,代谢速度受菌龄、接菌量和药剂浓度的影响 |
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其他语种文摘
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High performance liquid chromatography-mass spectrometry was used to study the metabolism of a new fungicide 5-(4-chloro pheny1)-2, 3-dimethyl-3-(pyri-dine-3)-oxazoline (SYP-Z048) in Botrytis cinerea. The fungus was cultivated in liquid culture medium with SYP-Z048 and the concentration was determined by high performance liquid chromatography. The linear equation wasy=8466. 1x-46.3(r=0.9995) in the concentration range of 0.02-0.31 mg/L. Impact factors of metabolism were studied. The results show that SYP-Z048, at the concentration of 0.05, 0.10 and 0.21 mg/L, was reduced to 46.2%, 41.8% and 31.4% respectively after cultivated withB. cinereafor 44 h, but the degradation rate was less than 0.6% without fungi at 0.21 mg/L. The results indicated that SYP-Z048 was metabolized accelerated at lower dosage. The metabolic rate that affected by young mycelium after cultivated for 24 h was obviously higher than that of the mature one. There was a positive correlation between the quantity reduced after a definite period and the amount of B. cinereaput in medium. HPLC-MS was adopted to analyze the metabolic products of SYP-Z048. There were four possible metabolites peaks in chromatogram, of which the compound M2is chlorine with a molecule weight of 257. The study showed that SYP-Z048 metabolized inBotrytis cinereaand the metabolize rate was affected by the age and amount of mycelium |
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来源
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分析化学
,2011,39(3):317-322 【核心库】
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关键词
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啶菌唑
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番茄灰霉
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代谢
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液相色谱-串联质谱
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地址
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1.
中国农业大学农学与生物技术学院, 北京, 100193
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北京农产品质量检测与农田环境监测技术研究中心, 北京, 100097
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沈阳化工研究院农药生物测定中心, 沈阳, 110021
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语种
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中文 |
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文献类型
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研究性论文 |
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ISSN
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0253-3820 |
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学科
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化学工业 |
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基金
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国家“十一五”科技支撑计划项目
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国家教育部新世纪优秀人才支持计划
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中国农业大学-南京农业大学青年教师开放科研基金
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文献收藏号
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CSCD:4170734
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