人结肠癌裸鼠肝转移模型的建立
Establishment and Comparison of Colon Cancer Liver Metastasis Models in Nude Mice
查看参考文献8篇
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文摘
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目的建立人结肠癌裸鼠肝转移模型,用于肿瘤转移防治的实验研究.方法 30只裸鼠随机分为两组,分别用肝脏注射法和脾脏注射法建立结肠癌肝转移模型,术后待裸鼠濒临死亡或自然死亡时,观察其生存天数,解剖裸鼠,观察腹腔内转移情况和肝脏成瘤的情况.结果两组裸鼠的肝脏成瘤率均为100%,生存期无明显差异.肝脏注射组裸鼠均于注射部位出现单一的巨大瘤结节,脾脏注射组裸鼠肝脏表面及切面见多发散在的转移瘤结节.两组的肝脏瘤组织细胞形态学相似,符合低分化腺癌的特征.结论经脾注射法建立裸鼠结肠癌肝转移模型比较符合结肠癌体内肝转移过程的规律,是一种简便有效的,重复性好的方法 |
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其他语种文摘
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Objective To develop a reliable colon cancer liver metastasis model in nude mice. Methods Thirty nude mice were divided into two groups randomly, which were the liver injection group and spleen injection group, respectively containing 15 nude mice. Colon cancer liver metastasis models were made by liver injection and spleen injection, respectively. The average survival time was recorded. The metastasis of nude mice was detected pathologically. Results Both of the incidence rates of liver metastasis in the two groups were 100%. Survival time of the two groups showed no statistics significance. Anatomical study showed that there appeared a single giant tumor nodule appeared at every injection site in the liver injection group while multiple metastasis appeared nodules in livers in the spleen injection group. The pathological results indicated that the tumor cells pattern of liver metastasis was similar in the two groups and was in accordance with poorly differentiated colon adenocarcinoma. Conclusion Intrasplenic injection of colon cancer cells, which closely mimic the natural metastatic process,is a reliable method for producing colon cancer liver metastasis in nude mice and should be proved valuable in experimental study |
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来源
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肿瘤防治研究
,2011,38(1):28-30 【扩展库】
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关键词
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结肠癌
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肝转移
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模型
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裸鼠
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地址
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1.
南通大学第二附属医院普外科, 江苏, 南通, 226001
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南通大学病理教研室
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上海瑞金医院普外科
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江苏省南通大学附属医院普外科微创组
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语种
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中文 |
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文献类型
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研究性论文 |
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ISSN
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1000-8578 |
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学科
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肿瘤学 |
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基金
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国家自然科学基金资助项目
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文献收藏号
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CSCD:4121283
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参考文献 共
8
共1页
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1.
de Jong GM. Animal models for liver metastases of colorectal cancer: research review of preclinical studies in rodents.
J Surg Res,2009,154(1):167-176
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被引
3
次
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2.
邹忠东. 组织特异性CD/5-FC系统热化疗对裸鼠结肠癌肝转移的疗效.
中国肿瘤生物治疗杂志,2009,16(4):387-390
|
被引
1
次
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|
|
|
|
3.
Graf W. Induction and quantification of hepatic metastases from a human colonic cancer in the nude rat.
Eur J Surg Oncol,1992,18(6):608-614
|
被引
1
次
|
|
|
|
|
4.
Cai S R. A mouse model for developing treatment for secondary liver tumors.
Int J Oncol,2005,27(1):113-120
|
被引
6
次
|
|
|
|
|
5.
Nordlinger B. Experimental model of colon cancer: recurrences after surgery alone or associated with intraperitoneal5-fluorouracil chemotherapy.
Dis Colon Rectum,1991,34(8):658-663
|
被引
1
次
|
|
|
|
|
6.
许勤. 小鼠结肠腺癌肝转移模型的建立.
实用癌症杂志,2000,15(5):456-457
|
被引
14
次
|
|
|
|
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7.
Kozlowski JM. Metastatic behavior of human tumor cell lines grown in the nude mouse.
Cancer Res,1984,44(8):3522-3529
|
被引
18
次
|
|
|
|
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8.
陈陵际. 运用人癌裸小鼠移植瘤模型进行抗癌新药评价-- NCI和ECRTC临床前体内药效学研究方法和原则.
上海实验动物科学,2001,21(4):247-250
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被引
5
次
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