Rapid Determination of Ranitidine in Human Plasma by Liquid Chromatography-Tandem Mass Spectrometry and Its Application to a Clinical Pharmacokinetic Study
查看参考文献25篇
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文摘
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A rapid and sensitive assay based on high performance liquid chromatography-tandem mass spectrometry (LC-MS/MS)was developed for the determination of ranitidine(RAN)in human plasma with codeine as internal standard(IS).After protein precipitation with acetonitrile,the analyte and IS were separated on a Zorbax SB-Aq C18 column(150 mm×4.6 mm i.d.,5μm)eluted with a mobile phase consisting of methanol/acetonitrile/10 mmol/L ammonium acetate containing 1%formic acid(pH=2.4)(volume ratio 12.5:12.5:75)at a flow rate of 1.0 mL/min.Detection was performed by electrospray ionization in the positive ion mode followed by the multiple reaction monitoring(MRM)of the transitions of RAN at m/z 315.1→176.3 and of IS at m/z 300.1→165.1.The method was linear over a concentration range of 1―1000 ng/mL(r=0.9991)with a lower limit of quantitation(LLOQ)of 1 ng/mL and a limit of detection(LOD)of 0.3 ng/mL.Accuracy as relative error was from-0.01%to-1.7%and intra-day and inter-day precisions as relative standard deviation were≤8.9%and≤5.5%,respectively.The method was successfully applied to a pharmacokinetic study of ranitidine,getting a single oral dose(160 mg)to healthy volunteers |
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来源
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Chemical Research in Chinese Universities
,2010,26(6):910-914 【核心库】
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关键词
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Ranitidine
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Pharmacokinetics
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Human plasma
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Protein precipitation
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LC-MS/MS
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地址
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1.
Research Center for Drug Metabolism,College of Life Science,Jilin University, Changchun, 130021
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School of Pharmacy,University of Otago, New Zealand, Dunedin
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Second Clinical Hospital Affiliated to the Chinese Medical University, Shenyang, 110004
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语种
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英文 |
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文献类型
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研究性论文 |
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ISSN
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1005-9040 |
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学科
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化学 |
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基金
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国家863计划
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国家自然科学基金
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文献收藏号
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CSCD:4093582
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参考文献 共
25
共2页
|
|
1.
Grant SM.
Drugs,1989,37:801
|
被引
2
次
|
|
|
|
|
2.
Higuchi K.
Int.J.Clin.Pharmacol.Res,2005,25:187
|
被引
2
次
|
|
|
|
|
3.
Vakil N.
Aliment.Pharmacol.Ther,2006,23:649
|
被引
1
次
|
|
|
|
|
4.
Amendola R.
Acta Gastroenterol.Latinoam,2007,37:231
|
被引
1
次
|
|
|
|
|
5.
Mesquita MA.
World J.Gastroenterol,2005,11:3566
|
被引
1
次
|
|
|
|
|
6.
Gisbert JP.
Helicobacter,2005,10:157
|
被引
5
次
|
|
|
|
|
7.
Gschwend MH.
Arzneimittelforschun,2007,57:315
|
被引
1
次
|
|
|
|
|
8.
Zhou Q.
World J. Gastroenterol,2006,12:2742
|
被引
1
次
|
|
|
|
|
9.
Chung WG.
Jpn.J. Pharmacol,2000,84:213
|
被引
3
次
|
|
|
|
|
10.
Wong CF.
J.Chromatogr.B,1998,718:205
|
被引
1
次
|
|
|
|
|
11.
Kokoletsi MX.
J.Pharm.Biomed.Anal,2005,38:763
|
被引
3
次
|
|
|
|
|
12.
Ashiru DAI.
J.Chromatogr.B,2007,860:235
|
被引
1
次
|
|
|
|
|
13.
Li W.
J.Pharm.Biomed.Anal,2006,41:594
|
被引
2
次
|
|
|
|
|
14.
Perez CF.
J.Chromatogr.B,2003,795:141
|
被引
2
次
|
|
|
|
|
15.
do Nascimento TG.
J.Pharm. Biomed.Anal,2005,37:777
|
被引
2
次
|
|
|
|
|
16.
Khedr A.
J.Chromatogr.B,2008,862:175
|
被引
2
次
|
|
|
|
|
17.
Vinas P.
J. Chromatogr.B,1997,693:443
|
被引
1
次
|
|
|
|
|
18.
Kataoka H.
J.Chromatogr.B,1999,731:353
|
被引
6
次
|
|
|
|
|
19.
Zhang Y.
Clin.Chim.Acta,2008,398:105
|
被引
2
次
|
|
|
|
|
20.
Sun XH.
J.Chromatogr.B,2009,877:3953
|
被引
1
次
|
|
|
|
|
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