Promoter Hypermethylation of KiSS-1 Gene in Gastric Cancer
查看参考文献28篇
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文摘
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Objective:To investigate the association between KiSS-1 methylation and clinicopathological characteristics of gastric cancer and evaluate the role of peritoneal lavage fluid in detecting peritoneal metastases. Methods:The methylation status of KiSS-1 gene in 40 gastric cancer specimens,the corresponding adjacent normal mucosa,lymph nodes and peritoneal lavage fluid was investigated by methylation-spcific polymerase chain reaction(MS-PCR). Results:Aberrant methylation of KiSS-1 gene was detected in 55%(22/40)of the adjacent normal mucosa,82.5%(33/40)of gastric cancer specimens,80.95%(17/21)of the lymph nodes,and 42.5%(17/40) of peritoneal lavage fluid.Methylation in gastric carcinoma and lymphonode was more frequent than in non-neoplastic gastiric mucosa.Presence of KiSS-1 methylation in peritoneal lavage fluid was significantly correlated with tumor invasion(P=0.043).The accuracy of KiSS-1 methylation in peritoneal lavage fluid for diagnosing peritoneal metastasis was 70%,with a sensitivity of 77.8%and a specificity of 67.7%. Conclusion:Aberrant methylation of KiSS-1 gene is a common event in the occurrence and progression of gastric carcinoma,which may provide useful information for the early diagnosis of peritoneal metastases and a new therapy for gastric cancer |
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来源
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Chinese Journal of Cancer Research
,2010,22(4):280-284 【核心库】
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关键词
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Gastric cancer
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Methylation
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KiSS-1
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Peritoneal lavage fluid
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地址
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Department of Surgical Oncology,the First Affiliated Hospital,China Medical University, Shenyang, 110001
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语种
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英文 |
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文献类型
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研究性论文 |
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ISSN
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1000-9604 |
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学科
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肿瘤学 |
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基金
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国家自然科学基金
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Science Foundation for High Education Program of Liaoning Province
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文献收藏号
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CSCD:4075598
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